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Unravelling Plasma Extracellular Vesicle Diversity With Optimised Spectral Flow Cytometry [PDF]

Journal of Extracellular Biology
Extracellular vesicles (EVs) are crucial for intercellular communication and are found in various biological fluids. The identification and immunophenotyping of such small particles continue to pose significant challenges.
Daniela Boselli, Francesca Clemente, Simona Di Terlizzi, Christina Pagiatakis, Laura Papa, Genny Del Zotto, Chiara Villa, Giuseppe Alvise Ramirez, Norma Maugeri, Angelo A. Manfredi, Achille Anselmo   +10 more
doaj   +2 more sources

A compendium of single extracellular vesicle flow cytometry

Journal of Extracellular Vesicles, 2023
Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells, which are larger than EVs.
J. Welsh, G. Arkesteijn, Michel Bremer, Michael Cimorelli, F. Dignat-George, B. Giebel, A. Görgens, A. Hendrix, Martine Kuiper, R. Lacroix, J. Lannigan, T. V. van Leeuwen, Estefanía Lozano-Andrés, Shoaib Rao, S. Robert, L. de Rond, V. Tang, T. Tertel, Xiaomei Yan, M. Wauben, J. Nolan, Jennifer C. Jones, R. Nieuwland, E. van der Pol   +23 more
semanticscholar   +1 more source

Flow Cytometry: The Next Revolution

Cells, 2023
Unmasking the subtleties of the immune system requires both a comprehensive knowledge base and the ability to interrogate that system with intimate sensitivity.
J. Paul Robinson, R. Ostafe, S. Iyengar, Bartek Rajwa, Rainer Fischer   +4 more
semanticscholar   +1 more source

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

European Journal of Immunology, 2021
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell ...
A. Cossarizza, Hyun-Dong Chang, A. Radbruch, S. Abrignani, Richard K Addo, M. Akdiş, Immanuel Andrä, F. Andreata, F. Annunziato, E. Arranz, P. Bacher, S. Bari, V. Barnaba, Joana Barros-Martins, Dirk Baumjohann, C. Beccaria, D. Bernardo, Dominic A. Boardman, J. Borger, C. Böttcher, L. Brockmann, M. Burns, D. Busch, G. Cameron, Ilenia Cammarata, Antonino Cassotta, Yinshui Chang, F. Chirdo, Eleni Christakou, L. Čičin-Šain, Laura Cook, A. Corbett, Rebecca Cornelis, L. Cosmi, M. Davey, S. De Biasi, G. De Simone, Genny Del Zotto, Michael Delacher, F. Di Rosa, J. D. Santo, A. Diefenbach, Jun Dong, T. Dörner, R. Dress, C. Dutertre, S. Eckle, P. Eede, Maximilien Evrard, C. Falk, M. Feuerer, S. Fillatreau, A. Fiz-López, M. Follo, G. Foulds, J. Fröbel, N. Gagliani, G. Galletti, A. Gangaev, N. Garbi, J. A. Garrote, J. Geginat, N. Gherardin, L. Gibellini, F. Ginhoux, D. Godfrey, P. Gruarin, C. Haftmann, L. Hansmann, Christopher M. Harpur, A. Hayday, G. Heine, Daniela C Hernández, M. Herrmann, O. Hoelsken, Qing Huang, S. Huber, Johanna E. Huber, J. Huehn, M. Hundemer, W. Hwang, M. Iannacone, S. Ivison, H. Jäck, P. Jani, Baerbel Keller, N. Kessler, S. Ketelaars, Laura Knop, J. Knopf, H. Koay, K. Kobow, K. Kriegsmann, H. Kristyanto, A. Krueger, J. Kuehne, H. Kunze-Schumacher, P. Kvistborg, Immanuel Kwok, Daniela Latorre, D. Lenz, M. Levings, A. Lino, F. Liotta, H. Long, E. Lugli, K. N. MacDonald, L. Maggi, M. Maini, F. Mair, C. Manta, R. Manz, M. Mashreghi, A. Mazzoni, J. McCluskey, H. Mei, F. Melchers, S. Melzer, D. Mielenz, L. Monin, L. Moretta, G. Multhoff, L. Munoz, M. Muñoz-Ruiz, Franziska Muscate, Ambra Natalini, K. Neumann, L. Ng, Antonia Niedobitek, Jana Niemz, L. N. Almeida, Samuele Notarbartolo, L. Ostendorf, L. Pallett, Amit A. Patel, Gulce I Percin, G. Peruzzi, M. Pinti, A. Pockley, Katharina Pracht, I. Prinz, I. Pujol-Autonell, N. Pulvirenti, L. Quatrini, Kylie M. Quinn, H. Radbruch, H. Rhys, M. B. Rodrigo, C. Romagnani, C. Saggau, S. Sakaguchi, F. Sallusto, Lieke Sanderink, I. Sandrock, C. Schauer, A. Scheffold, H. Scherer, M. Schiemann, F. Schildberg, Kilian Schober, J. Schoen, W. Schuh, T. Schüler, A. Schulz, Sebastian R. Schulz, Julia Schulze, S. Simonetti, Jeeshan Singh, K. Sitnik, Regina Stark, Sarah C. Starossom, Christina Stehle, F. Szelinski, Leonard Tan, A. Tárnok, J. Tornack, T. Tree, Jasper J. P. van Beek, W. van de Veen, K. V. van Gisbergen, C. Vasco, Nikita Verheyden, Anouk von Borstel, Kirsten A. Ward-Hartstonge, K. Warnatz, Claudia Waskow, A. Wiedemann, A. Wilharm, J. Wing, O. Wirz, Jens Wittner, Jennie H. M. Yang, Juhao Yang   +192 more
semanticscholar   +1 more source

