Results 31 to 40 of about 5,219,938 (357)

Pathogenic Germline PALB2 and RAD50 Variants in Patients With Relapsed Ewing Sarcoma

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Approximately 10% of patients with Ewing sarcoma (EwS) have pathogenic germline variants. Here, we report two cases: first, a novel germline pathogenic variant in partner and localizer of BRCA2 (PALB2) in a patient with a late EwS relapse. Its impact on homologous recombination is demonstrated, and breast cancer risk is discussed.
Molly Mack, A. Carolina Tufino, Mohd Azrin Jamalruddin, Sreya Dey, Yinghong Pan, Elina Mukherjee, Jessica D. Daley, Julia Meade, Mahmoud Aarabi, Lisa Maurer, James Cooper, Kara A. Bernstein, Kelly M. Bailey   +12 more
wiley   +1 more source

Cell sorting based on pulse shapes from angle resolved detection of scattered light

Communications Biology
Flow cytometry is a key technology for the analysis and sorting of cells or particles at high throughput. Conventional and current flow cytometry is primarily based on fluorescent stains to detect the cells of interest.
Daniel Kage, Andrej Eirich, Kerstin Heinrich, Jenny Kirsch, Jan Popien, Alexander Wolf, Konrad v. Volkmann, Hyun-Dong Chang, Toralf Kaiser   +8 more
doaj   +1 more source

Subcutaneous immunotherapy induces alterations in monocytes and dendritic cells homeostasis in allergic rhinitis patients

Allergy, Asthma & Clinical Immunology, 2018
Background Specific subcutaneous immunotherapy (SCIT) can achieve long-term remission in patients with allergic rhinitis (AR) through complex and still unknown mechanisms.
Letícia Sousa, Carmen Martín-Sierra, Celso Pereira, Graça Loureiro, Beatriz Tavares, Susana Pedreiro, António Martinho, Artur Paiva   +7 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

A quick, reliable, and automated method for fat cell sizing

Journal of Lipid Research, 2003
Mean diameters of fat cells from abdominal tissues from 31 volunteers were determined by three methods based on fat cell isolation after collagenase digestion and methylene blue staining.
Yourka D. Tchoukalova, Deborah A. Harteneck, R.A. Karwoski, J. Tarara, Michael D. Jensen   +4 more
doaj   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

HPLC and flow cytometry combined approach for HbF analysis in fetomaternal haemorrhage evaluation

Practical Laboratory Medicine
Introduction: Recently, a flow cytometric (FC) based test has been developed for detection of circulating fetal cells to replace the less accurate and reproducible Kleihauer-Betke test.FC test is easier to perform, it can distinguish the origin of fetal ...
Benedetta Peruzzi, Serena Guerrieri, Tiziana Biagioli, Luisa Lanzilao, Sara Pratesi, Sara Bencini, Marinella Statello, Alessia Carraresi, Stefania Stefanelli, Martina Tonelli, Marco Brogi, Manuela Capone, Alessio Mazzoni, Anna Maria Grazia Gelli, Alessandra Fanelli, Roberto Caporale, Francesco Annunziato   +16 more
doaj   +1 more source

Human Neutrophil Antigen Genotype and Allele Frequencies in Iranian Blood Donors

Journal of Immunology Research, 2022
Objective. Human neutrophil antigens (HNAs) can be targeted by HNA-allo antibodies and cause a variety of clinical conditions such as transfusion-related acute lung injury (TRALI) and neonatal alloimmune neutropenia (NAIN).
Behnaz Esmaeili, Behnaz Bayat, Atefe Alirezaee, Mona Delkhah, Mohammad Reza Mehdizadeh, Zahra Pourpak   +5 more
doaj   +1 more source

Estimation of Microbial Viability Using Flow Cytometry

Current Protocols in Cytometry, 2020
For microorganisms in particular, viability is a term that is difficult to define and a state consequently difficult to measure. The traditional (and gold standard) usage equates viability and culturability (i.e., the ability to multiply) but the process
Hazel Davey, Stéphane Guyot
semanticscholar   +1 more source

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source