Results 71 to 80 of about 41,336 (313)

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Live-Cell Imaging of Single Receptor Composition Using Zero-Mode Waveguide Nanostructures [PDF]

, 2012
We exploit the optical and spatial features of subwavelength nanostructures to examine individual receptors on the plasma membrane of living cells. Receptors were sequestered in portions of the membrane projected into zero-mode waveguides.
Dougherty, Dennis A., Korlach, Jonas, Lester, Henry A., Luong, Khai, Richards, Christopher I., Srinivasan, Rahul, Turner, Stephen W.   +6 more
core   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Photoswitching Conduction in Framework Materials

Advanced Functional Materials, EarlyView.
This mini‐review summarizes recent advances in state‐of‐the‐art proton and electron conduction in framework materials that can be remotely and reversibly switched on and off by light. It discusses the various photoswitching conduction mechanisms and the strategies employed to enhance photoswitched conductivity.
Helmy Pacheco Hernandez, Stefan Hecht, Wolfgang Wenzel, Lars Heinke, Mariana Kozlowska   +4 more
wiley   +1 more source

Sizing nanomaterials in bio-fluids by cFRAP enables protein aggregation measurements and diagnosis of bio-barrier permeability

Nature Communications, 2016
Measuring the size distribution of nanomaterials in biological fluids is crucial to understand their properties in vivo. Here, the authors apply fluorescence recovery after photobleaching to measure protein aggregation in serum and to study permeability ...
Ranhua Xiong, Roosmarijn E. Vandenbroucke, Katleen Broos, Toon Brans, Elien Van Wonterghem, Claude Libert, Jo Demeester, Stefaan C. De Smedt, Kevin Braeckmans   +8 more
doaj   +1 more source

FRAP: A Powerful Method to Evaluate Membrane Fluidity in Caenorhabditis elegans

Bio-Protocol, 2018
FRAP (Fluorescence Recovery After Photobleaching) is probably the most direct method to investigate the dynamics of molecules in living cells. Here, we describe FRAP to quantify membrane fluidity in C. elegans.
Ranjan Devkota, Marc Pilon
doaj   +1 more source

Studying Protein Aggregation in the Context of Liquid-liquid Phase Separation Using Fluorescence and Atomic Force Microscopy, Fluorescence and Turbidity Assays, and FRAP

Bio-Protocol, 2020
Liquid-liquid phase separation (LLPS) underlies the physiological assembly of many membrane-less organelles throughout the cell. However, dysregulation of LLPS may mediate the formation of pathological aggregates associated with neurodegenerative ...
William Babinchak, Witold Surewicz
doaj   +1 more source

Upconversion Nanoparticles Embedded Photonic Contact Lens for Transepithelial Corneal Crosslinking Using Hyaluronate – Riboflavin Conjugate

Advanced Functional Materials, EarlyView.
A minimally invasive, transepithelial corneal cross‐linking (TE‐CXL) approach is presented using upconversion nanoparticles (UCNPs)‐loaded contact lenses (UCLs), after topical delivery of hyaluronate–riboflavin conjugates. The NIR‐to‐UV/blue light conversion by UCNPs in a UCL can activate riboflavin for TE‐CXL, resulting in the biomechanical strength ...
Gibum Lee, Inhoo Choi, Anara Molkenova, Yengwoo Son, Seungmin Yang, Mina Kwon, Tae‐Im Kim, Ki Su Kim, Sei Kwang Hahn   +8 more
wiley   +1 more source

Dynamic Mode Decomposition of Fluorescence Loss in Photobleaching Microscopy Data for Model-Free Analysis of Protein Transport and Aggregation in Living Cells

Sensors, 2022
The phase separation and aggregation of proteins are hallmarks of many neurodegenerative diseases. These processes can be studied in living cells using fluorescent protein constructs and quantitative live-cell imaging techniques, such as fluorescence ...
Daniel Wüstner
doaj   +1 more source

Bio‐Orthogonally Crosslinked Supramolecular Polymer Bottlebrush Hydrogels for Long‐Term 3D Cell Culture

Advanced Functional Materials, EarlyView.
Fibrous benzenetrispeptide (BTP) hydrogels, fabricated via strain‐promoted azide‐alkyne cycloaddition (SPAAC) crosslinking, form robust, bioinert networks. These hydrogels can support 3D cell culture, where cell viability and colony growth depend on the fiber content.
Ceren C. Pihlamagi, David Pretzel, Peer Nölte, Kourosh Rezaei, Ulrich S. Schubert, Johannes C. Brendel   +5 more
wiley   +1 more source