AutoSpill is a principled framework that simplifies the analysis of multichromatic flow cytometry data. [PDF]
, 2021 Compensating in flow cytometry is an unavoidable challenge in the data analysis of fluorescence-based flow cytometry. Even the advent of spectral cytometry cannot circumvent the spillover problem, with spectral unmixing an intrinsic part of such systems.
Botzki, Alexander +11 more
core +1 more source
Translational Oncology, 2022 The identification of mature T cell neoplasms by flow cytometry is often challenging, due to overlapping features with reactive T cells and limitations of currently available T cell clonality assays. The description of an antibody specific for one of two
Manuela Capone +14 more
doaj
Procoagulant and platelet-derived microvesicle absolute counts determined by flow cytometry correlates with a measurement of their functional capacity [PDF]
, 2014 Background: Flow cytometry is the most commonly used technology to measure microvesicles (MVs). Despite reported limitations of this technique, MV levels obtained using conventional flow cytometry have yielded many clinically relevant findings, such as ...
Berne Ferry +3 more
core +2 more sources
The ciliary GTPase Arl13b regulates cell migration and cell cycle progression [PDF]
, 2016 Acknowledgments We acknowledge Prof. Tamara Caspary from Emory University for kindly providing the cell lines, Linda Duncan from the University of Aberdeen Ian Fraser Cytometry Center for help with flow cytometry.
Clancy, Hannah +6 more
core +2 more sources
flowEMMi: an automated model-based clustering tool for microbial cytometric data
BMC Bioinformatics, 2019 Background Flow cytometry (FCM) is a powerful single-cell based measurement method to ascertain multidimensional optical properties of millions of cells. FCM is widely used in medical diagnostics and health research.
Joachim Ludwig +4 more
doaj +1 more source
Multispectral imaging flow cytometry reveals distinct frequencies of γ-H2AX foci induction in DNA double strand break repair defective human cell lines [PDF]
, 2011 Copyright @ 2012 International Society for Advancement of Cytometry. The article can be accessed from the links below.This article has been made available through the Brunel Open Access Publishing Fund.The measurement of γ-H2AX foci induction in cells ...
Bottley, G +6 more
core +1 more source
PLoS Computational Biology, 2013 Flow cytometry bioinformatics is the application of bioinformatics to flow cytometry data, which involves storing, retrieving, organizing, and analyzing flow cytometry data using extensive computational resources and tools. Flow cytometry bioinformatics requires extensive use of and contributes to the development of techniques from computational ...
Kieran O'Neill +3 more
openaire +5 more sources
Activation of human NK cells by Plasmodium-infected red blood cells.
, 2012 This chapter describes a protocol to assess activation of human NK cells following in vitro stimulation with malaria-infected red blood cells. Activation is assessed by flow cytometry, staining for cell surface expression of CD69 and accumulation of ...
Horowitz, Amir, Riley, Eleanor M
core +1 more source
Quantification of gliadin levels to the picogram level by flow cytometry [PDF]
, 2005 Celiac disease is a widely prevalent enteropathy caused by intolerance to gliadin, one of the gluten proteins. We developed two methods for the analysis of gliadin levels. Both methods use flow cytometry and rat antibodies against a 16-residue peptide of
CAPPARELLI R +3 more
core +1 more source
Human Neutrophil Antigen Genotype and Allele Frequencies in Iranian Blood Donors
Journal of Immunology Research, 2022 Objective. Human neutrophil antigens (HNAs) can be targeted by HNA-allo antibodies and cause a variety of clinical conditions such as transfusion-related acute lung injury (TRALI) and neonatal alloimmune neutropenia (NAIN).
Behnaz Esmaeili +5 more
doaj +1 more source