Results 141 to 150 of about 4,840 (179)

Fluorescence Polarization Competition Immunoassay for Tyrosine Kinases

Methods, 2000
To increase the sensitivity and throughput of protein tyrosine kinase (PTK), simple, homogeneous, nonradioactive, direct and indirect fluorescence polarization (FP) protein tyrosine kinase immunoassays have been developed that are compatible with high-throughput and ultrahigh-throughput screening for developing drugs.
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Fluorescence Polarization Immunoassay for Insulin Preparations

Analytical Letters, 1982
Abstract A fluorescence polarization immunoassay for insulin preparations such as pharmaceutical preparation and solutions after chromatography is described. The assay is rapid, without separation of antibody-bound and free ligands. The minimal amount of insulin detected was 0.6 mU/ tube and the measurable range was from 40 to 600 mU/ml of solution.
Y. Yamaguchi, C. Hayashi, K. Miyai
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Direct fluorescence polarization immunoassay.

Steroids, 1980
Direct fluorescence polarization immunoassay of serum cortisol was established. Non-specific binding of fluorescent-labelled cortisol to serum proteins was successively eliminated by sodium dodecyl sulfate. The minimal amount of cortisol detected was 0.1 ng/tube and serum concentration of 1.0 microgram/dl to 100 microgram/dl of cortisol could be ...
Y, Kobayashi, K, Miyai, N, Tsubota, F, Watanabe   +3 more
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Lead Analysis by Anti-Chelate Fluorescence Polarization Immunoassay

Environmental Science & Technology, 2002
Lead concentrations were determined by a fluorescence polarization immunoassay (FPIA) method that uses polyclonal antibodies raised against the lead(II) chelate of ethylenediamine-N,N,N',N'-tetraacetic acid (EDTA). The technique is based on competition for a fixed concentration of antibody binding sites between Pb-EDTA, formed by treating the sample ...
David K, Johnson, Sherry M, Combs, John D, Parsen, Michael E, Jolley   +3 more
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Long-wavelength fluorescence polarization immunoassay for surfactant determination

Talanta, 2007
A new homogeneous fluoroimmunoassay method based on the use of dynamic long-wavelength fluorescence polarization is presented here for the first time. This methodology, which is applied to the determination of linear alkylbenzenesulfonates (LASs) in water samples, involves the use of a new long-wavelength tracer synthesized from the oxazine dye Nile ...
M L, Sánchez-Martínez, M P, Aguilar-Caballos, S A, Eremin, A, Gómez-Hens   +3 more
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Comparison of a New Serum Topiramate Immunoassay to Fluorescence Polarization Immunoassay

Therapeutic Drug Monitoring, 2010
Topiramate is a newer anticonvulsant used to treat epilepsy, migraines, bipolar disorder, posttraumatic stress, and other conditions. Serum topiramate concentrations are measured to determine optimal levels, address therapeutic failure or drug-drug interactions, and assess compliance.
Christine L H, Snozek, Lisa A, Rollins, Paul W, Peterson, Loralie J, Langman   +3 more
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High-throughput fluorescence polarization immunoassay by using a portable fluorescence polarization imaging analyzer

Lab on a Chip, 2019
A portable fluorescence polarization imaging analyzer was developed for high-throughput fluorescence polarization immunoassay.
Osamu Wakao, Ken Satou, Ayano Nakamura, Polina A. Galkina, Keine Nishiyama, Ken Sumiyoshi, Fumio Kurosawa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Mikhail A. Proskurnin, Koji Shigemura, Akihide Hibara, Manabu Tokeshi   +13 more
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Microchip-based homogeneous immunoassay using fluorescence polarization spectroscopy

Lab Chip, 2009
We have realized fluorescence polarization immunoassay (FPIA) on a microchip in about 1 minute. FPIA is a homogeneous competitive immunoassay which is based on measuring fluorescence polarization after competitive binding of an analyte and a tracer to an antibody. We constructed a microfluidic FPIA system composed of a newly designed microchip, a laser,
Tomoya, Tachi, Noritada, Kaji, Manabu, Tokeshi, Yoshinobu, Baba   +3 more
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[Fluorescence polarization immunoassay and microparticle enzyme immunoassay].

Rinsho byori. The Japanese journal of clinical pathology, 1997
We describe a new clinical laboratory instrument, the Abbott AxSYM, which provides random- and continuous-access testing for immunoassays, 20 onboard reagents, primary tube sampling, and a throughput of 80 to 120 tests per hour. The AxSYM incorporates three separate analytical technologies for processing immunoassays: microparticle enzyme immunoassay ...
M, Suguri, N, Hirose, A, Myoga, R, Doss, N, Tatsumi   +4 more
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A Fluorescence Polarization Competition Immunoassay for Tyrosine Kinases

Analytical Biochemistry, 1998
We have recently reported a homogeneous, nonradioactive fluorescence polarization method to assay protein tyrosine kinase activity. Our original approach can only be used with a peptide substrate and requires large amounts of anti-phosphotyrosine antibody.
R, Seethala, R, Menzel
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