Results 21 to 30 of about 5,462,613 (380)

Virtual-freezing fluorescence imaging flow cytometry

open access: yesNature Communications, 2020
By virtue of the combined merits of flow cytometry and fluorescence microscopy, imaging flow cytometry (IFC) has become an established tool for cell analysis in diverse biomedical fields such as cancer biology, microbiology, immunology, hematology, and ...
H. Mikami   +21 more
semanticscholar   +1 more source

Signal processing in slit-scan flow cytometry of cell conjugates [PDF]

open access: yes, 1993
The design and implementation of a real-time signal processing system for slit-scan flow cytometry is described. The system is used to measure the separate scatter and fluorescence peak heights of 2 adherent cells.
Bakker Schut   +9 more
core   +2 more sources

Phenotyping renal leukocyte subsets by four-color flow cytometry: Characterization of chemokine receptor expression [PDF]

open access: yes, 2003
To investigate mechanisms of cell-mediated injury in renal inflammatory disease it is critical to determine the surface phenotype of infiltrating renal leukocyte subsets.
Anders, Hans-Joachim   +5 more
core   +1 more source

The Expression of IL-17, in Chronic Spontaneous Urticaria Is Linked to Semaphorin5A

open access: yesBiomolecules, 2021
Background: Patients with chronic spontaneous urticaria (CSU), an autoimmune disorder, show increased skin expression of IL-17A and can benefit from treatment with the anti-IL-17A biologic secukinumab.
Matanis Lobna   +6 more
doaj   +1 more source

Panel Design and Optimization for High‐Dimensional Immunophenotyping Assays Using Spectral Flow Cytometry

open access: yesCurrent Protocols in Cytometry, 2020
Technological advances in fluorescence flow cytometry and an ever‐expanding understanding of the complexity of the immune system have led to the development of large (20+ parameters) flow cytometry panels.
L. Ferrer-Font   +5 more
semanticscholar   +1 more source

Nanosecond electric pulses penetrate the nucleus and enhance speckle formation [PDF]

open access: yes, 2007
Nanosecond electric pulses generate nanopores in the interior membranes of cells and modulate cellular functions. Here, we used confocal microscopy and flow cytometry to observe Smith antigen antibody (Y12) binding to nuclear speckles, known as small ...
Beebe, S J   +9 more
core   +1 more source

Comparison of methods for in-house screening of HLA*B57:01 to prevent abacavir hypersensitivity in HIV-1 care [PDF]

open access: yes, 2015
Abacavir is a nucleoside reverse transcriptase inhibitor used as part of combination antiretroviral therapy in HIV-1-infected patients. Because this drug can cause a hypersensitivity reaction that is correlated with the presence of the HLA-B*57:01 ...
Bonczkowski, Pawel   +18 more
core   +4 more sources

Evaluation of Exosome Proteins by on‐Bead Flow Cytometry

open access: yesCytometry Part A, 2020
Exosomes, recently re‐named “small extracellular vesicles” or “sEV,” are emerging as an intercellular communication system. Quantification of the molecular cargo exosomes carry by on‐bead flow cytometry is needed for defining their role in information ...
M. Theodoraki   +4 more
semanticscholar   +1 more source

Monitoring CAR T‐cells using flow cytometry

open access: yesCytometry. Part B, Clinical cytometry, 2020
Chimeric antigen receptor (CAR) T‐cell therapy is considered as a major scientific breakthrough in cancer immunotherapy. The success of adoptive CAR T‐cell therapy for cancer has inspired researchers to expand indications into the area of solid tumors ...
J. Demaret   +7 more
semanticscholar   +1 more source

Approaches to overcome flow cytometry limitations in the analysis of cells from veterinary relevant species [PDF]

open access: yes, 2020
BACKGROUND: Flow cytometry is a powerful tool for the multiparameter analysis of leukocyte subsets on the single cell level. Recent advances have greatly increased the number of fluorochrome-labeled antibodies in flow cytometry.
Debes, Gudrun F.   +2 more
core   +1 more source

Home - About - Disclaimer - Privacy