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Fluorescence lifetime imaging ophthalmoscopy

Progress in Retinal and Eye Research, 2017
Imaging techniques based on retinal autofluorescence have found broad applications in ophthalmology because they are extremely sensitive and noninvasive. Conventional fundus autofluorescence imaging measures fluorescence intensity of endogenous retinal fluorophores.
Martin Hammer, Mikhail Y Berezin, Chantal Dysli   +2 more
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Video rate fluorescence lifetime imaging and fluorescence lifetime endoscopy

SPIE Proceedings, 2005
We report real-time (video-rate) fluorescence lifetime imaging and its application to tissue autofluorescence and endoscopy, demonstrating FLIM of unstained ex vivo tissue at update rates of 5.5 Hz through a flexible endoscope.
James McGinty, Ian Munro, Neil P. Galletly, Jose Requejo-Isidro, Daniel S. Elson, Christopher Dunsby, Mark A. Neil, Gordon W. Stamp, Paul M. French   +8 more
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Fluorescence Lifetime Imaging Microscopy

Current Protocols in Cell Biology, 2004
AbstractFluorescent lifetime imaging microscopy is a powerful tool to enhance the contrast in images of biological samples and to investigate the local environment of a fluorochrome. FLIM allows the detection of protein‐protein interactions and their biochemical state by the quantitative detection of Förster resonance energy transfer (FRET) between ...
Alessandro, Esposito, Fred S, Wouters
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FLUORESCENCE LIFETIMES OF ANGULAR FUROCOUMARINS

Photochemistry and Photobiology, 1987
AbstractProperties of singlet states of a range of non‐substituted and aceto‐substituted angular furocoumarins as investigated by the technique of time‐resolved fluorescence are reported. In the three solvents studied, water, ethanol and benzene, a variety of 1, 2 or 3 fluorescence lifetime components were found and resolved, the importance of which ...
Andreoni A, CUBEDDU, RINALDO, Knox CN, Truscott TG   +3 more
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Fluorescence Lifetime Correlation Spectroscopy

Journal of Fluorescence, 2006
This article explains the basic principles of FLCS, a genuine fusion of Time-Correlated Single Photon Counting (TCSPC) and Fluorescence Correlation Spectroscopy (FCS), using common terms and minimum mathematics. The usefulness of the method is demonstrated on simple FCS experiments.
Peter, Kapusta, Michael, Wahl, Ales, Benda, Martin, Hof, Jörg, Enderlein   +4 more
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Fluorescence lifetime tomography

Optical Systems for Mechanical and Biophysical Investigation
Time-resolved fluorescence tomography enables precise spatial differentiation of fluorophores with overlapping spectra. Using a scanning system, we acquired decay kinetics from a sample phantom filled with acridine orange and fluorescein, revealing distinct lifetime contrasts.
Fabian Rieder, Karl Otto Dirk Schnelle, Jan Laufer, Franz-Josef Schmitt   +3 more
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Fluorescence lifetimes of Chlorella pyrenoidosa

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1976
The flucrescence decay of Chlorella pyrenoidosa has been investigated under a variety of conditions in the picosecond and nanosecond time regions. Most of the fluorescence is accounted for by an expression of the form I(t) = I0exp-(At+Bt1/2) though an additional exponential term is required to include a weak component of lifetime 32 ps observable only ...
L, Harris, G, Porter, J A, Synowiec, C J, Tredwell, J, Barber   +4 more
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Fluorescence lifetime-resolved imaging

Photosynthesis Research, 2009
This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint readers who are not experts with the basic methods for measuring lifetime-resolved signals throughout an image. We present the early FLI (fluorescence lifetime imaging) history, review shortly the instrumentation and experimental design, discuss briefly the ...
Yi-Chun, Chen, Robert M, Clegg
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Fluorescence Lifetime Imaging Microscopy

2007
Publisher Summary Fluorescence lifetime imaging microscopy (FLIM) produces spatially resolved images of fluorophore lifetime (the property describing how rapidly fluorescence decays), providing another dimension of information for visualizing fluorophores and an additional source of contrast.
Ching-Wei, Chang, Dhruv, Sud, Mary-Ann, Mycek   +2 more
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Fluorescence lifetime based bioassays

Methods and Applications in Fluorescence, 2017
Abstract Fluorescence lifetime (FLT) is a robust intrinsic property and material constant of fluorescent matter. Measuring this important physical indicator has evolved from a laboratory curiosity to a powerful and established technique for a variety of applications in drug discovery, medical diagnostics and basic
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