Results 31 to 40 of about 927,051 (305)
A blue light receptor that mediates RNA binding and translational regulation [PDF]
, 2019 Sensory photoreceptor proteins underpin light-dependent adaptations in nature and enable the optogenetic control of organismal behavior and physiology.
Bittl, Robert +9 more
core +1 more source
Sensors, 2009 Fluorescence enhancement of fluorescein isothiocyanate-labeled protein A (FITC-protein A) caused by the binding with immunoglobulin G (IgG) in bovine plasma was studied. FITC-protein A was immobilized onto a glass surface by covalent bonds.
Kiyotaka Sakai +3 more
doaj +1 more source
LRRK2RCKW Widefield fluorescence microtubule binding assay v1
, 2022 This assay uses TMR labeled LRRK2 or LRRK1 RCKW to measure binding to microtubules in vitro. Created by David Snead. Adapted to protocols.io by Mariusz Matyszewski. As used in Snead, Matyszewski, Dickey et al. 2022.
openaire +1 more source
Mechanism of DNA Recognition at a Viral Replication Origin [PDF]
, 2006 Recognition of the DNA origin by the Epstein-Barr nuclear antigen 1 (EBNA1) protein is the primary event in latentphase genome replication of the Epstein-Barr virus, a model for replication initiation in eukaryotes.
de Prat Gay, Gonzalo +3 more
core +1 more source
Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. [PDF]
, 2020 Anti-CRISPRs (Acrs) are small proteins that inhibit the RNA-guided DNA targeting activity of CRISPR-Cas enzymes. Encoded by bacteriophage and phage-derived bacterial genes, Acrs prevent CRISPR-mediated inhibition of phage infection and can also block ...
Doudna, Jennifer A +5 more
core +3 more sources
The crystal structure of Pneumolysin at 2.0 Å resolution reveals the molecular packing of the pre-pore complex [PDF]
, 2015 Pneumolysin is a cholesterol-dependent cytolysin (CDC) and virulence factor of Streptococcus pneumoniae. It kills cells by forming pores assembled from oligomeric rings in cholesterol-containing membranes.
Andrew, Peter W +8 more
core +1 more source
Fluorescent Receptor Binding Assay for Detecting Ciguatoxins in Fish
PLOS ONE, 2016 Ciguatera fish poisoning is an illness suffered by > 50,000 people yearly after consumption of fish containing ciguatoxins (CTXs). One of the current methodologies to detect ciguatoxins in fish is a radiolabeled receptor binding assay (RBA(R)). However, the license requirements and regulations pertaining to radioisotope utilization can limit the ...
Hardison, D. Ransom +11 more
openaire +5 more sources
Peptidoglycan glycosyltransferase-ligand binding assay based on tryptophan fluorescence quenching [PDF]
Biochimie, 2018 Peptidoglycan glycosyltransferases (GTase) of family 51 are essential enzymes for the synthesis of the glycan chains of the bacterial cell wall. They are considered potential antibacterial target, but discovery of inhibitors was hampered so far by the lack of efficient and affordable screening assay. Here we used Staphylococcus aureus MtgA to introduce
Dahmane, Ismahene +5 more
openaire +2 more sources
Nanostructured luminescently labeled nucleic acids [PDF]
, 2017 Important and emerging trends at the interface of luminescence, nucleic acids and nanotechnology are: (i) the conventional luminescence labeling of nucleic acid nanostructures (e.g. DNA tetrahedron); (ii) the labeling of bulk nucleic acids (e.g. single‐
Adleman +105 more
core +1 more source
A Fluorescence Lifetime–Based Binding Assay to Characterize Kinase Inhibitors [PDF]
SLAS Discovery, 2007 The authors present a fluorescence lifetime-based kinase binding assay that identifies and characterizes compounds that bind to the adenosine triphosphate (ATP)-binding pocket of a range of tyrosine and serine/threonine kinases. The assay is based on displacement of an Alexa Fluor 647 conjugate of staurosporine from the ATP-binding site of a kinase ...
Connie S, Lebakken +2 more
openaire +2 more sources