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Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) [PDF]
European Journal of Immunology, 2021 The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell ...
A. Cossarizza +192 more
semanticscholar +3 more sources
Flow Cytometry: An Overview [PDF]
Current Protocols in Immunology, 2018 AbstractFlow cytometry is a technology that provides rapid multi‐parametric analysis of single cells in solution. Flow cytometers utilize lasers as light sources to produce both scattered and fluorescent light signals that are read by detectors such as photodiodes or photomultiplier tubes.
K. McKinnon
openaire +4 more sources
A compendium of single extracellular vesicle flow cytometry
Journal of Extracellular Vesicles, 2023 Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells, which are larger than EVs.
J. Welsh +23 more
semanticscholar +1 more source
Flow Cytometry: The Next Revolution
Cells, 2023 Unmasking the subtleties of the immune system requires both a comprehensive knowledge base and the ability to interrogate that system with intimate sensitivity.
J. Paul Robinson +4 more
semanticscholar +1 more source
The in vitro micronucleus assay using imaging flow cytometry and deep learning
npj Systems Biology and Applications, 2021 The in vitro micronucleus (MN) assay is a well-established assay for quantification of DNA damage, and is required by regulatory bodies worldwide to screen chemicals for genetic toxicity.
Matthew A. Rodrigues +6 more
doaj +1 more source
Flow cytometry: past and future.
BioTechniques, 2022 Flow cytometry is a single-cell technology that measures scatter and fluorescence to establish a set of unique cellular properties. Flow cytometry is used in many areas of science, in particular biotechnology and medicine, but also in industrial ...
J. Paul Robinson
semanticscholar +1 more source
Communications Biology, 2021 Combining multi-angle (MA) light scatter detection with time-resolved pulse shape (PS) analysis for flow cytometry (FC), Kage et al. report MAPS-FC method for cell cycle analysis.
Daniel Kage +6 more
doaj +1 more source
Current trends in flow cytometry automated data analysis software
Cytometry Part A, 2021 Automated flow cytometry (FC) data analysis tools for cell population identification and characterization are increasingly being used in academic, biotechnology, pharmaceutical, and clinical laboratories. The development of these computational methods is
Melissa Cheung +5 more
semanticscholar +1 more source
Repression of the stress granule protein G3BP2 inhibits immune checkpoint molecule PD‐L1
Molecular Oncology, EarlyView., 2022 Mounting evidence suggests that cancer stemness and immunosuppression are related, but the underlying mechanisms are not clear. We previously reported that the stress granule‐associated protein G3BP2 is involved in regulating tumor‐initiating (stem) cells.
Yanhong Zhang +3 more
wiley +1 more source
An Early Myelosuppression in the Acute Mouse Sepsis Is Partly Outcome-Dependent
Frontiers in Immunology, 2021 Adult hematopoietic stem and progenitor cells (HSPCs) respond to bacterial infections by expansion to myeloid cells. Sepsis impairs this process by suppressing differentiation of stem cells subsequently contributing to an ineffective immune response ...
Tomasz Skirecki +6 more
doaj +1 more source