Results 191 to 200 of about 207,530 (298)

Material‐Induced Nuclear Deformation Controls Chromatin Architecture in Adipose Stem Cells

Advanced Science, EarlyView.
Tuning cell and cytoskeleton mechanics modulated nuclear shape and heterochromatin organization in ASCs. Distinct cytoskeletal architectures induced nuclear morphologies from oblate to prolate ellipsoids. Large elongated cells with a structured actin cap exhibited high nuclear strain, driving nuclear envelope deformation and heterochromatin ...
Carlo F. Natale, Luca Messina, Valeria Panzetta, Stefania Saporito, Costantino Menna, Maurizio Ventre, Paolo A. Netti   +6 more
wiley   +1 more source

Visualizing intracellular milieu and electric field-induced cell responses by fluorescence lifetime imaging microscopy. [PDF]

Sci Rep
Awasthi K, Huang WC, Hsu HY, Ohta N.
europepmc   +1 more source

Timing Mechanotransduction: Mechanically Dynamic Biomaterials Reveal the Temporal Hierarchy of YAP/TAZ Control Nodes

Advanced Science, EarlyView.
This work develops dynamically softening polyacrylamide hydrogels for time‐resolved imaging during continuous mechanical transitions. The study revealed that mechanotransduction is biphasic; YAP/TAZ inactivation is driven by early loss of the nucleocytoskeletal continuum connecting subnuclear adhesions, F‐actin, and the nuclear envelope, coupled with ...
Alessandro Gandin, Giada Vanni, Veronica Torresan, Margherita Pelosin, Rebecca Busetto, Anna Citron, Ambela Suli, Paolo Contessotto, Carlo Albanese, Francesca Zanconato, Tito Panciera, Stefano Piccolo, Giovanna Brusatin   +12 more
wiley   +1 more source

Fluorescence Lifetime Imaging Application to Probe GTPase Activation in Macrophage Cell Line, Using the Time-Domain FastFLIM Modality. [PDF]

Methods Mol Biol
Miskolci V, de Assis Lima M, Cox D, Hodgson L.   +3 more
europepmc   +1 more source

Restriction of Individual Branched‐Chain Amino Acids has Distinct Effects on the Development and Progression of Alzheimer's Disease in 3xTg Mice

Advanced Science, EarlyView.
Protein restriction (PR) slows Alzheimer's disease (AD) in mice, and other benefits of PR are due to decreased branched‐chain amino acids (BCAAs). We show that restricting any BCAA has benefits, with sex‐ and BCAA‐specific impacts on pathology, molecular signaling, and cognition.
Reji Babygirija, Cara L. Green, Michelle M. Sonsalla, Izabelle Marie F. Le, Fan Xiao, Sarah Yandell, Mariah F. Calubag, Michaela E. Trautman, Anna Tobon, Ryan Matoska, Chung‐Yang Yeh, Charles I. Opara, Isaac Grunow, Diana Vertein, Sophia Schlorf, Bailey A. Knopf, Michael J. Rigby, David A. Harris, Mark P. Keller, Alan D. Attie, Luigi Puglielli, Cholsoon Jang, Dudley W. Lamming   +22 more
wiley   +1 more source

Hepatocyte PIEZO1 Negatively Regulates Lipogenesis and Ameliorates MASLD by Sensing Membrane Tension and Activating AMPK

Advanced Science, EarlyView.
Hepatocytes are subjected to increased membrane tension along with lipid accumulation. Loss of PIEZO1 exacerbates high‐fat diet‐induced MASLD in mice. Activation of PIEZO1 alleviates high‐fat diet‐induced MASLD in mice. PIEZO1 negatively regulates de novo lipogenesis through activation of CaMKK2‐AMPK pathway. ABSTRACT Liver is a central organ for lipid
Hui Chen, Qimeng Wang, Ke Yang, Qinghai Lian, Xuyun Peng, Zhiyong Gong, Xinyi Zhao, Yong Wu, Tian Tao, Siqi Xu, Yifan Chen, Xiaoyan Tang, Jinghui Guo, Geyang Xu, Qi Zhang   +14 more
wiley   +1 more source

Label-free fluorescence lifetime imaging for rapid discrimination of high-grade prostate cancer in fresh biopsy cores: a feasibility study. [PDF]

J Biomed Opt
Bec J, Zhou X, Tipirneni Y, Chen S, Qi J, Iczkowski KA, Dall'Era M, Marcu L.   +7 more
europepmc   +1 more source

Embedded CRISPRi Enhances Gene‐Silencing Efficiency in Drosophila

Advanced Science, EarlyView.
Current CRISPR interference (CRISPRi) technology in Drosophila has limited efficiency. This study introduces the emCRISPRi platform, which significantly enhances transcriptional silencing efficacy by embedding inhibitory domains within the dCas9 architecture.
Pengchong Fu, Xuedi Zhang, Ying Zhou, Jie Zheng, Angyang Sun, Kailong Zhuang, Weiwei Bao, Guanjun Gao   +7 more
wiley   +1 more source

Adaptive fluorescence lifetime imaging with per-pixel signal optimization and flexible scanning. [PDF]

Biomed Opt Express
Xie S, Williams GOS, Akram AR, Erdogan AT, Hopgood JR.   +4 more
europepmc   +1 more source

A Tac1‐Expressing Brainstem Pathway Underlies the Pathogenesis of Trigeminal Neuralgia

Advanced Science, EarlyView.
A critical TG‐Sp5CTac1‐PBNTac1 pathway drives trigeminal neuropathic pain (TNP). Tac1‐expressing parabrachial nucleus (PBNTac1) neurons exhibit heightened responses to innocuous stimuli in TNP, and chemogenetic inhibition of these neurons effectively prevents TNP development.
Liting Sun, Jia‐Jia Wang, Xiang‐Yu Li, Xin‐Yi Lin, Juan Li, Qiu‐Tong Yu, Zi‐Han Wang, Xue‐Ping Gao, Lei Jin, Wei‐Ke Li, Tian‐Lin Cheng, Juan Deng   +11 more
wiley   +1 more source