Results 251 to 260 of about 927,051 (305)

Screening of FDA-Approved Small Molecules to Discover Inhibitors of the <i>Pseudomonas aeruginosa</i> Quorum-Sensing Enzyme, PqsE. [PDF]

Biochemistry
Jones HA, Baxter MJ, Zimmermann N, Li A, Perrault Uptmor KA, Taylor IR.   +5 more
europepmc   +1 more source

Mechanistic basis for relaxation of DNA supercoils by human topoisomerase IIIα-RMI1-RMI2. [PDF]

Proc Natl Acad Sci U S A
Spakman D, Biebricher AS, Bizard AH, Hickson ID, Peterman EJG, Wuite GJL, King GA.   +6 more
europepmc   +1 more source

mSumireF a Monomeric Violet Fluorescent Protein. [PDF]

ACS Bio Med Chem Au
Kress J, Kim S, Bryant C, Lopez-Lopez EC, Zha J, Tantama M.   +5 more
europepmc   +1 more source

Quantum Spin Detection in Microfiltration Immunoassays for Ultrasensitive and High-Throughput Diagnostics. [PDF]

Anal Chem
Le TN, Lam XM, Tang YX, Hui YY, Liu AJ, Chang HC.   +5 more
europepmc   +1 more source

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Sterol Binding Assay Using Surface Plasmon Fluorescence Spectroscopy

Analytical Chemistry, 2005
We describe the design of a novel in vitro assay to study the interaction of soluble proteins with small hydrophobic sterol ligands. The sterol molecules are incorporated in an artificial membrane system in order to mimic their arrangement found in a biomembrane.
Wiltschi, B., Schober, M., Kohlwein, S., Oesterhelt, D., Sinner, E.   +4 more
openaire   +3 more sources

A Fluorescence Polarization Based Src-SH2 Binding Assay

Analytical Biochemistry, 1997
The tyrosine kinase pp60c.src has been implicated as being a potential therapeutic target in several human diseases including cancer and osteoporosis. An important region within this kinase is the SH2 domain (Src homology 2) which binds to phosphorylated tyrosine residues contained within specific peptide sequences.
B A, Lynch, K A, Loiacono, C L, Tiong, S E, Adams, I A, MacNeil   +4 more
openaire   +2 more sources

A Fluorescence Anisotropy–Based Myt1 Kinase Binding Assay

ASSAY and Drug Development Technologies, 2014
Abstract The human Myt1 kinase is a regulator of Cdk1/CycB and, hence, important for the G2/M transition in the cell cycle. It may act as a target for drug development, but suitable assay systems for assessing potential inhibitors are lacking so far. Herein, we describe the rational development of a fluorescence anisotropy-based kinase binding assay. A
Alexander, Rohe, Claudia, Henze, Frank, Erdmann, Wolfgang, Sippl, Matthias, Schmidt   +4 more
openaire   +2 more sources

Phallotoxin and actin binding assay by fluorescence enhancement

Analytical Biochemistry, 1992
The fluorescence of five fluorophores conjugated to phallotoxins was found to be specifically enhanced upon binding to F-actin in a polymerizing buffer. Rhodamine phalloidin had the greatest fluorescence enhancement of ninefold. The fluorescence titration of rhodamine phalloidin by actin was shown to be consistent with stoichiometric binding.
Z J, Huang, R P, Haugland, W M, You, R P, Haugland   +3 more
openaire   +2 more sources