Results 61 to 70 of about 927,051 (305)

Synthesis and characterization of a functional benzoylecgonine – bovine serum albumin conjugate for quantification of a humanized monoclonal anti-cocaine antibody

Biochemistry and Biophysics Reports
We have developed a humanized monoclonal anti-cocaine antibody (h2E2), as a potential solution for cocaine use disorder. A key milestone was the development of an assay to quantify this monoclonal antibody (mAb) in animal and human blood.
Rose P. Webster, Terence L. Kirley, Andrew R. Ray, Zhen-Hin Chan, Andrew B. Norman   +4 more
doaj   +1 more source

Development of a Competition-Binding Assay to Determine Binding Affinity of Molecules to Neuromelanin via Fluorescence Spectroscopy

Biomolecules, 2019
Neuromelanin, the polymeric form of dopamine which accumulates in aging neuronal tissue, is increasingly recognized as a functional and critical component of a healthy and active adult human brain.
Jackson Fink, Heather Pathak, John Smith, Cindy Achat-Mendes, Robert L. Haining   +4 more
doaj   +1 more source

Spatial Expression Analysis of Odorant Binding Proteins in Both Sexes of the Aphid Parasitoid Aphidius gifuensis and Their Ligand Binding Properties

Frontiers in Physiology, 2022
In China, Aphidius gifuensis is one of the most common endoparasitoids of the green peach aphid Myzus persicae and grain aphid Sitobion miscanthi in the field.
Xin Jiang, Xin Jiang, Yaoguo Qin, Yaoguo Qin, Jun Jiang, Yun Xu, Frédéric Francis, Jia Fan, Julian Chen   +8 more
doaj   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Characterization of the Adherence of Clostridium difficile Spores: The Integrity of the Outermost Layer Affects Adherence Properties of Spores of the Epidemic Strain R20291 to Components of the Intestinal Mucosa [PDF]

, 2016
Indexación: Web of Science.Clostridium difficile is the causative agent of the most frequently reported nosocomial diarrhea worldwide. The high incidence of recurrent infection is the main clinical challenge of C. difficile infections (CBI). Formation of
Banawas, Saeed, Calderón, Iván, Castro-Córdova, Pablo, Fuentes, Juan A., Gil, Fernando, Miranda-Cárdenas, Camila, Mora-Uribe, Paola, Paredes-Sabja, Daniel, Rodas, Paula I., Sarker, Mahfuzur R.   +9 more
core   +2 more sources

Quantitation of lysolipids, fatty acids, and phospholipase A2 activity and correlation with membrane polarity

Journal of Lipid Research, 2012
Acrylodan-labeled rat-intestinal fatty acid binding protein, ADIFAB, binds both of lysophosphatidylcholines (LPC) and FA. Binding displaces Acrylodan and its fluorescence peak shifts from 432 to 505 nm.
Jasmeet Singh, Radha Ranganathan
doaj   +1 more source

Screening for oligonucleotide binding affinity by a convenient fluorescence competition assay [PDF]

Nucleic Acids Research, 1999
A competitive homogeneous quenched fluorescence assay system is described for the high throughput screening of DNA conjugates that bind to single-stranded DNA. Fluorescence signal is generated by competitive binding of the sample molecule to a target strand labelled with a quencher probe, which is otherwise hybridised to a complementary strand ...
J G, Harrison, X, Liu, S, Balasubramanian   +2 more
openaire   +3 more sources

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Cellular Models of Aggregation-Dependent Template-Directed Proteolysis to Characterize Tau Aggregation Inhibitors for Treatment of Alzheimer's Disease [PDF]

, 2015
Copyright © 2015, The American Society for Biochemistry and Molecular Biology. Acknowledgements-We thank Drs Timo Rager and Rolf Hilfiker (Solvias, Switzerland) for polymorph analyses.Peer reviewedPublisher ...
Clunas, Scott, Harrington, Charles R, Harrington, Kathleen A, Horsley, David, Ishaq, Ahtsham, Kemp, Steven J, Larch, Christopher P, Marshall, Colin, Nicoll, Sarah L, Rickard, Janet E, Simpson, Michael, Sinclair, James P, Storey, John M D, Storey, Lynda J, Wischik, Claude M   +14 more
core   +1 more source

Applications of Graphene Quantum Dots in Biomedical Sensors [PDF]

, 2020
Due to the proliferative cancer rates, cardiovascular diseases, neurodegenerative disorders, autoimmune diseases and a plethora of infections across the globe, it is essential to introduce strategies that can rapidly and specifically detect the ultralow ...
Altintas, Zeynep, Mansuriya, Bhargav D.
core   +1 more source