Results 11 to 20 of about 1,322,177 (348)

Fluorescence polarization of green fluorescence protein [PDF]

Proceedings of the National Academy of Sciences, 2002
We report here the striking anisotropy of fluorescence exhibited by crystals of native green fluorescence protein (GFP). The crystals were generated by water dialysis of highly purified GFP obtained from the jellyfish Aequorea .
Shinya, Inoué, Osamu, Shimomura, Makoto, Goda, Mykhailo, Shribak, P T, Tran   +4 more
openaire   +3 more sources

Snake Toxins Labeled by Green Fluorescent Protein or Its Synthetic Chromophore are New Probes for Nicotinic acetylcholine Receptors

Frontiers in Molecular Biosciences, 2021
Fluorescence can be exploited to monitor intermolecular interactions in real time and at a resolution up to a single molecule. It is a method of choice to study ligand-receptor interactions.
Igor E. Kasheverov, Alexey I. Kuzmenkov, Denis S. Kudryavtsev, Ivan S. Chudetskiy, Irina V. Shelukhina, Evgeny P. Barykin, Igor A. Ivanov, Andrei E. Siniavin, Rustam H. Ziganshin, Mikhail S. Baranov, Victor I. Tsetlin, Alexander A. Vassilevski, Alexander A. Vassilevski, Yuri N. Utkin   +13 more
doaj   +1 more source

Teaching an old ‘doc’ new tricks for algal biotechnology: Strategic filter use enables multi-scale fluorescent protein signal detection

Frontiers in Bioengineering and Biotechnology, 2022
Fluorescent proteins (FPs) are powerful reporters with a broad range of applications in gene expression and subcellular localization. High-throughput screening is often required to identify individual transformed cell lines in organisms that favor non ...
Sergio Gutiérrez, Gordon B. Wellman, Kyle J. Lauersen   +2 more
doaj   +1 more source

Erasable labeling of neuronal activity using a reversible calcium marker

eLife, 2020
Understanding how the brain encodes and processes information requires the recording of neural activity that underlies different behaviors. Recent efforts in fluorescent protein engineering have succeeded in developing powerful tools for visualizing ...
Fern Sha, Ahmed S Abdelfattah, Ronak Patel, Eric R Schreiter   +3 more
doaj   +1 more source

Using a Riboswitch Sensor to Detect Co2+/Ni2+ Transport in E. coli

Frontiers in Chemistry, 2021
Intracellular concentrations of essential mental ions must be tightly maintained to avoid metal deprivation and toxicity. However, their levels in cells are still difficult to monitor. In this report, the combination of a Co2+Ni2+-specific riboswitch and
Xiaoying Wang, Wei Wei, Wei Wei, Jing Zhao   +3 more
doaj   +1 more source

Superinfection Exclusion of Alphaherpesviruses Interferes with Virion Trafficking

Microbiology Spectrum, 2022
Superinfection exclusion (SIE) is a phenomenon in which a primary viral infection interferes with secondary viral infections within that same cell. Although SIE has been observed across many viruses, it has remained relatively understudied.
James P. Cwick, Jonathan E. Owen, Irina Kochetkova, Kyle S. Hain, Nick Van Horssen, Matthew P. Taylor   +5 more
doaj   +1 more source

Developing a Universal and Efficient Method for the Rapid Selection of Stable Fluorescent Protein-Tagged Pathogenic Vibrio Species

Journal of Marine Science and Engineering, 2020
World-wide increases in Vibrio-associated diseases have been reported in aquaculture and humans in co-occurrence with increased sea surface temperatures. Twelve species of Vibrio are known to cause disease in humans, but three species dominate the number
Candice A. Thorstenson, Matthias S. Ullrich   +1 more
doaj   +1 more source

Using Scuba for In Situ Determination of Chlorophyll Distributions in Corals by Underwater Near Infrared Fluorescence Imaging

Journal of Marine Science and Engineering, 2020
Studies reporting quantitation and imaging of chlorophyll in corals using visible fluorescent emission in the red near 680 nm can suffer from competing emission from other red-emitting pigments.
Thomas Oh, Jittiwat Sermsripong, Barry W. Hicks   +2 more
doaj   +1 more source

FLUORESCENT PROTEINS USING FOR LYMPHOCYTE ACTIVATION ASSAYING [PDF]

Biotechnologia Acta, 2013
Activation of immune cells is a key process in development of the specific immunity. The techniques for monitoring of the movement and the activity of signalling messengers and receptor proteins of lymphocytes in living cells and tissues are the ...
G. A. Lubchenko, R. G. Morev, L. S. Holodnaya, L. I. Ostapchenko   +3 more
doaj   +1 more source

CemOrange2 fusions facilitate multifluorophore subcellular imaging in C. elegans [PDF]

, 2019
Due to its ease of genetic manipulation and transparency, Caenorhabditis elegans (C. elegans) has become a preferred model system to study gene function by microscopy.
Buland, Justin R, Chou, Wendy Y. Y., Cole, F. Sessions, Dawson, Zachary, Homayouni, Airelle, Huang, Huiyan, Jia, Hanna, Kim, Hyori, Luke, Cliff J, Moreno, Marco, Pak, Stephen C, Silverman, Gary A, Thomas, Brian J, Wambach, Jennifer A, Wright, Ira E   +14 more
core   +3 more sources