Results 141 to 150 of about 41,336 (313)

Initial Distance‐Dependent Mean Force Drives Synaptic Vesicle Motion Toward Fusion Sites in Stimulated Hippocampal Neurons

Advanced Science, EarlyView.
Synaptic vesicle (SV) motion and exocytosis dynamics in the presynaptic terminals of live neurons are intimately linked to synaptic transmission during neuronal activity. In this work, it is revealed that, during electrical stimulation, SVs located farther from their fusion sites exhibit greater straightness and experience larger mean forces directed ...
Gyunam Park, Ji‐Hyun Kim, Hunki Lee, Chungwon Park, Sidong Chen, Luke Bates, Jaeyoung Sung, Hyokeun Park   +7 more
wiley   +1 more source

Reversing protonation of weakly basic drugs greatly enhances intracellular diffusion and decreases lysosomal sequestration

eLife
For drugs to be active they have to reach their targets. Within cells this requires crossing the cell membrane, and then free diffusion, distribution, and availability.
Debabrata Dey, Shir Marciano, Anna Poryval, Ondřej Groborz, Lucie Wohlrabova, Tomás Slanina, Gideon Schreiber   +6 more
doaj   +1 more source

In Situ Lipoprotein‐seeking Dye for in Vivo Real‐Time Imaging of Lipid Dysregulation Diseases

Advanced Science, EarlyView.
This study develops lipoprotein‐seeking NIR‐II dyes that specifically bind circulating lipoproteins. These dyes form ultra‐stable complexes with endogenous lipoprotein, enabling real‐time, high‐contrast imaging of fatty liver and atherosclerotic plaques. Tunable binding kinetics allow customized imaging windows.
Yijing Du, Zetao Dang, Baofeng Xu, Jiajun Xu, Dan Wang, Yuewei Zhang, Shoujun Zhu   +6 more
wiley   +1 more source

Recent Advances in Fluorescence Recovery after Photobleaching for Decoupling Transport and Kinetics of Biomacromolecules in Cellular Physiology. [PDF]

Polymers (Basel), 2022
Cai N, Lai AC, Liao K, Corridon PR, Graves DJ, Chan V.   +5 more
europepmc   +1 more source

Preservation of Complex Multiphase Architectures in Polymer‐Based Artificial Cells by Photo‐Crosslinking

Advanced Science, EarlyView.
Phase separation organizes cellular components, yet stabilizing such dynamic structures in artificial cells remains challenging. A photo‐crosslinking approach is presented that prolongs the lifetime of different structures in coacervate‐based artificial cells while maintaining dynamic formation.
Madelief A. M. Verwiel, Alexander B. Cook, Yiǧitcan Sümbelli, Nadia A. Erkamp, Jan C. M. van Hest   +4 more
wiley   +1 more source

Theory of fluorescence recovery after photobleaching measurements on cylindrical surfaces

Biophysical Journal, 1992
The theory of fluorescence recovery after photobleaching measurements of isotropic diffusion on a cylindrical surface is developed for Gaussian beam illumination centered perpendicular to an infinitely-long cylinder. A general analytical solution is obtained which is a function of the ratio of the cylindrical radius (r) to the beam exp [-2] radius ...
openaire   +2 more sources

Photophysics of single silicon vacancy centers in diamond: implications for single photon emission

, 2012
Single silicon vacancy (SiV) color centers in diamond have recently shown the ability for high brightness, narrow bandwidth, room temperature single photon emission.
Aharonovich, Aharonovich, Bergman, Bradac, Brouri, Castelletto, Chance, Chapman, Chen, Christoph Becher, Dexter, D’Haenens-Johansson, Elke Neu, Fleury, Grudinkin, Hellen, Iakoubovskii, Kitson, Lounis, Mario Agio, Mora, Neu, Neu, Neu, Neu, Osad’ko, Rogers, Rogobete, Siyushev, Smith, Tisler, Turukhin, Ulbricht, Wang, Wee, Wu   +35 more
core   +1 more source

NPM1 Mediates mRNA Sorting into Extracellular Vesicles via Specific RNA Motif Binding and Phase Separation

Advanced Science, EarlyView.
NPM1 selectively sorts specific mRNAs into extracellular vesicles (EVs) by recognizing RNA motifs and forming phase‐separated condensates. These mRNA cargos—including EGFR—are then loaded via multivesicular bodies. This active sorting pathway, validated in EVs derived from both lung cancer cells and patient serum, reveals a specific mechanism for ...
Kaixiang Zhang, Gaoge Sun, Xiangzheng Liu, Shuai Zuo, Pengyuan Wang, Boxiao Zhan, Zhi John Lu, Hang Yin, Ying Zhang   +8 more
wiley   +1 more source

Osmotic Tension Asymmetry Drives Electrotactic Migration via PDLIM7‐Polarized Microfilament Coordination in Breast Cancer Cells

Advanced Science, EarlyView.
This study establishes an electric field‐induced directional migration model using fluorescence tension probes to visualize microfilament forces and intracellular osmotic pressure dynamics in the electrotactic migration of breast cancer cells. This model delineates how electromechanical interactions among membrane potential, ion channels, OP, traction ...
Ling Zhu, Zihui Zheng, Wang Li, Chenyi Shou, Ying Zhao, Lijun Zhang, Xiaoli Shi, Yunfeng Hu, Huanhuan Zhao, Huiwen Wu, Jun Guo   +10 more
wiley   +1 more source

Correction to: Fluorescence recovery after photobleaching: analyses of cyanobacterial phycobilisomes reveal intrinsic fluorescence recovery. [PDF]

Mar Life Sci Technol, 2021
Zhang N, Li K, Xie BB, Chen XL, Zhou BC, Su HN, Zhang YZ.   +6 more
europepmc   +1 more source