Results 51 to 60 of about 3,604,557 (398)

Protonophore activity of short‐chain fatty acids induces their intracellular accumulation and acidification

open access: yesFEBS Letters, EarlyView.
The protonated form of butyrate, as well as other short‐chain fatty acids (SCFAs), is membrane permeable. In acidic extracellular environments, this can lead to intracellular accumulation of SCFAs and cytosolic acidification. This phenomenon will be particularly relevant in acidic environments such as the large intestine or tumor microenvironments ...
Muwei Jiang   +2 more
wiley   +1 more source

A multidimensional phasor approach reveals LAURDAN photophysics in NIH-3T3 cell membranes

open access: yesScientific Reports, 2017
Mammalian cell membranes have different phospholipid composition and cholesterol content, displaying a profile of fluidity that depends on their intracellular location. Among the dyes used in membrane studies, LAURDAN has the advantage to be sensitive to
Leonel Malacrida   +2 more
doaj   +1 more source

Fluorescence Microscopy [PDF]

open access: yesCold Spring Harbor Protocols, 2014
Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters remain similar, microscope design varies with the aim of increasing image contrast and spatial resolution.
Sanderson, Michael J   +3 more
openaire   +4 more sources

Modifications in FLAP's second cytosolic loop influence 5‐LOX interaction, inhibitor binding, and leukotriene formation

open access: yesFEBS Letters, EarlyView.
The enzyme 5‐lipoxygenase (5‐LOX) catalyzes the first step in the biosynthesis of leukotrienes (LTs) involved in inflammatory pathophysiology. After cellular stimulation, 5‐LOX translocates to the nucleus, interacting with the 5‐LOX‐activating protein (FLAP) to form LTA4 from arachidonic acid (AA).
Erik Romp   +5 more
wiley   +1 more source

Surface plasmon resonance based colorimetric probe for vitamin B1 detection: Applications to bio-fluid analysis

open access: yesCase Studies in Chemical and Environmental Engineering, 2023
This study reports simple analytical approach for thiamine (Vitamin B1) detection based on induced aggregation and alternation in colorimetric properties of gold nanoparticles (AuNPs), which was synthesized through citrate reduction approach. Furthermore,
Uttam R. Kondekar   +8 more
doaj  

The thioredoxin‐like and one glutaredoxin domain are required to rescue the iron‐starvation phenotype of HeLa GLRX3 knock out cells

open access: yesFEBS Letters, EarlyView.
Glutaredoxin (Grx) 3 proteins contain a thioredoxin domain and one to three class II Grx domains. These proteins play a crucial role in iron homeostasis in eukaryotic cells. In human Grx3, at least one of the two Grx domains, together with the thioredoxin domain, is essential for its function in iron metabolism.
Laura Magdalena Jordt   +4 more
wiley   +1 more source

Linear Combination Properties of the Phasor Space in Fluorescence Imaging

open access: yesSensors, 2022
The phasor approach to fluorescence lifetime imaging, and more recently hyperspectral fluorescence imaging, has increased the use of these techniques, and improved the ease and intuitiveness of the data analysis.
Belén Torrado   +2 more
doaj   +1 more source

Histologic determination of apocrine gland depth in patients with axillary osmidrosis [PDF]

open access: yesArchives of Aesthetic Plastic Surgery, 2020
Background Subdermal shaving is a surgical procedure for the treatment of axillary osmidrosis. In this procedure, a direct axillary incision is made, and the apocrine glands are removed.
Cheol-Heum Park   +4 more
doaj   +1 more source

An Intermediate State of the {gamma}-Aminobutyric Acid Transporter GAT1 Revealed by Simultaneous Voltage Clamp and Fluorescence [PDF]

open access: yes, 2000
The rat {gamma}-aminobutyric acid transporter GAT1 expressed in Xenopus oocytes was labeled at Cys74, and at one or more other sites, by tetramethylrhodamine-5-maleimide, without significantly altering GAT1 function.
Farley, Robert A.   +2 more
core   +1 more source

Quenching of fluorescence of aromatic molecules by graphene due to electron transfer

open access: yes, 2010
Investigations on the fluorescence quenching of graphene have been carried out with two organic donor molecules, pyrene butanaoic acid succinimidyl ester (PyBS, I) and oligo(p-phenylenevinylene) methyl ester (OPV-ester, II).
George, Subi J   +4 more
core   +1 more source

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