ROR1 is a receptor tyrosine kinase family member studied for its roles in development and cancer. Here we describe a protocol for analysis of ROR1 surface expression in acute lymphoblastic leukemia immortalized cell lines by flow cytometry.
Jeffrey W. Tyner, Vincent T Bicocca
openaire +4 more sources
IntroductionInfiltration of the central nervous system (CNS) by hematologic or lymphoid malignant cells can cause extensive neurological damage, be progressive and fatal.
Laiz Cameirão Bento+13 more
doaj +1 more source
Signal processing in slit-scan flow cytometry of cell conjugates [PDF]
The design and implementation of a real-time signal processing system for slit-scan flow cytometry is described. The system is used to measure the separate scatter and fluorescence peak heights of 2 adherent cells.
Bakker Schut+9 more
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The ciliary GTPase Arl13b regulates cell migration and cell cycle progression [PDF]
Acknowledgments We acknowledge Prof. Tamara Caspary from Emory University for kindly providing the cell lines, Linda Duncan from the University of Aberdeen Ian Fraser Cytometry Center for help with flow cytometry.
Clancy, Hannah+6 more
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The Expression of IL-17, in Chronic Spontaneous Urticaria Is Linked to Semaphorin5A
Background: Patients with chronic spontaneous urticaria (CSU), an autoimmune disorder, show increased skin expression of IL-17A and can benefit from treatment with the anti-IL-17A biologic secukinumab.
Matanis Lobna+6 more
doaj +1 more source
Procoagulant and platelet-derived microvesicle absolute counts determined by flow cytometry correlates with a measurement of their functional capacity [PDF]
Background: Flow cytometry is the most commonly used technology to measure microvesicles (MVs). Despite reported limitations of this technique, MV levels obtained using conventional flow cytometry have yielded many clinically relevant findings, such as ...
Berne Ferry+3 more
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Approaches to overcome flow cytometry limitations in the analysis of cells from veterinary relevant species [PDF]
BACKGROUND: Flow cytometry is a powerful tool for the multiparameter analysis of leukocyte subsets on the single cell level. Recent advances have greatly increased the number of fluorochrome-labeled antibodies in flow cytometry.
Debes, Gudrun F.+2 more
core +1 more source
Quantification of gliadin levels to the picogram level by flow cytometry [PDF]
Celiac disease is a widely prevalent enteropathy caused by intolerance to gliadin, one of the gluten proteins. We developed two methods for the analysis of gliadin levels. Both methods use flow cytometry and rat antibodies against a 16-residue peptide of
CAPPARELLI R+3 more
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A computational pipeline for the diagnosis of CVID patients [PDF]
Common variable immunodeficiency (CVID) is one of the most frequently diagnosed primary antibody deficiencies (PADs), a group of disorders characterized by a decrease in one or more immunoglobulin (sub) classes and/or impaired antibody responses caused ...
Bogaert, Delfien+6 more
core +2 more sources
Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and exosomes [PDF]
Introduction: In recent years, there has been an exponential increase in the number of studies aiming to understand the biology of exosomes, as well as other extracellular vesicles.
Berent-Maoz B+28 more
core +2 more sources