Results 1 to 10 of about 5,219,938 (357)

A compendium of single extracellular vesicle flow cytometry

Journal of Extracellular Vesicles, 2023
Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells, which are larger than EVs.
J. Welsh, G. Arkesteijn, Michel Bremer, Michael Cimorelli, F. Dignat-George, B. Giebel, A. Görgens, A. Hendrix, Martine Kuiper, R. Lacroix, J. Lannigan, T. V. van Leeuwen, E. Lozano-Andrés, Shoaib Rao, S. Robert, L. de Rond, V. Tang, T. Tertel, Xiaomei Yan, M. Wauben, J. Nolan, Jennifer C. Jones, R. Nieuwland, Edwin van der Pol   +23 more
semanticscholar   +1 more source

Flow Cytometry: The Next Revolution

Cells, 2023
Unmasking the subtleties of the immune system requires both a comprehensive knowledge base and the ability to interrogate that system with intimate sensitivity.
J. Paul Robinson, R. Ostafe, S. Iyengar, Bartek Rajwa, Rainer Fischer   +4 more
semanticscholar   +1 more source

The in vitro micronucleus assay using imaging flow cytometry and deep learning

npj Systems Biology and Applications, 2021
The in vitro micronucleus (MN) assay is a well-established assay for quantification of DNA damage, and is required by regulatory bodies worldwide to screen chemicals for genetic toxicity.
Matthew A. Rodrigues, Christine E. Probst, Artiom Zayats, Bryan Davidson, Michael Riedel, Yang Li, Vidya Venkatachalam   +6 more
doaj   +1 more source

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

European Journal of Immunology, 2021
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell ...
A. Cossarizza, Hyun-Dong Chang, A. Radbruch, S. Abrignani, Richard K Addo, M. Akdiş, Immanuel Andrä, F. Andreata, F. Annunziato, E. Arranz, P. Bacher, S. Bari, V. Barnaba, Joana Barros-Martins, Dirk Baumjohann, C. Beccaria, D. Bernardo, Dominic A. Boardman, J. Borger, C. Böttcher, L. Brockmann, M. Burns, D. Busch, G. Cameron, Ilenia Cammarata, Antonino Cassotta, Yinshui Chang, F. Chirdo, Eleni Christakou, L. Čičin-Šain, Laura Cook, A. Corbett, Rebecca Cornelis, L. Cosmi, M. Davey, S. De Biasi, G. De Simone, Genny Del Zotto, Michael Delacher, F. Di Rosa, J. D. Santo, A. Diefenbach, Jun Dong, T. Dörner, R. Dress, C. Dutertre, S. Eckle, P. Eede, Maximilien Evrard, C. Falk, M. Feuerer, S. Fillatreau, A. Fiz-López, M. Follo, G. Foulds, J. Fröbel, N. Gagliani, G. Galletti, A. Gangaev, N. Garbi, J. A. Garrote, J. Geginat, N. Gherardin, L. Gibellini, F. Ginhoux, D. Godfrey, P. Gruarin, C. Haftmann, L. Hansmann, Christopher M. Harpur, A. Hayday, G. Heine, Daniela C Hernández, M. Herrmann, O. Hoelsken, Qing Huang, S. Huber, Johanna E. Huber, J. Huehn, M. Hundemer, W. Hwang, M. Iannacone, S. Ivison, H. Jäck, P. Jani, Baerbel Keller, N. Kessler, S. Ketelaars, Laura Knop, J. Knopf, H. Koay, K. Kobow, K. Kriegsmann, H. Kristyanto, A. Krueger, J. Kuehne, H. Kunze-Schumacher, P. Kvistborg, Immanuel Kwok, Daniela Latorre, D. Lenz, M. Levings, A. Lino, F. Liotta, H. Long, E. Lugli, K. N. MacDonald, L. Maggi, M. Maini, F. Mair, C. Manta, R. Manz, M. Mashreghi, A. Mazzoni, J. McCluskey, H. Mei, F. Melchers, S. Melzer, D. Mielenz, L. Monin, L. Moretta, G. Multhoff, L. Munoz, M. Muñoz-Ruiz, Franziska Muscate, Ambra Natalini, K. Neumann, L. Ng, Antonia Niedobitek, Jana Niemz, L. N. Almeida, Samuele Notarbartolo, L. Ostendorf, L. Pallett, Amit A. Patel, Gulce I Percin, G. Peruzzi, M. Pinti, A. Pockley, Katharina Pracht, I. Prinz, I. Pujol-Autonell, N. Pulvirenti, L. Quatrini, Kylie M. Quinn, H. Radbruch, H. Rhys, M. B. Rodrigo, C. Romagnani, C. Saggau, S. Sakaguchi, F. Sallusto, Lieke Sanderink, I. Sandrock, C. Schauer, A. Scheffold, H. Scherer, M. Schiemann, F. Schildberg, Kilian Schober, J. Schoen, W. Schuh, T. Schüler, A. Schulz, Sebastian R. Schulz, Julia Schulze, S. Simonetti, Jeeshan Singh, K. Sitnik, Regina Stark, Sarah C. Starossom, Christina Stehle, F. Szelinski, Leonard Tan, A. Tárnok, J. Tornack, T. Tree, Jasper J. P. van Beek, W. van de Veen, K. V. van Gisbergen, C. Vasco, Nikita Verheyden, Anouk von Borstel, Kirsten A. Ward-Hartstonge, K. Warnatz, Claudia Waskow, A. Wiedemann, A. Wilharm, J. Wing, O. Wirz, Jens Wittner, Jennie H. M. Yang, Juhao Yang   +192 more
semanticscholar   +1 more source

