Results 261 to 270 of about 927,051 (305)

New fluorescent probes for ligand-binding assays of odorant-binding proteins

Biochemical and Biophysical Research Communications, 2014
Fluorescence-linked binding assays allow determination of dissociation constants at equilibrium and have recently become increasingly popular, thanks to their ease of operation. Currently used probes, such as 1-aminoanthracene and N-phenyl-1-naphthylamine, are excited and emit in the ultraviolet region, but alternative ligands operating in the visible ...
Rosa, Mastrogiacomo, Immacolata, Iovinella, Elio, Napolitano   +2 more
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A High-Throughput STAT Binding Assay Using Fluorescence Polarization

Analytical Biochemistry, 1997
STAT (signal transducers and activators of transcription) is a class of transcription factors that are activated upon cytokine or growth factor binding to cell surface receptors. Activated STAT proteins dimerize, translocate into the nucleus, and activate transcription, leading to various immune responses.
P, Wu, M, Brasseur, U, Schindler
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Fluorescent assay for riboflavin binding to cytochrome P450 2B4

Journal of Inorganic Biochemistry, 2004
The interactions between the hemoprotein cytochrome P450 2B4 (CYP 2B4) and riboflavin - a low molecular weight component of the flavoprotein NADPH-dependent cytochrome P450 reductase - were investigated by fluorescence spectroscopy. Riboflavin fluorescence quenching by cytochrome P450 2B4 was used to probe the ligand-enzyme binding (lambda(ex)=385 nm ...
Victoria V, Shumyantseva, Tatiana V, Bulko, Natalia A, Petushkova, Natalia F, Samenkova, Galina P, Kuznetsova, Alexander I, Archakov   +5 more
openaire   +2 more sources

Probe-dependence of competitive fluorescent ligand binding assays to odorant-binding proteins

Analytical and Bioanalytical Chemistry, 2019
Ligand binding experiments between small chemicals and proteins and the evaluation of dissociation constants of their complexes in competitive binding assays often rely on displacement of reporter probes by the tested ligand. The most widely adopted protocol uses a fluorescent ligand which changes its emission spectrum when bound to a protein.
Jiajun, Tan, Valeriia, Zaremska, Sierin, Lim, Wolfgang, Knoll, Paolo, Pelosi   +4 more
openaire   +2 more sources

Development of a fluorescence polarization binding assay for asialoglycoprotein receptor

Analytical Biochemistry, 2012
Asialoglycoprotein receptor (ASGP-R) has been actively investigated for targeted delivery of therapeutic agents into hepatocytes because this receptor is selectively and highly expressed in liver and has a high internalization rate. Synthetic cluster glycopeptides (e.g., triGalNAc) bind with high affinity to ASGP-R and, when conjugated to a therapeutic
Anna Y, Kornilova, Bethany, Algayer, Michael, Breslin, Victor, Uebele   +3 more
openaire   +2 more sources

General Label-Free Fluorescent Aptamer Binding Assay Using Cationic Conjugated Polymers

Analytical Chemistry, 2022
With more and more new aptamers being reported, a general, cost-effective yet reliable aptamer binding assay is still needed. Herein, we studied cationic conjugated polymer (CCP)-based binding assays taking advantage of the conformational change of aptamer after binding with a target, which is reflected by the fluorescence change of the CCP. Poly(3-(3'-
Pengbo Zhang, Ke Qin, Anand Lopez, Zhengping Li, Juewen Liu   +4 more
openaire   +2 more sources

A fluorescence-based detergent binding assay for protein hydrophobicity

Analytical Biochemistry, 1986
Protein hydrophobicity is often detected by binding of protein to micelles of a mild detergent. A new fluorescence method for detection of this binding is presented. The method is based on a long-range quenching of tryptophan fluorescence by energy transfer to a pyrene-labeled phospholipid probe incorporated into micelles of Brij 96.
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Development of a fluorescence polarization binding assay for folate receptor

Analytical Biochemistry, 2013
Folate receptor (FR) has been actively investigated for targeted delivery of therapeutics into cancer cells because this receptor is selectively and highly expressed in carcinomas. Because FR rapidly cycles between the cell surface and cytoplasm, folic acid conjugated to a therapeutic agent can drive targeted therapeutic delivery to cancer cells.
Anna Y, Kornilova, Bethany, Algayer, Michael, Breslin, George H, Addona, Victor, Uebele   +4 more
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A Fluorescence‐Lifetime‐Based Binding Assay for Class IIa Histone Deacetylases

Chemistry – A European Journal, 2017
AbstractClass IIa histone deacetylases (HDACs) show extremely low enzymatic activity and no commonly accepted endogenous substrate is known today. Increasing evidence suggests that these enzymes exert their effect rather through molecular recognition of acetylated proteins and recruiting other proteins like HDAC3 to the desired target location ...
Christian Meyners, Monique Mertens, Pablo Wessig, Franz‐Josef Meyer‐Almes   +3 more
openaire   +3 more sources

Fluorescence-Based Binding Assay for Screening Ligands of Angiotensin Receptors

2017
Binding assay is a common technique used to characterize ability of a ligand to interact with a specific biological target. A number of parameters, such as binding affinity, receptor density, and association/dissociation rate constants, can be measured by means of this technique.
Maiia E, Bragina, Nikolaos, Stergiopulos, Rodrigo A, Fraga-Silva   +2 more
openaire   +2 more sources