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Fluorescence lifetimes: fundamentals and interpretations
Photosynthesis Research, 2009Fluorescence measurements have been an established mainstay of photosynthesis experiments for many decades. Because in the photosynthesis literature the basics of excited states and their fates are not usually described, we have presented here an easily understandable text for biology students in the style of a chapter in a text book. In this review we
Ulai, Noomnarm, Robert M, Clegg
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Fluorescence lifetime imaging of coral fluorescent proteins
Microscopy Research and Technique, 2007AbstractCorals, like many other coelenterates, contain fluorescent pigments that show considerable homology with the well known green fluorescent protein of the jellyfish Aequoria. In corals, unlike jellyfish, multiple proteins are present and the range of excitations and emissions suggest the possibility of energy transfer.
Guy, Cox, Mikhail, Matz, Anya, Salih
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Confocal Fluorescence Lifetime Imaging
1996The optical microscope, arguably the most mature of all laboratory instruments, is currently enjoying a period of intense development that is opening up many new application areas. These developments have been made possible by the use of new optical arrangements, sophisticated light sources and detectors and by the application of novel fluorescence ...
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2006
A newly developed fluorescence lifetime imaging microscopy (FLIM) system has combined the high-temporal resolution of a streak camera with the high-spatial resolution of a microscope to obtain a two-dimensional distribution of fluorescence lifetimes within living cells. The temporal resolution is as short as 20 ps.
Masanobu, Fujiwara, William, Cieslik
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A newly developed fluorescence lifetime imaging microscopy (FLIM) system has combined the high-temporal resolution of a streak camera with the high-spatial resolution of a microscope to obtain a two-dimensional distribution of fluorescence lifetimes within living cells. The temporal resolution is as short as 20 ps.
Masanobu, Fujiwara, William, Cieslik
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2014
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is largely independent of the local fluorophore concentration and excitation ...
Suhling, Klaus +11 more
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Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is largely independent of the local fluorophore concentration and excitation ...
Suhling, Klaus +11 more
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FLUORESCENCE LIFETIME OF PHYCOERYTHRIN*
Photochemistry and Photobiology, 1968F, Macdowall, M, Walker
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Measurement of Fluorescence Lifetimes
1983The fluorescence lifetime of a substance usually represents the average amount of time the molecule remains in the excited state prior to its return to the ground state. Lifetime measurements are frequently necessary in fluorescence spectroscopy. These data can reveal the frequency of collisional encounters with quenching agents (Chapter 9), the rate ...
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Fluorescence Lifetime Measurements and Biological Imaging
Chemical Reviews, 2010Mikhail Y Berezin
exaly