Results 21 to 30 of about 927,051 (305)

Insight into the binding of a non-toxic, self-assembling aromatic tripeptide with ct-DNA: Spectroscopic and viscositic studies

open access: yesBiochemistry and Biophysics Reports, 2017
The report describes the synthesis, self-association and DNA binding studies of an aromatic tripeptide H-Phe-Phe-Phe-OH (FFF). The peptide backbone adopts β—sheet conformation both in solid and solution.
Soumi Biswas   +7 more
doaj   +1 more source

Functional Analysis of Odorant-Binding Proteins 12 and 17 from Wheat Blossom Midge Sitodiplosis mosellana Géhin (Diptera: Cecidomyiidae)

open access: yesInsects, 2020
The wheat blossom midge Sitodiplosis mosellana, one of the most disastrous wheat pests, depends highly on olfactory cues to track suitable plants. To better understand the olfactory recognition mechanisms involved in host selection, in the present study ...
Weining Cheng   +4 more
doaj   +1 more source

Characterisation of Bombyx mori odorant-binding proteins reveals that a general odorant-binding protein discriminates between sex pheromone components [PDF]

open access: yes, 2009
In many insect species, odorant-binding proteins (OBPs) are thought to be responsible for the transport of pheromones and other semiochemicals across the sensillum lymph to the olfactory receptors (ORs) within the antennal sensilla.
Adams   +79 more
core   +1 more source

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction. [PDF]

open access: yes, 2016
The intrinsic apoptosis pathway occurs through the release of mitochondrial cytochrome c to the cytosol, where it promotes activation of the caspase family of proteases.
Christian, Thomas   +6 more
core   +2 more sources

Heterogeneous transition metal–based fluorescence polarization (HTFP) assay for probing protein interactions

open access: yesBioTechniques, 2009
Analyses of protein interactions are fundamental for the investigation of molecular mechanisms responsible for cellular processes and diseases, as well as for drug discovery in the pharmaceutical industry.
Alexander Riechers   +3 more
doaj   +1 more source

Aptamer-based cocaine assay using a nanohybrid composed of ZnS/Ag2Se quantum dots, graphene oxide and gold nanoparticles as a fluorescent probe [PDF]

open access: yes, 2020
Authors report on a new fluoro-graphene-plasmonic nanohybrid aptamer-based fluorescent nanoprobe for cocaine. To construct the nanoprobe, newly synthesized glutathione-capped ZnS/Ag 2Se quantum dots (QDs) were first conjugated to graphene oxide (GO) to ...
Abdolvand, Amin   +4 more
core   +2 more sources

A Simple Theoretical Model for Fluorescence Polarization Binding Assay Development [PDF]

open access: yesSLAS Discovery, 2006
Fluorescence polarization is a screening technology that is radioactivity free, homogeneous, and ratiometric. The signal measured with this technology is a weighted value of free and bound ligand. As a consequence, saturation curves are accessible only after calculation of the corresponding concentrations of free and bound ligand.
Olivier, Nosjean   +5 more
openaire   +2 more sources

On-chip determination of C-reactive protein using magnetic particles in continuous flow [PDF]

open access: yes, 2014
We demonstrate the application of a multilaminar flow platform, in which functionalized magnetic particles are deflected through alternating laminar flow streams of reagents and washing solutions via an external magnet, for the rapid detection of the ...
Greenman, John   +4 more
core   +1 more source

Tryptophan Fluorescence Quenching Assays for Measuring Protein-ligand Binding Affinities: Principles and a Practical Guide

open access: yesBio-Protocol, 2019
Tryptophan fluorescence quenching is a type of fluorescence spectroscopy used for binding assays. The assay relies on the ability to quench the intrinsic fluorescence of tryptophan residues within a protein that results from changes in the local ...
Anthony Yammine, Jinlong Gao, Ann Kwan
doaj   +1 more source

Two-Photon Excitation in Fluorescence Polarization Receptor-Ligand Binding Assay [PDF]

open access: yesSLAS Discovery, 2005
Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence.
Marko E, Tirri   +5 more
openaire   +2 more sources

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