Results 1 to 10 of about 173,196 (125)

The in vitro micronucleus assay using imaging flow cytometry and deep learning

npj Systems Biology and Applications, 2021
The in vitro micronucleus (MN) assay is a well-established assay for quantification of DNA damage, and is required by regulatory bodies worldwide to screen chemicals for genetic toxicity.
Matthew A. Rodrigues, Christine E. Probst, Artiom Zayats, Bryan Davidson, Michael Riedel, Yang Li, Vidya Venkatachalam   +6 more
doaj   +1 more source

Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification

Communications Biology, 2021
Combining multi-angle (MA) light scatter detection with time-resolved pulse shape (PS) analysis for flow cytometry (FC), Kage et al. report MAPS-FC method for cell cycle analysis.
Daniel Kage, Kerstin Heinrich, Konrad v. Volkmann, Jenny Kirsch, Kristen Feher, Claudia Giesecke-Thiel, Toralf Kaiser   +6 more
doaj   +1 more source

An Early Myelosuppression in the Acute Mouse Sepsis Is Partly Outcome-Dependent

Frontiers in Immunology, 2021
Adult hematopoietic stem and progenitor cells (HSPCs) respond to bacterial infections by expansion to myeloid cells. Sepsis impairs this process by suppressing differentiation of stem cells subsequently contributing to an ineffective immune response ...
Tomasz Skirecki, Susanne Drechsler, Aldona Jeznach, Grażyna Hoser, Mohammad Jafarmadar, Jerzy Kawiak, Marcin F. Osuchowski   +6 more
doaj   +1 more source

A UV‐C LED‐based unit for continuous decontamination of the sheath fluid in a flow‐cytometric cell sorter

Engineering in Life Sciences, 2022
Aseptic cell sorting is challenging, especially when a flow‐cytometric cell sorter is not operated in a sterile environment. The sheath fluid system of a cell sorter may be contaminated with germs such as bacteria, yeasts, viruses, or fungi.
Jenny Kirsch, Kerstin Heinrich, Daniel Kage, Johannes Glaab, Benjamin Grothe, Konrad v. Volkmann, Toralf Kaiser   +6 more
doaj   +1 more source

A gain and dynamic range independent index to quantify spillover spread to aid panel design in flow cytometry

Scientific Reports, 2021
In conventional flowcytometry one detector (primary) is dedicated for one fluorochrome. However, photons usually end up in other detectors too (fluorescence spillover).
Debajit Bhowmick, Frank van Diepen, Anita Pfauth, Renaud Tissier, Michelle L. Ratliff   +4 more
doaj   +1 more source

A biology-driven deep generative model for cell-type annotation in cytometry [PDF]

, 2022
Cytometry enables precise single-cell phenotyping within heterogeneous populations. These cell types are traditionally annotated via manual gating, but this method suffers from a lack of reproducibility and sensitivity to batch-effect. Also, the most recent cytometers - spectral flow or mass cytometers - create rich and high-dimensional data whose ...
arxiv   +1 more source

Detection of Central Nervous System Infiltration by Myeloid and Lymphoid Hematologic Neoplasms Using Flow Cytometry Analysis: Diagnostic Accuracy Study

Frontiers in Medicine, 2018
IntroductionInfiltration of the central nervous system (CNS) by hematologic or lymphoid malignant cells can cause extensive neurological damage, be progressive and fatal.
Laiz Cameirão Bento, Rodolfo Patussi Correia, Anderson Marega Alexandre, Sonia Tsukasa Nosawa, Eduardo de Carvalho Pedro, Andressa da Costa Vaz, Daniela Schimidell, Gustavo Bruniera Peres Fernandes, Gustavo Bruniera Peres Fernandes, Carlos Augusto Senne Duarte, Carlos Augusto Senne Duarte, Rodrigo de Souza Barroso, Nydia Strachman Bacal, Nydia Strachman Bacal   +13 more
doaj   +1 more source

The Expression of IL-17, in Chronic Spontaneous Urticaria Is Linked to Semaphorin5A

Biomolecules, 2021
Background: Patients with chronic spontaneous urticaria (CSU), an autoimmune disorder, show increased skin expression of IL-17A and can benefit from treatment with the anti-IL-17A biologic secukinumab.
Matanis Lobna, Eiza Nasren, Sabag Adi, Bejar Jacob, Gimenez-Arnau Ana Maria, Maurer Marcus, Vadasz Zahava   +6 more
doaj   +1 more source

Rapid evaluation of T cell clonality in the diagnostic work-up of mature T cell neoplasms: TRBC1-based flow cytometric assay experience

Translational Oncology, 2022
The identification of mature T cell neoplasms by flow cytometry is often challenging, due to overlapping features with reactive T cells and limitations of currently available T cell clonality assays. The description of an antibody specific for one of two
Manuela Capone, Benedetta Peruzzi, Boaz Palterer, Sara Bencini, Alessandro Sanna, Benedetta Puccini, Luca Nassi, Benedetta Salvadori, Marinella Statello, Alessia Carraresi, Stefania Stefanelli, Chiara Orazzini, Barbara Minuti, Roberto Caporale, Francesco Annunziato   +14 more
doaj  

flowEMMi: an automated model-based clustering tool for microbial cytometric data

BMC Bioinformatics, 2019
Background Flow cytometry (FCM) is a powerful single-cell based measurement method to ascertain multidimensional optical properties of millions of cells. FCM is widely used in medical diagnostics and health research.
Joachim Ludwig, Christian Höner zu Siederdissen, Zishu Liu, Peter F. Stadler, Susann Müller   +4 more
doaj   +1 more source