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Neutral Red as a Lipid Fluorochrome
Stain Technology, 1970Aqueous 0.1% neutral red adjusted to pH 6.5, or mixed 1:1 with seawater, produced conspicuous fluorescence in plant and animal fats while acting as a conventional stain for acidic groups including volutin. The water-soluble, nonfluorescent dye predominating in acid solutions became deprotonized, lipophilic and fluorescent under neutral and alkaline ...
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A Dual Fluorochrome Probe for Imaging Proteases
Bioconjugate Chemistry, 2004Near-infrared fluorescence (NIRF) optical probes have been able to provide a noninvasive assessment of enzyme activity for a number of different enzymes and types of pathology. Here we describe a dual fluorochrome enzyme-activatable probe featuring one NIRF fluorochrome that is activated by protease activity and a second fluorochrome that is protease ...
Ralph Weissleder +2 more
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Fluorochromes: Properties and Characteristics
2009Immunofluorescence microscopy provides a sensitive means by which antigens can be localized within tissues or individual cells. For the most effective use of this technique the researcher can draw upon basic information on factors that affect the brightness of the fluorescence image, and how well that image can be distinguished from background ...
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Selective `in synthesis' labelling of peptides by fluorochromes
Biochimica et Biophysica Acta (BBA) - General Subjects, 1997A new method is described for producing fluorescently-tagged peptides containing specific internal derivatives of lysyl residues. The technique employs the base-labile Boc-Lys(Fmoc)-COOH derivative with base-catalyzed removal of the Fmoc protecting group during peptide synthesis and subsequent fluorescent derivatization of the deprotected epsilon-amino
Alberto Chersi +2 more
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Visualization of chloroplast DNA with two fluorochromes
Experimental Cell Research, 1978Abstract A simple and rapid procedure is described in which the fluorochromes 33258 Hoechst and DAPI were used to stain algal cells. Three types of extranuclear fluorescent particles could be distinguished. Type 1 particles, which fluoresced with an orange color, were polyphosphate granules.
Annette W. Coleman, Annette W. Coleman
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Fluorochromes and Fluorescence
2007To facilitate flow cytometry, there is a need for cells to be labeled with fluorochromes. However, because of the spectral properties of fluorochromes, the fluorescence associated with one fluorochrome may be detected by more than one detector, producing spectral overlap which requires compensation.
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Polyene dyes and fluorochromes
2020Polyene dyes and fluorochromes contain linked methine groups forming a chain of trans-oriented conjugated carbon-carbon double bonds, as shown below in structure a. In the naturally occurring carotenoids these are lipophilic alicyclic or aliphatic groups (as in lycopene) or hydrophilic groups such as hydroxy or carboxyl (as in crocetin).
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Fluorochrome Choices for Flow Cytometry
2018Multicolor flow cytometry requires thorough knowledge not only of your proteins of interest, but also of the corresponding fluorochromes that will be used in your panel. Not all fluorochromes are bright, some are prone to photobleaching, and others are sensitive to fixation and permeabilization.
Christopher Hammerbeck, Christine Goetz
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Bone, 2005
Bone formation and remodeling in vivo can be assessed by polychrome labeling using calcium-binding fluorescent dyes. The number of fluorochromes, however, limits this technique due to the fact that with increasing number, fluorescent spectra inevitably overlap, which makes discrimination more difficult.
Matthias Schieker +7 more
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Bone formation and remodeling in vivo can be assessed by polychrome labeling using calcium-binding fluorescent dyes. The number of fluorochromes, however, limits this technique due to the fact that with increasing number, fluorescent spectra inevitably overlap, which makes discrimination more difficult.
Matthias Schieker +7 more
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Thermal decomposition of ammonium fluorochromates
Journal of Fluorine Chemistry, 1991Abstract Quasi static thermoanalytical methods were used to investigate the thermal decomposition of (NH 4 ) 2 [CrF 5 (H 2 O)], (NH 4 ) 3 CrF 6 and (NH 4 ) 2 [Cr(H 2 O) 6 ]F 5 . The thermal decomposition is influenced by the partial pressure of the reaction gases and it succeeds to separate several steps of the decomposition by using quasi-isobar ...
U. Bentrup, D.-H. Menz
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