Results 51 to 60 of about 1,328,987 (298)

Three-Color Imaging Using Fluorescent Proteins in Living Zebrafish Embryos

BioTechniques, 2001
The zebrafish embryo is especially valuable for cell biological studies because of its optical clarity. In this system, use of an in vivo fluorescent reporter has been limited to green fluorescent protein (GFP).
Kenneth R. Finley, Ann E. Davidson, Stephen C. Ekker   +2 more
doaj   +1 more source

An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots

FEBS Letters, EarlyView.
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino, Meijie Li, Cécile Fizames, Mattia Adamo, Laurence Lejay, Christian Dubos, Matthieu Platre, Antoine Martin   +7 more
wiley   +1 more source

Genetically encoded fluorescent redox probes. [PDF]

, 2013
Redox processes are involved in almost every cell of the body as a consequence of aerobic life. In the past decades, redox biology has been increasingly recognized as one of the key themes in cell signaling.
Ai, Hui-Wang, Ren, Wei
core   +3 more sources

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

Knock-In Mouse Models to Investigate the Functions of Opioid Receptors in vivo

Frontiers in Cellular Neuroscience, 2022
Due to their low expression levels, complex multi-pass transmembrane structure, and the current lack of highly specific antibodies, the assessment of endogenous G protein-coupled receptors (GPCRs) remains challenging. While most of the research regarding
Jade Degrandmaison, Jade Degrandmaison, Jade Degrandmaison, Samuel Rochon-Haché, Samuel Rochon-Haché, Samuel Rochon-Haché, Jean-Luc Parent, Louis Gendron, Louis Gendron   +8 more
doaj   +1 more source

Fluorescent Plasmodium berghei sporozoites and pre-erythrocytic stages: a new tool to study mosquito and mammalian host interactions with malaria parasites.

, 2001
To track malaria parasites for biological studies within the mosquito and mammalian hosts, we constructed a stably transformed clonal line of Plasmodium berghei, PbFluspo, in which sporogonic and pre-erythrocytic liver-stage parasites are autonomously ...
Hafalla, JC, Mota, MM, Ménard, R, Natarajan, R, Thathy, V, Vernick, KD   +5 more
core   +1 more source

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]