Rapid evaluation of T cell clonality in the diagnostic work-up of mature T cell neoplasms: TRBC1-based flow cytometric assay experience

Translational Oncology, 2022
The identification of mature T cell neoplasms by flow cytometry is often challenging, due to overlapping features with reactive T cells and limitations of currently available T cell clonality assays. The description of an antibody specific for one of two
Manuela Capone, Benedetta Peruzzi, Boaz Palterer, Sara Bencini, Alessandro Sanna, Benedetta Puccini, Luca Nassi, Benedetta Salvadori, Marinella Statello, Alessia Carraresi, Stefania Stefanelli, Chiara Orazzini, Barbara Minuti, Roberto Caporale, Francesco Annunziato   +14 more
doaj   +1 more source

Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification

Communications Biology, 2021
Combining multi-angle (MA) light scatter detection with time-resolved pulse shape (PS) analysis for flow cytometry (FC), Kage et al. report MAPS-FC method for cell cycle analysis.
Daniel Kage, Kerstin Heinrich, Konrad v. Volkmann, Jenny Kirsch, Kristen Feher, Claudia Giesecke-Thiel, Toralf Kaiser   +6 more
doaj   +1 more source

Flow cytometry: past and future.

BioTechniques, 2022
Flow cytometry is a single-cell technology that measures scatter and fluorescence to establish a set of unique cellular properties. Flow cytometry is used in many areas of science, in particular biotechnology and medicine, but also in industrial ...
J. Paul Robinson
semanticscholar   +1 more source

Analysis of extracellular vesicle DNA at the single‐vesicle level by nano‐flow cytometry

Journal of Extracellular Vesicles, 2022
It has been demonstrated recently that extracellular vesicles (EVs) carry DNA; however, many fundamental features of DNA in EVs (EV‐DNA) remain elusive.
Haisheng Liu, Ye Tian, Chengfeng Xue, Qian Niu, Chen Chen, Xiaomei Yan   +5 more
semanticscholar   +1 more source

Current trends in flow cytometry automated data analysis software

Cytometry Part A, 2021
Automated flow cytometry (FC) data analysis tools for cell population identification and characterization are increasingly being used in academic, biotechnology, pharmaceutical, and clinical laboratories. The development of these computational methods is
Melissa Cheung, Jonathan J. Campbell, L. Whitby, R. Thomas, J. Braybrook, J. Petzing   +5 more
semanticscholar   +1 more source

A UV‐C LED‐based unit for continuous decontamination of the sheath fluid in a flow‐cytometric cell sorter

Engineering in Life Sciences, 2022
Aseptic cell sorting is challenging, especially when a flow‐cytometric cell sorter is not operated in a sterile environment. The sheath fluid system of a cell sorter may be contaminated with germs such as bacteria, yeasts, viruses, or fungi.
Jenny Kirsch, Kerstin Heinrich, Daniel Kage, Johannes Glaab, Benjamin Grothe, Konrad v. Volkmann, Toralf Kaiser   +6 more
doaj   +1 more source