Results 81 to 90 of about 41,336 (313)

Fluorescence Recovery after Photobleaching (FRAP) Assay to Measure the Dynamics of Fluorescence Tagged Proteins in Endoplasmic Reticulum Membranes of Plant Cells

open access: yesBio-Protocol, 2014
In this protocol, we used fluorescence recovery after photobleaching (FRAP) to measure the influence that some mutations and drug treatment have on mobility of a green fluorescent protein (GFP)-fused viral transmembrane protein into endoplasmic reticulum
José Navarro   +2 more
doaj   +1 more source

Effects of a synthetic bioactive peptide on neurite growth and nerve growth factor release in chondroitin sulfate hydrogels. [PDF]

open access: yes, 2011
Previous work has revealed robust dorsal root ganglia neurite growth in hydrogels of chondroitin sulfate. In the current work, it was determined whether addition of a synthetic bioactive peptide could augment neurite growth in these matrices via enhanced
Beier, Brooke L   +3 more
core   +1 more source

Color Routing and Beam Steering of Single‐Molecule Emission with a Spherical Silicon Nanoantenna

open access: yesAdvanced Functional Materials, EarlyView.
We experimentally demonstrate broadband directional emission from single molecules using a single spherical silicon nanoparticle assembled via DNA origami. By varying nanoparticle (NP) size and emitter position, we achieve unidirectional emission, beam steering, and color routing at the nanoscale, revealing modal interference as the underlying ...
María Sanz‐Paz   +9 more
wiley   +1 more source

Membrane Fusion‐Inspired Nanomaterials: Emerging Strategies for Infectious Disease and Cancer Diagnostics

open access: yesAdvanced Healthcare Materials, EarlyView.
Membrane fusion‐inspired nanomaterials offer transformative potential in diagnostics by mimicking natural fusion processes to achieve highly sensitive and specific detection of disease biomarkers. This review highlights recent advancements in nanomaterial functionalization strategies, signal amplification systems, and stimuli‐responsive fusion designs,
Sojeong Lee   +9 more
wiley   +1 more source

QUANTITATIVE CONFOCAL LASER SCANNING MICROSCOPY

open access: yesImage Analysis and Stereology, 2011
This paper discusses recent advances in confocal laser scanning microscopy (CLSM) for imaging of 3D structure as well as quantitative characterization of biomolecular interactions and diffusion behaviour by means of one- and two-photon excitation.
Merete Krog Raarup   +1 more
doaj   +1 more source

Protein Mobility in the Cytoplasm of Escherichia coli [PDF]

open access: yes, 1999
The rate of protein diffusion in bacterial cytoplasm may constrain a variety of cellular functions and limit the rates of many biochemical reactions in vivo. In this paper, we report noninvasive measurements of the apparent diffusion coefficient of green
Elowitz, Michael B.   +4 more
core   +2 more sources

Geometrically Tunable Scaffold‐Free Muscle Bioconstructs for Treating Volumetric Muscle Loss

open access: yesAdvanced Healthcare Materials, EarlyView.
Volumetric muscle loss is associated with traumatic muscle resulting in permanent functional impairment. Mold‐based, scaffold‐free, high‐density muscle tissue bioconstructs are developed in customizable geometric shapes and sizes. The transplanted rectangular solid‐shaped muscle bioconstructs improved muscle force recovery and tissue regeneration in ...
Bugra Ayan   +8 more
wiley   +1 more source

Controlling Intestinal Organoid Polarity using Synthetic Dynamic Hydrogels Decorated with Laminin‐Derived IKVAV Peptides

open access: yesAdvanced Healthcare Materials, EarlyView.
Design rules are presented to control intestinal organoid polarity in fully synthetic hydrogels. The laminin‐derived IKVAV sequence is crucial to obtain correct intestinal organoid polarity. Increasing hydrogel dynamics further supports the growth of correctly polarized intestinal organoids, while a bulk level of stiffness (G’ ≈ 0.7 kPa) is crucial to ...
Laura Rijns   +10 more
wiley   +1 more source

Membrane fluidity is regulated by the C. elegans transmembrane protein FLD-1 and its human homologs TLCD1/2

open access: yeseLife, 2018
Dietary fatty acids are the main building blocks for cell membranes in animals, and mechanisms must therefore exist that compensate for dietary variations. We isolated C.
Mario Ruiz   +8 more
doaj   +1 more source

A two-photon FRAP protocol to measure the stereociliary membrane diffusivity in rat cochlear hair cells

open access: yesSTAR Protocols, 2021
Summary: Fluorescence recovery after photobleaching (FRAP) has been widely used to monitor membrane properties by measuring the lateral diffusion of fluorescent particles.
Shefin S. George   +2 more
doaj   +1 more source

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