Results 181 to 190 of about 59,619 (217)
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Normal tRNAs promote ribosomal frameshifting
Cell, 1979The addition of Ser AGC AGU tRNA to an E. coli cell-free protein synthesizing system which contains the endogenous tRNA levels results in up to 100% of the ribosomes translating the MS2 coat gene shifting into the -1 reading frame. An analogous phenomenon is seen at a much lower level without the tRNA addition, where a shift into the +1 frame can also ...
J F, Atkins +3 more
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Dynamics of +1 ribosomal frameshifting
Mathematical Biosciences, 2014It has been well characterized that the amino acid starvation can induce +1 frameshifting. However, how the +1 frameshifting occurs has not been fully understood. Here, taking Escherichia coli RF2 programmed frameshifting as an example we present systematical analysis of the +1 frameshifting that could occur during every state-transition step in ...
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Cost minimization of ribosomal frameshifts
Journal of Theoretical Biology, 2007Properties of mRNA leading regions that modulate protein synthesis are little known (besides effects of their secondary structure). Here I explore how coding properties of leading regions may account for their disparate efficiencies. Trinucleotides that form off frame stop codons decrease costs of ribosomal slippages during protein synthesis: protein ...
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An ‘integrated model’ of programmed ribosomal frameshifting
Trends in Biochemical Sciences, 2002Many viral mRNAs, including those of HIV-1, can make translating ribosomes change reading frame. Altering the efficiencies of programmed ribosomal frameshift (PRF) inhibits viral propagation. As a new target for potential antiviral agents, it is therefore important to understand how PRF is controlled.
Jason W, Harger +2 more
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Leftward ribosome frameshifting at a hungry codon
Journal of Molecular Biology, 1992Previous experiments have shown that limitation for certain aminoacyl-tRNA species results in phenotypic suppression of a subset of frameshift mutant alleles, including members in both the (+) and (-) incorrect reading frames. Here, we demonstrate that such phenotypic suppression can occur through a ribosome reading frame shift at a hungry AAG codon ...
J A, Gallant, D, Lindsley
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Enhanced Ribosome Frameshifting in Stationary Phase Cells
Journal of Molecular Biology, 1996We have examined the effect of growth phase in Escherichia coli on the translation of a plasmid-borne lacZ gene in which active enzyme synthesis requires a leftward frameshift. During the log phase of growth, the differential rate of enzyme synthesis is very low. It increases by about two orders of magnitude during the small amount of protein synthesis
Z, Barak +4 more
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Ribosomal Frameshifting from -2 to +50 Nucleotides
1990Publisher Summary This chapter describes ribosomal frame-shifting from the mRNA's point of the view. Ribosomes can obviously progress in a stately and efficient manner through coding sequences without being unduly worried about losing their way. In some cases, these sites of “programmed” ribosomal frame-shifting serve to control dynamically the level
R B, Weiss +3 more
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Programmed −1 ribosomal frameshifting in the SARS coronavirus
Biochemical Society Transactions, 2004Programmed −1 ribosomal frameshifting is an alternate mechanism of translation used by coronavirus to synthesize replication proteins encoded by two overlapping open reading frames. For some coronaviruses, the mRNA cis-acting stimulatory structures involved in this process have been characterized, but their precise contribution to ribosomal ...
F, Dos Ramos +3 more
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Hungry Codons Promote Frameshifting in Human Mitochondrial Ribosomes
Science, 2010During translation of mitochondrial genes, shifting the ribosome reading frame avoids unconventional arginine codons.
Temperley R +4 more
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Structural basis of ribosomal frameshifting
2021Versatility in genetic decoding enriches gene expression. Programmed shifting of the reading frame during translation is a prominent feature productively utilized in probably all life forms. While the occurrences, function, and mechanistic components have been identified for many instances of frameshifting, structural understanding of their basis at ...
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