Results 1 to 10 of about 927,051 (305)
POT1 Stability and Binding Measured by Fluorescence Thermal Shift Assays [PDF]
PLOS ONE, 2021 AbstractThe protein POT1 (Protection of Telomeres 1) is an integral part of the shelterin complex that protects the ends of human chromosomes from degradation or end fusions. It is the only component of shelterin that binds single-stranded DNA. We describe here the application of two separate fluorescent thermal shift assays (FTSA) that provide ...
Lynn W. DeLeeuw +7 more
openaire +4 more sources
Insects, 2021 Olfaction is essential in some behaviors of honeybee, such as nursing, foraging, attracting a mate, social communication, and kin recognition. OBPs (odorant binding proteins) play a key role in the first step of olfactory perception.
Huiting Zhao +4 more
doaj +1 more source
Journal of Integrative Agriculture, 2022 Chemosensory proteins (CSPs) are important molecular components of the insect olfactory system, which are involved in capturing, binding, and transporting hydrophobic odour molecules across the sensillum in sensillar lymph in regulating insect behavior ...
Muhammad Irfan WARIS +5 more
doaj +1 more source
Novel Fluorescence Competition Assay for Retinoic Acid Binding Proteins [PDF]
ACS Medicinal Chemistry Letters, 2018 Vitamin A derived retinoid compounds have multiple, powerful roles in the cellular growth and development cycle and, as a result, have attracted significant attention from both academic and pharmaceutical research in developing and characterizing synthetic retinoid analogues.
Charles W. E. Tomlinson +4 more
openaire +4 more sources
Applying a high-throughput fluorescence polarization assay for the discovery of chemical probes blocking La:RNA interactions in vitro and in cells. [PDF]
PLoS ONE, 2017 The RNA-binding protein La is overexpressed in a number of tumor tissues and is thought to support tumorigenesis by binding to and facilitating the expression of mRNAs encoding tumor-promoting and anti-apoptotic factors.
Gunhild Sommer +5 more
doaj +1 more source
Characterization and Functional Analysis of OcomOBP7 in Ophraella communa Lesage
Insects, 2023 The olfactory system plays a key role in various insect behaviors, and odorant-binding proteins participate in the first step of the olfactory process. Ophraella communa Lesage is an oligophagous phytophagous insect that is a specific biological control ...
Yang Yue +6 more
doaj +1 more source
Frontiers in Molecular Biosciences, 2023 Dopamine receptors are G-protein-coupled receptors that are connected to severe neurological disorders. The development of new ligands targeting these receptors enables gaining a deeper insight into the receptor functioning, including binding mechanisms,
Maris-Johanna Tahk +10 more
doaj +1 more source
AFBI assay – Aptamer Fluorescence Binding and Internalization assay for cultured adherent cells [PDF]
Methods, 2016 The SELEX (Systematic Evolution of Ligands by Exponential Enrichment) process allows for the enrichment of DNA or RNA aptamers from a complex nucleic acid library that are specific for a target molecule. The SELEX process has been adapted from identifying aptamers in vitro using recombinant target protein to cell-based methodologies (Cell-SELEX), where
William H, Thiel, Paloma H, Giangrande
openaire +2 more sources
Development of a quantitative fluorescence-based ligand-binding assay [PDF]
Scientific Reports, 2016 AbstractA major goal of biology is to develop a quantitative ligand-binding assay that does not involve the use of radioactivity. Existing fluorescence-based assays have a serious drawback due to fluorescence quenching that accompanies the binding of fluorescently-labeled ligands to their receptors. This limitation of existing fluorescence-based assays
Breen, Conor +2 more
openaire +3 more sources
High-throughput fluorescence assay for membrane-protein interaction
Journal of Lipid Research, 2013 Membrane-protein interaction plays key roles in a wide variety of biological processes. Although various methods have been employed to measure membrane binding of soluble proteins, a robust high-throughput assay that is universally applicable to all ...
Hyunjin Kim +2 more
doaj +1 more source