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Stopped-flow Fluorescence Polarization Immunoassay
Combinatorial Chemistry & High Throughput Screening, 2003A general survey of the analytical application of kinetic methodology in fluorescence polarization immunoassay (FPIA) is presented. Stopped-flow mixing technique (SF) allows the initial rate of the immunochemical reaction between the tracer and the antibody to be obtained, which is used as the analytical parameter instead of the equilibrium signal used
Agustina, Gómez-Hens +1 more
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Immunoassay methods based on fluorescence polarization
Talanta, 1989A review on the use of fluorescence polarization in immunoassay procedures is presented. Only the determination of low molecular-weight substances, such as therapeutic agents, drugs of abuse and hormones is considered because the measured change in fluorescence polarization depends on the molecular size of the analyte.
M C, Gutierrez +2 more
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Noncompetitive Fluorescence Polarization Immunoassay for Protein Determination
Analytical Chemistry, 2020Fluorescent polarization immunoassay (FPIA) is a single-step immunoassay method that is applicable to point-of-care testing; however, its applicability to large biomolecules has been restricted because ordinary FPIA is a competitive assay. Here, we report a noncompetitive FPIA using the variable domain from the heavy chain of a camelid antibody (VHH ...
Mao Fukuyama +6 more
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Comparison of a New Serum Topiramate Immunoassay to Fluorescence Polarization Immunoassay
Therapeutic Drug Monitoring, 2010Topiramate is a newer anticonvulsant used to treat epilepsy, migraines, bipolar disorder, posttraumatic stress, and other conditions. Serum topiramate concentrations are measured to determine optimal levels, address therapeutic failure or drug-drug interactions, and assess compliance.
Christine L H, Snozek +3 more
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FLUORESCENT POLARIZATION IMMUNOASSAY OF MICROCYSTIN-LR
Institute of information technology, 2021To control the highly toxic phycotoxin microcystin-LR, which comtaminates food, a test sys-tem based on polarizing fluorescent immunoassay has been developed allowing for rapid screening testing, the detection limit of microcystin-LR is up to 7.5 ng / ml; duration of analy-sis – 10 min.
Olga Dmitrievna Hendrickson +4 more
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Production of Polyclonal Antibodies and Development of Fluorescence Polarization Immunoassay for Sulfanilamide [PDF]
Analytical Letters, 2005 N-sulfanil-4-aminobutyric acid (SAB), which mimics common parts of the sulfonamides' structure, was synthesized and used to produce antibodies to sulfanilamide.
Sergei Eremin, Anatoly Zherdev
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A fluorescence polarization immunoassay for the detection of zearalenone in corn
Analytica Chimica Acta, 2009The aim of this study was to develop a fluorescence polarization immunoassay (FPIA) that is based on the change in fluorescence polarization of fluorescently labeled small antigen when bound by a specific antibody, for use as a screening test for zearalenone (ZEN) in cereals and their products.
Hyang Sook, Chun +4 more
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Fluorescence Polarization Immunoassays for Metal Ions
Combinatorial Chemistry & High Throughput Screening, 2003Antibodies raised against a given metal ion complex of a polyaminopolycarboxylate chelating agent can display specificity for the immunizing chelate and, when used in conjunction with a fluorophore-labeled analog of that chelate, can form the basis for highly sensitive and specific methods for detecting that metal ion by competitive inhibition ...
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Fluorescence polarization immunoassay employing immobilized antibody
Biosensors and Bioelectronics, 1991The use of an antibody immobilized on latex or silver colloid in fluorescence polarization immunoassay (FPI) is assessed. In FPI it is possible to detect antigens of high molecular weight because the molecular weight of the antibody is effectively increased.
M, Tsuruoka, E, Tamiya, I, Karube
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Fluorescence Polarization Immunoassays for the Quantification of Caffeine in Beverages
Journal of Agricultural and Food Chemistry, 2014Homogeneous fluorescence polarization immunoassays (FPIAs) were developed and compared for the determination of caffeine in beverages and cosmetics. FPIAs were performed in cuvettes in a spectrometer for kinetic FP measurements as well as in microtiter plates (MTPs) on a multimode reader.
Lidia, Oberleitner +5 more
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