Results 171 to 180 of about 1,023,890 (203)
Some of the next articles are maybe not open access.
Performance of a fluorescence polarization immunoassay for teicoplanin in serum
Diagnostic Microbiology and Infectious Disease, 1993Teicoplanin is a new glycopeptide antibiotic that is structurally related to vancomycin, but has six major components and is > 90% protein bound. We have developed a competitive homogeneous fluorescence polarization immunoassay (FPIA) for the quantitative determination of teicoplanin in serum.
S H, Mastin, R L, Buck, P A, Mueggler
openaire +2 more sources
Adaptation of fluorescence polarization immunoassay to the assay of macromolecules
Analytical Biochemistry, 1990This paper describes an original methodology for determining macromolecular antigen levels by polarization of fluorescence. it involves the use of fluorescent derivatives of Fab fragments of a monoclonal antibody (Mr 50,000), whose fluorescence polarization rises significantly when it combines with a macromolecular antigen.
P, Urios, N, Cittanova
openaire +2 more sources
Comparison of fluorescence polarization immunoassay and bioassay of vancomycin
Journal of Clinical Microbiology, 1984Human serum samples were analyzed for vancomycin concentrations by two different methods: the fluorescence polarization immunoassay and the disk plate bioassay. Each assay method offered acceptable precision. The correlation between both assay methods was excellent (correlation coefficient = 0.985).
D J, Pohlod +2 more
openaire +2 more sources
Development of a Fluorescence Polarization Immunoassay for Lorazepam Quantification
Therapeutic Drug Monitoring, 1996Lorazepam concentrations have been quantified in biological fluids using gas chromatography and high-performance liquid chromatography (HPLC). However, these methods are too time consuming and labor intensive for most nonresearch laboratories to offer.
P G, Agbuya +4 more
openaire +2 more sources
A Fluorescence Polarization Competition Immunoassay for Tyrosine Kinases
Analytical Biochemistry, 1998We have recently reported a homogeneous, nonradioactive fluorescence polarization method to assay protein tyrosine kinase activity. Our original approach can only be used with a peptide substrate and requires large amounts of anti-phosphotyrosine antibody.
R, Seethala, R, Menzel
openaire +2 more sources
Fluorescence polarization immunoassay. Theory and experimental method
Immunochemistry, 1973Abstract Fluorescence polarization measurements have been applied to diverse immunochemical systems for the purpose of directly following the reaction of fluorescent labeled haptens or antigens with antibody (Dandliker and de Saussure, Immunochemistry, 7, 799 (1970)).
W B, Dandliker +4 more
openaire +2 more sources
Automated Fluorescence Polarization Immunoassay for Monitoring Vancomycin
Therapeutic Drug Monitoring, 1983We have extended fluorescence polarization immunoassay (FPIA) technology for the measurement of drugs to include the complex amphoteric glycopeptide antibiotic vancomycin (molecular weight, 1,449). Fluorescein-labeled vancomycin was employed as a tracer, and antisera specific for vancomycin were raised in rabbits by conventional procedures.
K S, Schwenzer, C H, Wang, J P, Anhalt
openaire +2 more sources
Fluorescence Polarization Competition Immunoassay for Tyrosine Kinases
Methods, 2000To increase the sensitivity and throughput of protein tyrosine kinase (PTK), simple, homogeneous, nonradioactive, direct and indirect fluorescence polarization (FP) protein tyrosine kinase immunoassays have been developed that are compatible with high-throughput and ultrahigh-throughput screening for developing drugs.
openaire +2 more sources
Analytical Biochemistry, 1986
A series of fluorescent disulfonatonaphthalimide derivatives of testosterone and estriol have been synthesized and their fluorescent properties investigated. The fluorescence lifetimes of these derivatives were higher than that of the unreacted fluorescent dye while the quantum yields were of the same order.
B, Desfosses +4 more
openaire +2 more sources
A series of fluorescent disulfonatonaphthalimide derivatives of testosterone and estriol have been synthesized and their fluorescent properties investigated. The fluorescence lifetimes of these derivatives were higher than that of the unreacted fluorescent dye while the quantum yields were of the same order.
B, Desfosses +4 more
openaire +2 more sources
[Fluorescence polarization immunoassay and microparticle enzyme immunoassay].
Rinsho byori. The Japanese journal of clinical pathology, 1997We describe a new clinical laboratory instrument, the Abbott AxSYM, which provides random- and continuous-access testing for immunoassays, 20 onboard reagents, primary tube sampling, and a throughput of 80 to 120 tests per hour. The AxSYM incorporates three separate analytical technologies for processing immunoassays: microparticle enzyme immunoassay ...
M, Suguri +4 more
openaire +1 more source