FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Effects of Probiotic Supplementation from Dry-Off Through Early Lactation on Serum Protein Indices and Free Amino Acids in Dairy Cows: A Randomized Field Study. [PDF]
Int J Mol SciMarczuk J +5 more
europepmc +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Dynamic migration of free amino acids from garlic in light soy sauce and its impact on taste formation. [PDF]
Front NutrLan ML +5 more
europepmc +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Effects of <i>Allium mongolicum</i> Essential Oil on Meat Quality and Flavor-Related Free Amino Acids in Finishing Lambs. [PDF]
Animals (Basel)Erdene K +6 more
europepmc +1 more source
Copper toxicity leads to accumulation of free amino acids and polyphenols in Phaeodactylum tricornutum diatoms. [PDF]
Environ Sci Pollut Res Int, 2023 Santiago-Díaz P +5 more
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
FEBS Open Bio, EarlyView.We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Investigation on the dynamic change of key odor compounds, free amino acids and chroma during soy sauce fermentation. [PDF]
Food Chem XZhou Y +6 more
europepmc +1 more source
Contribution of Phenolics and Free Amino Acids on the Antioxidant Profile of Commercial Lemon Verbena Infusions. [PDF]
Antioxidants (Basel), 2023 Peixoto JAB +7 more
europepmc +1 more source