Results 41 to 50 of about 1,328,987 (298)
Homotransfer FRET Reporters for Live Cell Imaging
Biosensors, 2018 Förster resonance energy transfer (FRET) between fluorophores of the same species was recognized in the early to mid-1900s, well before modern heterotransfer applications.
Nicole E. Snell +6 more
doaj +1 more source
Frontiers in Chemistry, 2021 Single Molecule Localization Microscopy (SMLM) is an imaging method that allows for the visualization of structures smaller than the diffraction limit of light (~200 nm).
Pooja Laxman +3 more
doaj +1 more source
Fluorescent Probes for STED Optical Nanoscopy
Nanomaterials, 2021 Progress in developing fluorescent probes, such as fluorescent proteins, organic dyes, and fluorescent nanoparticles, is inseparable from the advancement in optical fluorescence microscopy.
Sejoo Jeong +2 more
doaj +1 more source
A bidirectional fluorescent two-hybrid system for monitoring protein–protein interactions
, 2014 Two-hybrid systems have been the cornerstone of research into protein–protein interactions, but these systems typically rely on life/death reporters that put additional selective pressure on the host organism, and potentially lead to false positives ...
Nordgren, Ida Karin, Tavassoli, Ali
core +1 more source
Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective. [PDF]
, 2017 Surface enhanced Raman scattering (SERS) nanoparticles are an attractive alternative to fluorescent probes for biological labeling because of their photostability and multiplexing capabilities.
Candas, Demet +7 more
core +1 more source
CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana [PDF]
, 2016 Background: The in vivo determination of the cell-specific chromosome number provides a valuable tool in several aspects of plant research. However, current techniques to determine the endosystemic ploidy level do not allow non-destructive, cell-specific
Angenon, Geert +4 more
core +5 more sources
Biosensors, 2019 In previous studies, we encountered substantial problems using the CFP_YFP Förster resonance energy transfer (FRET) pair to analyze protein proximity in the endoplasmic reticulum of live cells.
Kira Erismann-Ebner +2 more
doaj +1 more source
Direction dependent mechanical unfolding and Green Fluorescent Protein as a force sensor [PDF]
, 2011 An Ising--like model of proteins is used to investigate the mechanical unfolding of the Green Fluorescent Protein along different directions. When the protein is pulled from its ends, we recover the major and minor unfolding pathways observed in ...
Caraglio, M. +2 more
core +2 more sources
Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source
FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source