Results 111 to 120 of about 40,790 (312)

Multiplexed in-gel microfluidic immunoassays: characterizing protein target loss during reprobing of benzophenone-modified hydrogels. [PDF]

, 2019
From whole tissues to single-cell lysate, heterogeneous immunoassays are widely utilized for analysis of protein targets in complex biospecimens. Recently, benzophenone-functionalized hydrogel scaffolds have been used to immobilize target protein for ...
Gopal, Anjali, Herr, Amy E
core   +1 more source

Phase‐Separation of YAP Mediates AJUBA Super Enhancer Activation to Promote Aberrant Mitosis in Breast Cancer

Advanced Science, EarlyView.
YAP undergoes phase separation to activate the AJUBA super‐enhancer, driving aberrant mitosis through spindle checkpoint dysregulation in breast cancer. This study uncovers a mechanistic link between YAP condensates, super‐enhancer activation, and aneuploidy formation.
Rui Zhang, Qingwen Huang, Zhuo Chen, Weijian Meng, Hongliang Dong, Zhihong Qi, Liang Liu, Jie Shen, Daxing Xie   +8 more
wiley   +1 more source

Effect of concentration quenching on fluorescence recovery after photobleaching measurements

Biophysical Journal, 1995
Standard analysis of fluorescence recovery after photobleaching (FRAP) data is valid only if the quantum yield of unphotobleached fluorophores is independent of concentration, yet close molecular packing in two-dimensional systems may lead to significant fluorescence concentration quenching.
Robeson, J.L., Tilton, R.D.
openaire   +2 more sources

Metformin Restores Mitochondrial Function and Neurogenesis in POLG Patient‐Derived Brain Organoids

Advanced Science, EarlyView.
Patient‐derived POLG‐mutant cortical organoids reveal neuronal subtype‐specific mitochondrial and synaptic defects, with dopaminergic neurons most affected. Metformin treatment restores neuronal identity, mitochondrial function, and excitability, increased mtDNA maintenance, and reprogrammed metabolism via TCA and redox pathways.
Zhuoyuan Zhang, Tsering Yangzom, Ning Lu, Shenglong Deng, Xianglu Xiao, Guang Yang, Kristina Xiao Liang   +6 more
wiley   +1 more source

A customized light sheet microscope to measure spatio-temporal protein dynamics in small model organisms.

PLoS ONE, 2015
We describe a customizable and cost-effective light sheet microscopy (LSM) platform for rapid three-dimensional imaging of protein dynamics in small model organisms.
Matthias Rieckher, Ilias Kyparissidis-Kokkinidis, Athanasios Zacharopoulos, Georgios Kourmoulakis, Nektarios Tavernarakis, Jorge Ripoll, Giannis Zacharakis   +6 more
doaj   +1 more source

Studies of intercellular Ca2+ signaling and gap-junction coupling in the developing cochlea of mouse models affected by congenital hearing loss [PDF]

, 2013
Connexin 26 (Cx26) and connexin 30 (Cx30) form gap junction channels that allow the intercellular diffusion of the Ca2+ mobilizing second messenger IP3.
Rodriguez Hernandez, Laura
core   +1 more source

Anomalous transport in the crowded world of biological cells

, 2013
A ubiquitous observation in cell biology is that diffusion of macromolecules and organelles is anomalous, and a description simply based on the conventional diffusion equation with diffusion constants measured in dilute solution fails.
Franosch, Thomas, Höfling, Felix
core   +1 more source

Polyhalogenated Carbazole Impairs Dopaminergic Neurons through Dysregulation of Liquid–Liquid Phase Separation in Caenorhabditis elegans

Advanced Science, EarlyView.
Polyhalogenated carbazoles (PHCZ), persistent environmental contaminants, preferentially accumulate in neuronal tissues. Studies using Caenorhabditis elegans and human neuronal cell lines reveal that PHCZ induce dopaminergic neurodegeneration by promoting liquid–liquid phase separation of α‐synuclein, reducing condensate fluidity, impairing ...
Yuhang Luo, Hongbo Xu, Pei Peng, Xinhe Lu, Qian Zhou, Ziqing Gao, Cheng Tang, Hongyan Yin, Ying Cai, Gaga Mahai, Zhiqiang Zhu, Zuojun Liu, Hanzeng Li, Shunqing Xu   +13 more
wiley   +1 more source

Fluorescence correlation spectroscopy for the characterization of membranes : A short review [PDF]

Songklanakarin Journal of Science and Technology (SJST), 2002
Fluorescence correlation spectroscopy (FCS) is a highly sensitive fluorescence microscopy technique that can be used to probe a wide range of biophysical processes including diffusion, Ligand-receptor binding and molecular aggregation on artificial and ...
Hwang, L.C., Thorsten Wohland
doaj  

Chromatin Dynamics during Lytic Infection with Herpes Simplex Virus 1

Viruses, 2013
Latent HSV-1 genomes are chromatinized with silencing marks. Since 2004, however, there has been an apparent inconsistency in the studies of the chromatinization of the HSV-1 genomes in lytically infected cells.
Luis M. Schang, Kristen L. Conn
doaj   +1 more source