The in vitro micronucleus assay using imaging flow cytometry and deep learning

npj Systems Biology and Applications, 2021
The in vitro micronucleus (MN) assay is a well-established assay for quantification of DNA damage, and is required by regulatory bodies worldwide to screen chemicals for genetic toxicity.
Matthew A. Rodrigues, Christine E. Probst, Artiom Zayats, Bryan Davidson, Michael Riedel, Yang Li, Vidya Venkatachalam   +6 more
doaj   +1 more source

Flow cytometry: past and future.

BioTechniques, 2022
Flow cytometry is a single-cell technology that measures scatter and fluorescence to establish a set of unique cellular properties. Flow cytometry is used in many areas of science, in particular biotechnology and medicine, but also in industrial ...
J. Paul Robinson
semanticscholar   +1 more source

Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification

Communications Biology, 2021
Combining multi-angle (MA) light scatter detection with time-resolved pulse shape (PS) analysis for flow cytometry (FC), Kage et al. report MAPS-FC method for cell cycle analysis.
Daniel Kage, Kerstin Heinrich, Konrad v. Volkmann, Jenny Kirsch, Kristen Feher, Claudia Giesecke-Thiel, Toralf Kaiser   +6 more
doaj   +1 more source

Full spectrum flow cytometry and mass cytometry: A 32‐marker panel comparison

Cytometry Part A, 2022
High‐dimensional single‐cell data has become an important tool in unraveling the complexity of the immune system and its involvement in homeostasis and a large array of pathologies.
M. Jaimes, Michael D. Leipold, Geoffrey Kraker, E. Amir, H. Maecker, J. Lannigan   +5 more
semanticscholar   +1 more source

Current trends in flow cytometry automated data analysis software

Cytometry Part A, 2021
Automated flow cytometry (FC) data analysis tools for cell population identification and characterization are increasingly being used in academic, biotechnology, pharmaceutical, and clinical laboratories. The development of these computational methods is
Melissa Cheung, Jonathan J. Campbell, L. Whitby, R. Thomas, J. Braybrook, J. Petzing   +5 more
semanticscholar   +1 more source

MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments

Journal of Extracellular Vesicles, 2020
Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application to the analysis of EVs and other submicron particles has presented ...
J. Welsh, E. van der Pol, G. Arkesteijn, Michel Bremer, A. Brisson, F. Coumans, F. Dignat-George, E. Duggan, I. Ghiran, B. Giebel, A. Görgens, A. Hendrix, R. Lacroix, J. Lannigan, S. Libregts, Estefanía Lozano-Andrés, A. Morales-Kastresana, S. Robert, L. de Rond, T. Tertel, J. Tigges, O. De Wever, Xiaomei Yan, R. Nieuwland, M. Wauben, J. Nolan, Jennifer C. Jones   +26 more
semanticscholar   +1 more source