Results 1 to 10 of about 59,550 (145)

CRISPR screening reveals a dependency on ribosome recycling for efficient SARS-CoV-2 programmed ribosomal frameshifting and viral replication [PDF]

Cell Reports, 2023
Summary: During translation of the genomic RNA of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative virus in the COVID-19 pandemic, host ribosomes undergo programmed ribosomal frameshifting (PRF) at a conserved structural ...
Frederick Rehfeld, Jennifer L. Eitson, Maikke B. Ohlson, Tsung-Cheng Chang, John W. Schoggins, Joshua T. Mendell   +5 more
doaj   +2 more sources

Programmable modulation of ribosomal frameshifting by mRNA targeting CRISPR-Cas12a system [PDF]

iScience, 2023
Summary: Minus 1 programmed ribosomal frameshifting (−1 PRF) is a conserved translational regulation event essential for critical biological processes, including the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication.
Shih-Hong Huang, Shih-Cheng Chen, Tsu-Ying Wu, Cheng-Yao Chen, Chien-Hung Yu   +4 more
doaj   +2 more sources

Ribosomal frameshifting used in influenza A virus expression occurs within the sequence UCC_UUU_CGU and is in the +1 direction [PDF]

Open Biology, 2012
Programmed ribosomal frameshifting is used in the expression of many virus genes and some cellular genes. In eukaryotic systems, the most well-characterized mechanism involves –1 tandem tRNA slippage on an X_XXY_YYZ motif.
A. E. Firth, B. W. Jagger, H. M. Wise, C. C. Nelson, K. Parsawar, N. M. Wills, S. Napthine, J. K. Taubenberger, P. Digard, J. F. Atkins   +9 more
doaj   +4 more sources

Thinking Outside the Frame: Impacting Genomes Capacity by Programmed Ribosomal Frameshifting [PDF]

Frontiers in Molecular Biosciences, 2022
Translation facilitates the transfer of the genetic information stored in the genome via messenger RNAs to a functional protein and is therefore one of the most fundamental cellular processes.
Ricarda J. Riegger, Ricarda J. Riegger, Neva Caliskan, Neva Caliskan   +3 more
doaj   +2 more sources

Protein-inducible ribosomal frameshifting enables programmable translational control for genetic circuit design in Escherichia coli [PDF]

Journal of Biological Engineering
Background Programmed ribosomal frameshifting (PRF) is a translational mechanism that enables the ribosome to shift reading frames and access alternative coding sequences.
Seongho Hong, Yelin Lim, Hansol Kang, Jongmin Kim   +3 more
doaj   +2 more sources

Lycorine Derivative Inhibits SARS-CoV-2 Replication by Reducing -1 Programmed Ribosomal Frameshifting via Targeting ZAP. [PDF]

MedComm (2020)
The present study evaluated the antiviral potential of various lycorine derivatives and identified compound 7 as a promising candidate. Compound 7 demonstrated strong antiviral efficacy against both the original SARS‐CoV‐2 and its variants in vitro and in vivo.
Du T, Liu R, Zhang X, Shen L, Tang C, Wang J, Cheng Y, Yu W, Yin B, Lu S, Pan X, Peng X.   +11 more
europepmc   +2 more sources

Evolutionary origin of the frameshift sites in the ribosomal frameshifting genes of Euplotes [PDF]

BMC Genomics
Background Programmed ribosome frameshifting is a translational recoding event in which ribosomes slip forward or backward along the mRNA. Although genes utilizing programmed ribosomal frameshifting for their expression have been found in most organisms,
Ruan-lin Wang, Xiao-yan Liu, Qing-yao Meng, Xin Zhang, Yue-jun Fu, Ai-hua Liang   +5 more
doaj   +2 more sources

Programmed −1 ribosomal frameshifting from the perspective of the conformational dynamics of mRNA and ribosomes [PDF]

Computational and Structural Biotechnology Journal, 2021
Programmed −1 ribosomal frameshifting (−1 PRF) is a translation mechanism that regulates the relative expression level of two proteins encoded on the same messenger RNA (mRNA).
Kai-Chun Chang, Jin-Der Wen
doaj   +2 more sources

Abracadabra, One Becomes Two: The Importance of Context in Viral −1 Programmed Ribosomal Frameshifting [PDF]

mBio, 2022
The constrained nature of viral genomes has allowed a translational sleight of hand known as −1 Programmed Ribosomal Frameshifting (−1 PRF) to flourish. Numerous studies have sought to tease apart the mechanisms and implications of −1PRF utilizing a few ...
Wesley D. Penn, Suchetana Mukhopadhyay
doaj   +2 more sources

Accounting for Programmed Ribosomal Frameshifting in the Computation of Codon Usage Bias Indices [PDF]

G3: Genes, Genomes, Genetics, 2018
Experimental evidence shows that synonymous mutations can have important consequences on genetic fitness. Many organisms display codon usage bias (CUB), where synonymous codons that are translated into the same amino acid appear with distinct frequency ...
Victor Garcia, Stefan Zoller, Maria Anisimova   +2 more
doaj   +3 more sources