Results 11 to 20 of about 927,051 (305)

Evaluation of Phage Display Discovered Peptides as Ligands for Prostate-Specific Membrane Antigen (PSMA) [PDF]

, 2013
The aim of this study was to identify potential ligands of PSMA suitable for further development as novel PSMA-targeted peptides using phage display technology. The human PSMA protein was immobilized as a target followed by incubation with a 15-mer phage
A Ghosh, AC Pawlyk, AM Quinn, CW Diehnelt, D Hessels, D Shen, DB Sodee, Duanwen Shen, E Basch, F Yi, Fei Xie, GA Lazar, H Liu, H Liu, H-S Lim, HD Agnew, IM Thompson, Irina U. Agoulnik, J Yu, JA Arter, JR Mesters, JR Newton, KA Noren, MI Davis, MI Milowsky, MJ Morris, MJ Morris, MJ Roobol, NH Bander, RK Nam, S Aggarwal, S Kim, S Minner, SE Lupold, SM Hillier, SR Banerjee, SR Banerjee, SR Banerjee, SR Banerjee, SS Chang, SZ Li, TJ Wigle, V Humblet, W. Barry Edwards, Y Chen, Y Chen, Y Chen, Z Xiao, ZY Zhu   +48 more
core   +14 more sources

Microspinning: Local Surface Mixing via Rotation of Magnetic Microparticles for Efficient Small-Volume Bioassays [PDF]

, 2020
The need for high-throughput screening has led to the miniaturization of the reaction volume of the chamber in bioassays. As the reactor gets smaller, surface tension dominates the gravitational or inertial force, and mixing efficiency decreases in small-
Cho, Seongkyu, Jeong, Hyun Yong, Jeong, Yunjin, Kang, Taehun, Kim, Jiyun, Kim, Min Chang, Kim, Su Deok, Koo, Jeong Woo, Kwon, Sunghoon, Lee, Amos Chungwon, Lee, Changhee, Lee, Daewon, Oh, Dong Yoon, Song, Seo Woo   +13 more
core   +1 more source

RyR1-targeted drug discovery pipeline integrating FRET-based high-throughput screening and human myofiber dynamic Ca2+ assays. [PDF]

, 2020
Elevated cytoplasmic [Ca2+] is characteristic in severe skeletal and cardiac myopathies, diabetes, and neurodegeneration, and partly results from increased Ca2+ leak from sarcoplasmic reticulum stores via dysregulated ryanodine receptor (RyR) channels ...
Bers, Donald M, Cornea, Razvan L, Janicek, Kevyn A, Launikonis, Bradley S, Rebbeck, Robyn T, Singh, Daniel P, Thomas, David D   +6 more
core   +2 more sources

Interactions of Cisplatin and Daunorubicin at the chromatin level [PDF]

, 2020
Unexpectedly, the widely used anticancer agents Cisplatin (Cis-Pt) and Daunorubicin (Dauno) exhibited cell type- and concentration-dependent synergy or antagonism in vitro.
Bacsó, Zsolt, Firouzi Niaki, Erfaneh, Imre, László, Nánási, Péter Jr., Szabó, Gábor, Tarapcsák, Szabolcs, Van Acker, Thibaut, Vanhaecke, Frank   +7 more
core   +2 more sources

Sensing ligand binding to a clinically relevant lectin by tryptophan fluorescence anisotropy [PDF]

, 2011
Increasing insights into the involvement of endogenous lectins in disease processes fuel the interest to develop potent inhibitors. As a consequence, robust assay procedures are required. Due to their activity as adhesion/growth-regulatory effectors this
André, Sabine, Büchner, Claudia, Doose, Sören, Gabius, Hans-Joachim, Göhler, Antonia, Kaltner, Herbert   +5 more
core   +1 more source

Identification and Evolution of a Natural Tetr Protein Based on Molecular Docking and Development of a Fluorescence Polari-Zation Assay for Multi-Detection of 10 Tetracyclines in Milk

Foods, 2022
In this study, the identity of our recently produced natural TetR protein was identified by using the LC-ESI-MS/MS technique, and its recognition mechanisms, including the binding pocket, contact amino acids, intermolecular forces, binding sites, binding
Wanqiu Xia, Jing Liu, Jianping Wang
doaj   +1 more source

Surfactant-Assisted Label-Free Fluorescent Aptamer Biosensors and Binding Assays

Biosensors, 2023
Using DNA staining dyes such as SYBR Green I (SGI) and thioflavin T (ThT) to perform label-free detection of aptamer binding has been performed for a long time for both binding assays and biosensor development. Since these dyes are cationic, they can also adsorb to the wall of reaction vessels leading to unstable signals and even false interpretations ...
Hanxiao Zhang, Albert Zehan Li, Juewen Liu   +2 more
openaire   +3 more sources

Proton Motive Force-Dependent Hoechst 33342 Transport by the ABC Transporter LmrA of Lactococcus lactis [PDF]

, 2005
The fluorescent compound Hoechst 33342 is a substrate for many multidrug resistance (MDR) transporters and is widely used to characterize their transport activity.
Abbreviations ABC, Chen M., Doerrler W. T., Ecker G. F., Gajic O., Haugland R. P., Higuchi R., Ketchum C. J., Kuipers O. P., Lalande M. E., Lanzetta P. A., Lewinson O., Linnett P. E., Lubelski J., Lubelski J., Margolles A., Mazurkiewicz P., Orelle C., Paternostre M. T., Poelarends G. J., Putman M., Sakamoto K., Sambrook J., Shapiro A. B., Shapiro A. B., Shapiro A. B., Shapiro A. B., Sharma S., Steinfels E., Urbatsch I. L., van Veen H. W., van Veen H. W., van Veen H. W., Venter H., Viitanen P., Yu J. L.   +35 more
core   +2 more sources

Assessing the Binding and Endocytosis Activity of Cellular Receptors Using GFP-Ligand Fusions

BioTechniques, 2000
We have developed a simple scheme for characterizing ligand-receptor binding and post-binding activity on living cells. Our approach makes use of green fluorescent protein (GFP) as an auto-fluorescent tag to label protein ligands. We have constructed GFP-
Lali K. Medina-Kauwe, Vivian Leung, Louis Wu, Larry Kedes   +3 more
doaj   +1 more source

Imaging beads-retained prey assay for rapid and quantitative protein-protein interaction. [PDF]

PLoS ONE, 2013
Conventional Western blot based pull-down methods involve lengthy and laborious work and the results are generally not quantitative. Here, we report the imaging beads-retained prey (IBRP) assay that is rapid and quantitative in studying protein-protein ...
Yan Zhou, Wanjin Hong, Lei Lu
doaj   +1 more source