Results 91 to 100 of about 259,276 (311)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Spatial variability of nitrous oxide in the Minho and Lima estuaries (Portugal)

Scientia Marina, 2017
Nitrous oxide (N2O) is a potent long-lived greenhouse gas and estuaries represent potentially important sources of this biogas to the atmosphere. In this work, we analyse the first N2O data obtained in the Minho and Lima estuaries, and the processes and ...
Célia Gonçalves, Maria José Brogueira
doaj   +1 more source

Partitioning ecosystem scale carbon fluxes into photosynthetic and respiratory components with emphasis on dynamics in δ13CO2

, 2008
Unger S. Partitioning ecosystem scale carbon fluxes into photosynthetic and respiratory components with emphasis on dynamics in δ13CO2.
Unger, Stephan ; https://orcid.org/
core  

Meteorology and surface energy fluxes in the 2005–2007 ablation seasons at the Miage debris-covered glacier, Mont Blanc Massif, Italian Alps

, 2010
During the 2005–2007 June–September ablation seasons, meteorological conditions were recorded on the lower and upper parts of the debris‐covered ablation zone of Miage Glacier, Italy. In 2005, debris temperature and subdebris ice melt were also monitored
Mihalcea, Claudia, Diolaiuti, Guglielmina, G.A. Diolaiuti, Brock, Ben W., Kirkbride, Martin P., M. Kirkbride, C.E. Mihalcea, Cutler, Mark E. J.; id_orcid, C. Smiraglia, M. E. J. Cutler, Cutler, Mark, Brock, Benjamin, Kirkbride, Martin, Smiraglia, Claudio, B. W. Brock   +14 more
core   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy

FEBS Open Bio, EarlyView.
This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan, Ikram A. Burney, Shika Hanif Malgundkar, Siva Ramamoorthy, Ressin Varghese, Mohamed A. Al‐Kindi, Hamad Al Riyami, Shadia Al Bahlani, Sergey Dobretsov, Syed Imran Hassan, Benjamin K. Tsang, Yahya Tamimi   +11 more
wiley   +1 more source

Crescimento vegetativo de plantas cítricas no norte e noroeste do Paraná Vegetative growth of citrus trees in north an northwest of Parana

Revista Brasileira de Fruticultura, 2005
Este trabalho avaliou, em Paranavaí e Londrina-PR, os fluxos de crescimento vegetativo dos ramos de laranjeira 'Folha Murcha' enxertada sobre limoeiro 'Cravo' (Citrus limonia Osb.), limoeiro 'Volcameriano' (Citrus volkameriana Ten. e Pasq.), tangerineira
Neusa Maria Colauto Stenzel, Carmen Silvia Vieira Janeiro Neves, Celso Jamil Marur, José Carlos Gomes   +3 more
doaj   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source

Global mapping of flux and microbial sources for oceanic N2O

Nature Communications
The ocean is the largest source of N2O emissions from global aquatic ecosystems. However, the N2O production–consumption mechanism and microbial spatial distribution are still unclear.
Shuo Wang, Jilin Huang, Zhen Wu, Shengjie Li, Xianfang Zhu, Yong Liu, Guodong Ji   +6 more
doaj   +1 more source

Functional and Structural Evidence of Neurofluid Circuit Aberrations in Huntington Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Disrupted neurofluid regulation may contribute to neurodegeneration in Huntington disease (HD). Because neurofluid pathways influence waste clearance, inflammation, and the distribution of central nervous system (CNS)–delivered therapeutics, understanding their dysfunction is increasingly important as targeted treatments emerge.
Kilian Hett, Abigail Dubois, Melanie Leguizamon, Alexander Song, Paula Trujillo, Colin D. McKnight, Ciaran M. Considine, Manus J. Donahue, Daniel O. Claassen   +8 more
wiley   +1 more source