, 2012
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Abbe, Abbe, Ai, Airy, Alivisatos, Alivisatos, Ando, Aoki, Auksorius, Auzel, Auzel, Axelrod, Axelrod, Bacskai, Bader, Bader, Balse, Barroso, Bastiaens, Bates, Beaudouin, Beaurepaire, Becker, Benninger, Berezin, Berney, Betzig, Bianchini, Biju, Binnemans, Borst, Brakemann, Bretschneider, Bunt, Campagnola, Campagnola, Campbell, Cantor, Carrero, Chalfie, Chalfie, Chatterjee, Chen, Chen, Clapp, Clapp, Clapp, Cole, Coons, Dahan, Dedecker, Del Pozo, Deng, Denk, Diaspro, Diaspro, Dickson, Digman, Drobizhev, Drummen, Dundr, Dundr, Dunn, Dunn, Dunn, Durr, Dyba, Ehrlicher, Einstein, Elangovan, Elder, Ellenberg, Elsner, Feige, Fereidouni, Festenstein, Frommer, Förster, Förster, Gamelin, Garcia-Mira, Garcia-Parajo, Gerlich, Giese, Giordano, Goodson, Gopich, Gordon, Gregor P. C. Drummen, Grotjohann, Gu, Gunnlaugsson, Gurskaya, Gustafsson, Göppert-Mayer, Ha, Haugland, Heimstädt, Hellen C. Ishikawa-Ankerhold, Hemmila, Hiller, Hitakomate, Hofkens, Hofmann, Houtsmuller, Houtsmuller, Howell, Hu, Hu, Huang, Ishihama, Jacobson, Jares-Erijman, Jiang, Johannes, Johnson, Jonkman, Jose, Jovin, Jovin, Kaiser, Kambara, Kapustina, Karasawa, Kardash, Kasha, Kawashima, Kenworthy, Kerppola, Kerppola, Kerppola, Kerppola, Kimura, Kinoshita, Klonis, Kner, Knoblauch, Konig, Koppel, Koster, Kota, Koushik, Kremers, Kremers, Kuningas, Kuningas, Kuningas, Laforge, Lakowicz, Lakowicz, Larson, Lee, Lichtman, Liebman, Lippincott-Schwartz, Liu, Llères, Loura, Lukyanov, Lundin, Mansfield, Mathew-Fenn, Mattheyses, Matz, Medintz, Mills, Mitchell, Miyawaki, Miyawaki, Moerner, Moneron, Monetta, Mueller, Mueller, Nagai, Nehls, Ormö, Paddock, Parsons, Patterson, Patterson, Patterson, Pawley, Pawley, Persechini, Peters, Phair, Phair, Phillips, Piston, Piston, Planck, Pockwinse, Politz, Poo, Puliti, Rajaram, Rantanen, Reinhard, Reits, Reymann, Richard Ankerhold, Richards, Riven, Rizzo, Rizzo, Robinson, Roduner, Rotblat, Roy, Rust, Sakon, Sbalzarini, Schawlow, Schönle, Seabrooke, Seah, Selvin, Shaner, Shav-Tal, Shaw, Shchyolkina, Shimomura, Shimomura, Shroff, Shu, Shyu, Smith, So, Solon, Soukka, Soumpasis, Sprague, Sprague, Stasevich, Stelzer, Stepanenko, Stokes, Stryer, Stryer, Subach, Sun, Suyver, Swaminathan, Swift, Sönnichsen, Tagawa, Taylor, Thaler, Tian, Tian, Tinnefeld, Tisler, Tocanne, Tomosugi, Tripathi, Tsien, Tsien, Uphoff, Valentin, van Driel, Van Munster, Van Munster, van Munster, van Rheenen, van Royen, van Royen, van Royen, Varghese, Venetta, Vogelsang, von Wichert, Vosch, Wallrabe, Wang, Wang, Wang, Wang, Wang, Wang, Welch, White, Williams, Willig, Wouters, Wouters, Wouters, Wouters, Wright, Xia, Yang, Youvan, Zhang, Zhang, Zimmer, Zipfel   +295 more
core   +2 more sources

The Caenorhabditis elegans DPF‐3 and human DPP4 have tripeptidyl peptidase activity

FEBS Letters, EarlyView.
The dipeptidyl peptidase IV (DPPIV) family comprises serine proteases classically defined by their ability to remove dipeptides from the N‐termini of substrates, a feature that gave the family its name. Here, we report the discovery of a previously unrecognized tripeptidyl peptidase activity in DPPIV family members from two different species.
Aditya Trivedi, Rajani Kanth Gudipati
wiley   +1 more source

Genetically encoded fluorescent biosensors for GPCR research

Frontiers in Cell and Developmental Biology, 2022
G protein-coupled receptors (GPCRs) regulate a wide range of physiological and pathophysiological cellular processes, thus it is important to understand how GPCRs are activated and function in various cellular contexts.
Hyunbin Kim, Hyunbin Kim, In-Yeop Baek, In-Yeop Baek, Jihye Seong, Jihye Seong, Jihye Seong   +6 more
doaj   +1 more source

Fluorescent protein tagging confirms the presence of ribosomal proteins atDrosophilapolytene chromosomes [PDF]

, 2013
\ud \ud Most ribosomal proteins (RPs) are stoichiometrically incorporated into ribosomal subunits and play essential roles in ribosome biogenesis and function.
Bischof, Bohnsack, Brogna, Coleno-Costes, Dai, Dai, De, De, Freeman, Gavin, Gerlitz, Hurt, Inada, Johansen, Jurica, Klinge, Kondrashov, Lam, Lee, Malygin, Marygold, Ni, Ramanathan, Shi, Shopland, Steitz, Warner, Zhimulev   +27 more
core   +3 more sources