Results 81 to 90 of about 207,530 (298)

Hadamard-transform fluorescence-lifetime imaging

Optics Express, 2016
We discuss a Hadamard-transform-based fluorescence-lifetime-imaging (HT-FLI) technique for fluorescence-lifetime-imaging microscopy (FLIM). The HT-FLI uses a Fourier-transform phase-modulation fluorometer (FT-PMF) for fluorescence-lifetime measurements, where the modulation frequency of the excitation light is swept linearly in frequency from zero to a
Mizuno, Takahiko, Iwata, Tetsuo
openaire   +2 more sources

Photon Avalanching Nanoparticles: The Next Generation of Upconverting Nanomaterials?

Advanced Functional Materials, EarlyView.
This Perspective outlines the mechanistic foundations that enable photon‐avalanche (PA) behavior in lanthanide nanomaterials and contrasts them with emerging application spaces and forward‐looking design strategies. By bridging threshold engineering, energy‐transfer dynamics, and materials engineering, we provide a coherent roadmap for advancing the ...
Kimoon Lee, Yunseo Kim, Hyeong‐ku Jo, Dongjoon Yoon, Byeonghyeok Im, WonJeong Yu, Eui‐Rim On, Changhwan Lee   +7 more
wiley   +1 more source

Fluorescence Lifetime Imaging of Apoptosis

Tomography, 2015
Genetically encoded fluorescence resonance energy transfer (FRET) reporters are powerful tools for analyzing cell signaling and function at single-cell resolution in standard 2D cell cultures, but these reporters rarely have been applied to 3D environments.
Xiao, Annie, Gibbons, Anne E., Luker, Kathryn E., Luker, Gary D.   +3 more
openaire   +2 more sources

Disordered Carbon Shells Enable Shape Dependent Fluorescence Enhancement in Nanodiamond Quantum Sensors Revealed by Correlative TEM–Fluorescence Microscopy

Advanced Functional Materials, EarlyView.
Molten KNO2 treatment induces a lowrefractive index disordered carbon shell on fluorescent nanodiamonds, enhancing fluorescence emission while preserving spin coherence. Machine learningassisted correlative TEMPL enables direct single‐particle resolution of this enhancement relative to air‐oxidized nanodiamonds of similar morphology, establishing a ...
Parkarsh Kumar, Tom Vaillant, Mehran Kianinia, Haotian Wen, Adam Stewart, Peter J. Reece, Hugues A. Girard, Jean‐Charles Arnault, Huan‐Cheng Chang, Igor Aharonovich, Shery L. Y. Chang   +10 more
wiley   +1 more source

Fluorescence decay data analysis correcting for detector pulse pile-up at very high count rates

, 2017
Using Time-Correlated Single Photon Counting (TCSPC) for the purpose of fluorescence lifetime measurements is usually limited in speed due to pile-up.
Dowler, Rhys, Koenig, Marcelle, Patting, Matthias, Reisch, Paja, Sackrow, Marcus, Wahl, Michael   +5 more
core   +1 more source

Spectroscopic characterization of reaction centers of the (M)Y210W mutant of the photosynthetic bacterium Rhodobacter sphaeroides [PDF]

, 1994
The tyrosine-(M)210 of the reaction center of Rhodobacter sphaeroides 2.4.1 has been changed to a tryptophan using site-directed mutagenesis. The reaction center of this mutant has been characterized by low-temperature absorption and fluorescence ...
Arlt, Thomas, Francke, Christof, Gast, Peter, Hoff, Arnold J., Noort, Paula I. van, Otte, Stephan C. M., Schelvis, Hans P. M., Schmidt, Stefan, Shochat, Susana, Vijgenboom, Erik, Zinth, Wolfgang   +10 more
core   +1 more source

DNA‐Origami‐Assembled Rhodium Nanoantennas for Deep‐UV Label‐Free Single‐Protein Detection

Advanced Functional Materials, EarlyView.
Deep‐UV autofluorescence of single proteins in DNA origami‐based rhodium nanoantennas. Precisely positioned within the plasmonic nanogap, proteins retain their native state while their intrinsic emission is strongly amplified, enabling label‐free single‐molecule detection and opening new opportunities for highly sensitive and specific biosensing ...
Nicco Corduri, Malavika Kayyil Veedu, Yifan Yu, Yanqiu Zou, Jie Liu, Denis Garoli, Guillermo P. Acuna, Jérôme Wenger, Karol Kołątaj   +8 more
wiley   +1 more source

Enhancing STED microscopy via fluorescence lifetime unmixing and filtering in two-species SPLIT-STED

Methods in Microscopy
Simultaneous super-resolution imaging of multiple fluorophores remains a major challenge in STimulated Emission Depletion (STED) microscopy due to spectral overlap of STED-compatible fluorophores.
Deschênes Andréanne, Ollier Antoine, Lafontaine Marie, Michaud-Gagnon Albert, Santiague Jeffrey-Gabriel Steavan, Bilodeau Anthony, Gagné Christian, De Koninck Paul, Lavoie-Cardinal Flavie   +8 more
doaj   +1 more source

ANS fluorescence: Potential to discriminate hydrophobic sites of proteins in solid states

Biochemistry and Biophysics Reports, 2020
In the current study, ANS fluorescence was established as a powerful tool to study proteins in solid-state. Silk fibroin from Bombyx mori cocoons was used as a paradigm protein.
Aytaj J. Guliyeva, Oktay K. Gasymov
doaj   +1 more source

Ultra-portable explosives sensor based on a CMOS fluorescence lifetime analysis micro-system [PDF]

, 2011
The paper published in AIP Advances (a new journal from the American Institute of Physics) had 1100 article downloads in the first 5 months after publication, and was on the journal’s “most read” list for 4 months.
Dawson, Martin D., Gong, Zheng, Gu, Erdan, Henderson, Robert K., Mckendry, Jonathan, Rae, Bruce R., Samuel, Ifor D. W., Turnbull, Graham A., Wang, Yue   +8 more
core   +2 more sources