A pipeline for targeted metagenomics of environmental bacteria. [PDF]
BackgroundMetagenomics and single cell genomics provide a window into the genetic repertoire of yet uncultivated microorganisms, but both methods are usually taxonomically untargeted.
Bowers, Robert M+7 more
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Comparison of the TaqMan and LightCycler systems in pharmacogenetic testing: evaluation of the CYP2C9*2/*3 polymorphisms. [PDF]
Background: Pharmacogenetic testing for drugmetabolizing enzymes is not yet widely used in clinical practice. Methods: In an attempt to facilitate the application of this procedure, we have compared two real-time PCRbased methods, the TaqMan_ and the ...
C. ESPOSITO+5 more
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Diagnosis of anaplastic large-cell lymphoma in a dog using CD30 immunohistochemistry [PDF]
Anaplastic large-cell lymphoma or null-cell lymphoma is a clinical entity reported in people, classified according to the unique appearance of large pleomorphic cells that express CD30.
Garden, O A+7 more
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Contributions of in situ hybridization of sRNA to the study on spatio-temporal gene expression in Hevea brasiliensis [PDF]
Since the dawn of the genomics era, much research has focused on functional studies of genes of interest. In situ hybridization is a method that can be used to precisely localize the expression of a gene in tissues and cells.
Alemanno, Laurence+6 more
core
Cultural methods of detection for microorganisms: recent advances and successes [PDF]
Most microbiological methods require culture to allow organisms to recover or to selectively increase, and target organisms are identified by growth on specific agar media.
Jian, X., Watkins , J.
core
Detection of t(7;12)(q36;p13) in paediatric leukaemia using dual colour fluorescence in situ hybridisation [PDF]
The identification of chromosomal rearrangements is of utmost importance for the diagnosis and classification of specific leukaemia subtypes and therefore has an impact on therapy choices in individual cases.
Federico, C+4 more
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\u3csup\u3e99m\u3c/sup\u3eTc-Labeled C2A Domain of Synaptotagmin I as a Target-Specific Molecular Probe for Noninvasive Imaging of Acute Myocardial Infarction [PDF]
: The exposure of phosphatidylserine (PtdS) is a common molecular marker for both apoptosis and necrosis and enables the simultaneous detection of these distinct modes of cell death. Our aim was to develop a radiotracer based on the PtdS-binding activity
Fang, Wei+7 more
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Understanding Health and Disease with Multidimensional Single-Cell Methods
Current efforts in the biomedical sciences and related interdisciplinary fields are focused on gaining a molecular understanding of health and disease, which is a problem of daunting complexity that spans many orders of magnitude in characteristic length
Banavar, Jayanth R.+2 more
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Flourescent, online monitoring of PLGA degradation for regenerative medicine applications [PDF]
Degradable polymers such as poly(lactic-co-glycolic acid) (PLGA) are frequently chosen for tissue engineering, due to their ease of production, controllable degradation rates and Food and Drug Administration (FDA) approval.
Bardsley, K+3 more
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Comparative cytogenetic study of three Macrolophus species (Heteroptera, Miridae.) [PDF]
Macrolophus pygmaeus (Rambur, 1839) (Insecta, Heteroptera, Miridae) is a predator of key vegetable crop pests applied as a biocontrol agent in the Mediterranean region. M. pygmaeus and M. melanotoma (A.
Agustí Abella, Núria+5 more
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