Results 81 to 90 of about 40,790 (312)
QUANTITATIVE CONFOCAL LASER SCANNING MICROSCOPY
Image Analysis and Stereology, 2011 This paper discusses recent advances in confocal laser scanning microscopy (CLSM) for imaging of 3D structure as well as quantitative characterization of biomolecular interactions and diffusion behaviour by means of one- and two-photon excitation.
Merete Krog Raarup +1 more
doaj +1 more source
Protein Mobility in the Cytoplasm of Escherichia coli [PDF]
, 1999 The rate of protein diffusion in bacterial cytoplasm may constrain a variety of cellular functions and limit the rates of many biochemical reactions in vivo. In this paper, we report noninvasive measurements of the apparent diffusion coefficient of green
Elowitz, Michael B. +4 more
core +2 more sources
Live-Cell Imaging of Single Receptor Composition Using Zero-Mode Waveguide Nanostructures [PDF]
, 2012 We exploit the optical and spatial features of subwavelength nanostructures to examine individual receptors on the plasma membrane of living cells. Receptors were sequestered in portions of the membrane projected into zero-mode waveguides.
Dougherty, Dennis A. +6 more
core +1 more source
Geometrically Tunable Scaffold‐Free Muscle Bioconstructs for Treating Volumetric Muscle Loss
Advanced Healthcare Materials, EarlyView.Volumetric muscle loss is associated with traumatic muscle resulting in permanent functional impairment. Mold‐based, scaffold‐free, high‐density muscle tissue bioconstructs are developed in customizable geometric shapes and sizes. The transplanted rectangular solid‐shaped muscle bioconstructs improved muscle force recovery and tissue regeneration in ...
Bugra Ayan +8 more
wiley +1 more source
STAR Protocols, 2021 Summary: Fluorescence recovery after photobleaching (FRAP) has been widely used to monitor membrane properties by measuring the lateral diffusion of fluorescent particles.
Shefin S. George +2 more
doaj +1 more source
Advanced Healthcare Materials, EarlyView.Design rules are presented to control intestinal organoid polarity in fully synthetic hydrogels. The laminin‐derived IKVAV sequence is crucial to obtain correct intestinal organoid polarity. Increasing hydrogel dynamics further supports the growth of correctly polarized intestinal organoids, while a bulk level of stiffness (G’ ≈ 0.7 kPa) is crucial to ...
Laura Rijns +10 more
wiley +1 more source
eLife, 2018 Dietary fatty acids are the main building blocks for cell membranes in animals, and mechanisms must therefore exist that compensate for dietary variations. We isolated C.
Mario Ruiz +8 more
doaj +1 more source
Time-lapse imaging of Drosophila testis for monitoring actin dynamics and sperm release
STAR Protocols, 2022 Summary: Here we describe a simple step-by-step protocol for collecting high-resolution, time-lapse images of intact Drosophila testis ex vivo for a limited period using a confocal microscope, with minimum photo-toxic damage, to monitor spermatid ...
Tushna Kapoor +2 more
doaj +1 more source
Structural changes in cartilage and collagen studied by high temperature Raman spectroscopy [PDF]
, 2017 Understanding the high temperature behavior of collagen and collagenous tissue is important for surgical procedures and biomaterials processing for the food, pharmaceutical, and cosmetics industries. One primary event for proteins is thermal denaturation
Dudhia, J +3 more
core +3 more sources
Transforming Cancer Diagnosis and Therapy Through Fluorescent Hydrogels: A Review
Advanced Healthcare Materials, EarlyView.This article reviews fluorescent hydrogel systems for cancer detection, monitoring, and therapy. Mimicking the tumor microenvironment, these hydrogels enable real‐time imaging and targeted drug delivery. The review discusses design strategies, labeling techniques, and applications, highlighting challenges and future opportunities in cancer diagnostics ...
Elahe Masaeli +2 more
wiley +1 more source