Analysis of Binding Reactions by Fluorescence Recovery after Photobleaching [PDF]
Biophysical Journal, 2004 Fluorescence recovery after photobleaching (FRAP) is now widely used to investigate binding interactions in live cells. Although various idealized solutions have been identified for the reaction-diffusion equations that govern FRAP, there has been no comprehensive analysis or systematic approach to serve as a guide for extracting binding information ...
Sprague, Brian L. +3 more
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Data in Brief, 2016 The presented data provides additional information about the assessment of affinity purified nicotinic acetylcholine receptor (nAChR) rich membrane solubilized with long chain (16 saturated carbons) lysophospholipid with glycerol headgroup (LFG-16).
Luis F. Padilla-Morales +4 more
doaj +1 more source
Modern fluorescent techniques to investigate the mechanisms of lymphocyte activation [PDF]
The Ukrainian Biochemical Journal, 2015 Fluorescent proteins are promising tools for studying intracellular signaling processes in lymphocytes. This brief review summarizes fluorescence-based imaging techniques developed in recent years and discusses new methodological advances, such as ...
G. A. Liubchenko +2 more
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A pharmacological cocktail for arresting actin dynamics in living cells. [PDF]
, 2011 The actin cytoskeleton is regulated by factors that influence polymer assembly, disassembly, and network rearrangement. Drugs that inhibit these events have been used to test the role of actin dynamics in a wide range of cellular processes.
Peng, Grace E +2 more
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Osteoarthritis and Cartilage Open, 2020 Summary: Objective: Articular cartilage is an avascular tissue. Accordingly, diffusivity represents a fundamental transport mechanism for nutrients and other molecular signals regulating its cell metabolism and maintenance of the extracellular matrix ...
Francesco Travascio +2 more
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Effects of Organelle Shape on Fluorescence Recovery after Photobleaching [PDF]
Biophysical Journal, 2005 The determination of diffusion coefficients from fluorescence recovery data is often complicated by geometric constraints imposed by the complex shapes of intracellular compartments. To address this issue, diffusion of proteins in the lumen of the endoplasmic reticulum (ER) is studied using cell biological and computational methods.
Sbalzarini, Ivo F. +3 more
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Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes [PDF]
PeerJ, 2020 Background In this study, we investigated the effect of the mechanical loading history on the expression of receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG) in MLO-Y4 osteocyte-like cells.
Ziyi Wang +7 more
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Unbreakable codes in electrospun fibers : digitally encoded polymers to stop medicine counterfeiting [PDF]
, 2010 Fluorescent polymer solutions can easily be electrospun into micrometer-sized fibers and subsequently encoded with long lasting digital codes by a photobleaching process.
Braeckmans, Kevin +9 more
core +2 more sources
A molecular basis for selective antagonist destabilization of dopamine D3 receptor quaternary organization [PDF]
, 2017 The dopamine D3 receptor (D3R) is a molecular target for both first-generation and several recently-developed antipsychotic agents. Following stable expression of this mEGFP-tagged receptor, Spatial Intensity Distribution Analysis indicated that a ...
Caltabiano, Gianluigi +6 more
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SUMOylation of NaV1.2 channels mediates the early response to acute hypoxia in central neurons. [PDF]
, 2016 The mechanism for the earliest response of central neurons to hypoxia-an increase in voltage-gated sodium current (INa)-has been unknown. Here, we show that hypoxia activates the Small Ubiquitin-like Modifier (SUMO) pathway in rat cerebellar granule ...
Goldstein, Steve An +2 more
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