Results 101 to 110 of about 77,532 (261)

Real-time wide-field fluorescence lifetime imaging via single-snapshot acquisition for biomedical applications

PhotoniX
Fluorescence lifetime imaging (FLI) is a powerful tool for investigating molecular processes, microenvironmental parameters, and molecular interactions across tissue to (sub-)cellular levels.
Vikas Pandey, Euan Millar, Ismail Erbas, Luis Chavez, Jack Radford, Isaiah Crosbourne, Mansa Madhusudan, Gregor G. Taylor, Nanxue Yuan, Claudio Bruschini, Stefan T. Radev, Margarida M. Barroso, Andrew Tobin, Xavier Michalet, Edoardo Charbon, Daniele Faccio, Xavier Intes   +16 more
doaj   +1 more source

A fluorescence lifetime-based novel method for accurate lipid quantification of BODIPY vital-stained C. elegans

Journal of Lipid Research
Lipid droplets (LDs) are organelles associated with lipid storage and energy metabolism, thus, their morphology and quantity are of significant research interest.
Chen Xu, Jintao Luo, Yong Yu
doaj   +1 more source

Mapping Correlated Environmental Landscapes in the Fluorescence Response of a Pyrenylated Charge‐Transfer Probe via Data‐Driven Nonlinear Regression

Advanced Materials Interfaces, EarlyView.
A pyrenylated donor–acceptor probe exhibits environment‐dependent fluorescence governed by intramolecular charge transfer, restricted molecular motion, and supramolecular organization. Nonlinear regression accurately reconstructs fluorescence responses to viscosity, temperature, concentration, and pH, while correlated parameter maps reveal hidden ...
Harshal V. Barkale, Anakhi Hazarika, Nilanjan Dey   +2 more
wiley   +1 more source

Large‐Scale and Cost‐Effective Fabrication of Ultra‐Thin, Biodegradable Microelectrode Arrays and Pressure Sensors Using Laser Micromachining

Advanced Materials Technologies, EarlyView.
A mask‐free and cost‐effective UV‐pico‐second laser‐based microfabrication method is proposed to fabricate large‐area biodegradable microelectrode arrays and pressure sensors. These devices demonstrate low impedance, good conformability, excellent biocompatibility, and rapid degradation, providing a new route for next‐generation transient electronics ...
Bhavani Prasad Yalagala, Asfand Tanwear, María Cerezo‐Sánchez, Hossein Parvizi, Morteza Amjadi, Hadi Heidari   +5 more
wiley   +1 more source

3D‐Printed Corneal Substitutes: Materials, Fabrication, and Preclinical Progress

Advanced Materials Technologies, EarlyView.
Successful clinical translation of 3D‐printed corneal substitutes relies on the interplay between the bioink properties, cellular component, and the fabrication process. These factors influence the critical properties of the construct, including optical transparency, mechanical stability, suture retention, that ultimately govern long‐term stromal ...
Shadi Moshayedi, Ashley Vazquez, Alexander J. Choi, Dimitrios Karamichos, Jayoung Kim   +4 more
wiley   +1 more source

3D Printed Photon Shield Assembly for Low‐Noise Visual Stimulation During Two‐Photon Imaging in Mice

Advanced Materials Technologies, EarlyView.
We present the Photon Shield, an open‐hardware system for low‐noise two‐photon calcium imaging during visual stimulation in mice. Combining a 3D‐printed headplate and rail‐mounted shield substantially reduces stray‐light contamination, as quantitatively verified. Functional validation confirms reliable cortical responses. Open design files enable broad
Gergely Katona, Andrea Slézia, András Dávid, Ábel Petik, Dániel Hillier, István Ulbert, Attila Kaszás   +6 more
wiley   +1 more source

3D convolutional neural networks predict cellular metabolic pathway use from fluorescence lifetime decay data

APL Bioengineering
Fluorescence lifetime imaging of the co-enzyme reduced nicotinamide adenine dinucleotide (NADH) offers a label-free approach for detecting cellular metabolic perturbations.
Linghao Hu, Daniela De Hoyos, Yuanjiu Lei, A. Phillip West, Alex J. Walsh   +4 more
doaj   +1 more source

Photoluminescence Reshaping via Structural Confinement and Local Density of Optical States Control in Ir(III)‐Doped Biodegradable Metasurfaces

Advanced Materials Technologies, EarlyView.
Phosphorescent biodegradable metasurface showing vibronic selection and spectral photoluminescence narrowing. Anti‐correlation between experimental LDOS and measured lifetimes. ABSTRACT Metasurfaces incorporating phosphorescent emitters offer a route to engineer radiative and vibronic processes beyond chemical design.
Vincenzo Caligiuri, Andrea Smeriglio, Svetlana Siprova, Olga Favale, Francesca Leone, Roberto Termine, Debora Policastro, Alessandra Crispini, Attilio Golemme, Nicolas Godbert, Iolinda Aiello, Antonio De Luca   +11 more
wiley   +1 more source

Recent Advances in Transparent Microelectrode Device Architecture and Fabrication Strategies for Biomedical Applications

Advanced Materials Technologies, EarlyView.
Transparent microelectrode arrays enable simultaneous optical and electrical electrophysiology with high spatiotemporal resolution, allowing multimodal observations of dynamic biological systems. Advances in materials and device architectures improve device performance by addressing key trade‐offs between optical transparency, impedance, and ...
Michael Abraham Listyawan, Tran Bach Dang, Chi Cong Nguyen, Yee Lap Pong, Tianruo Guo, Thanh Nho Do, Hoang‐Phuong Phan   +6 more
wiley   +1 more source

Monitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy

Theranostics, 2012
Photodynamic Therapy (PDT) provides an opportunity for treatment of various invasive tumors by the use of a cancer targeting photosensitizing agent and light of specific wavelengths. However, real-time monitoring of drug localization is desirable because
Shu-Chi Allison Yeh, Kevin R. Diamond, Michael S. Patterson, Zhaojun Nie, Joseph E. Hayward, Qiyin Fang
doaj