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SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
FEBS Open Bio, EarlyView.This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.What factors make for an effective digital learning tool in Higher Education? This systematic review identifies elements of a digital tool that published examples reveal to be features of an engaging and impactful digital tool. A systematic literature search yielded 25 research papers for analysis.
Akmal Arzeman +4 more
wiley +1 more source
Formative assessment in project-oriented learning to improve academic performance [PDF]
, 2010 Aguado, RM +3 more
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2014
Formative assessment has received international attention as an instructional approach that has great potential to improve teaching and learning. The concept has roots in educational evaluation practices and has evolved over time, from a focus on formative evaluation to formative assessment or assessment for learning.
+7 more sources
Formative assessment has received international attention as an instructional approach that has great potential to improve teaching and learning. The concept has roots in educational evaluation practices and has evolved over time, from a focus on formative evaluation to formative assessment or assessment for learning.
+7 more sources