Results 41 to 50 of about 59,620 (205)

Structural and molecular basis for Cardiovirus 2A protein as a viral gene expression switch

Nature Communications, 2021
Many RNA viruses employ programmed –1 ribosomal frameshifting (PRF) to expand their coding capacity and optimize production of viral proteins. Here, the authors report structural and biophysical analysis of protein 2A from a cardiovirus, with insights ...
Chris H. Hill, Lukas Pekarek, Sawsan Napthine, Anuja Kibe, Andrew E. Firth, Stephen C. Graham, Neva Caliskan, Ian Brierley   +7 more
doaj   +1 more source

Modulation of Viral Programmed Ribosomal Frameshifting and Stop Codon Readthrough by the Host Restriction Factor Shiftless

Viruses, 2021
The product of the interferon-stimulated gene C19orf66, Shiftless (SHFL), restricts human immunodeficiency virus replication through downregulation of the efficiency of the viral gag/pol frameshifting signal.
Sawsan Napthine, Chris H. Hill, Holly C. M. Nugent, Ian Brierley   +3 more
doaj   +1 more source

Novel viruses of the genus Amalgavirus from crown imperial Fritillaria imperialis transcriptome [PDF]

Journal of Crop Protection, 2023
In an in silico investigation, genome sequences of three RNA viruses were identified in the crown imperial Fritillaria imperialis L. transcriptome dataset.
Sajad Astaraki, Masoud Shams-bakhsh
doaj   +1 more source

N¹-methylpseudouridylation of mRNA causes +1 ribosomal frameshifting. [PDF]

Nature, 2023
AbstractIn vitro-transcribed (IVT) mRNAs are modalities that can combat human disease, exemplified by their use as vaccines for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). IVT mRNAs are transfected into target cells, where they are translated into recombinant protein, and the biological activity or immunogenicity of the encoded ...
Mulroney TE, Pöyry T, Yam-Puc JC, Rust M, Harvey RF, Kalmar L, Horner E, Booth L, Ferreira AP, Stoneley M, Sawarkar R, Mentzer AJ, Lilley KS, Smales CM, von der Haar T, Turtle L, Dunachie S, Klenerman P, Thaventhiran JED, Willis AE.   +19 more
europepmc   +6 more sources

Ribosomal frameshifting in yeast viruses [PDF]

Yeast, 1995
AbstractProper maintenance of translational reading frame by ribosomes is essential for cell growth and viability. In the last 10 years it has been shown that a number of viruses induce ribosomes to shift reading frame in order to regulate the expression of gene products having enzymatic functions. Studies on ribosomal frameshifting in viruses of yeast
openaire   +2 more sources

Loss of N1-methylation of G37 in tRNA induces ribosome stalling and reprograms gene expression

eLife, 2021
N1-methylation of G37 is required for a subset of tRNAs to maintain the translational reading-frame. While loss of m1G37 increases ribosomal +1 frameshifting, whether it incurs additional translational defects is unknown.
Isao Masuda, Jae-Yeon Hwang, Thomas Christian, Sunita Maharjan, Fuad Mohammad, Howard Gamper, Allen R Buskirk, Ya-Ming Hou   +7 more
doaj   +1 more source

Transcription-translation coupling: direct interactions of RNA polymerase with ribosomes and ribosomal subunits. [PDF]

, 2017
In prokaryotes, RNA polymerase and ribosomes can bind concurrently to the same RNA transcript, leading to the functional coupling of transcription and translation.
Blaha, Gregor M, Conn, Adam B, Diggs, Stephen, Fan, Haitian, Gamper, Howard B, Hahm, Joseph, Hou, Ya-Ming, O'Leary, Seán E, Wang, Yinsheng, Williams, Preston B   +9 more
core   +1 more source

Altering SARS Coronavirus Frameshift Efficiency Affects Genomic and Subgenomic RNA Production

Viruses, 2013
In previous studies, differences in the amount of genomic and subgenomic RNA produced by coronaviruses with mutations in the programmed ribosomal frameshift signal of ORF1a/b were observed.
Ewan P. Plant, Deborah R. Taylor, Jonathan D. Dinman, Ralph S. Baric, Amy C. Sims   +4 more
doaj   +1 more source

Characterization of Ribosomal Frameshifting in Theiler's Murine Encephalomyelitis Virus. [PDF]

, 2015
Theiler's murine encephalomyelitis virus (TMEV) is a member of the genus Cardiovirus in the Picornaviridae, a family of positive-sense single-stranded RNA viruses. Previously, we demonstrated that in the related cardiovirus, Encephalomyocarditis virus, a
Bell, Susanne, Brierley, Ian, Finch, Leanne K, Firth, Andew E, Lardinois, Cécile, Ling, Roger, Loughran, Gary, Michiels, Thomas, Napthine, Sawsan, Olspert, Allan   +9 more
core   +2 more sources

Identification of the nature of reading frame transitions observed in prokaryotic genomes [PDF]

, 2013
Our goal was to identify evolutionary conserved frame transitions in protein coding regions and to uncover an underlying functional role of these structural aberrations.
Antonov, Ivan, Atkins, John F., Baranov, Pavel V., Borodovsky, Mark, Coakley, Arthur   +4 more
core   +1 more source