Results 61 to 70 of about 229,386 (310)

Cell‐cycle‐specific lesion evolution rather than inhibition of double‐strand‐break repair underpins cisplatin radiosensitization

Molecular Oncology, EarlyView.
We analyze cisplatin–DNA adducts (CDAs) and double‐strand breaks (DSBs) in a cell‐cycle‐dependent manner. We find that CDAs form similarly across all cell cycle phases. DSBs arise only in S‐phase. CDAs might not directly impair DSB repair, but S‐phase DSB lesions evolve in the presence of CDAs and disrupt repair in G2, also causing radiosensitization ...
Ye Qiu, Xixi Lin, Emil Mladenov, Veronika Mladenova, Jürgen Thomale, Ali Sak, Yao Wang, Yunxuan Deng, Eleni Gkika, Martin Stuschke, George Iliakis   +10 more
wiley   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

Barbaloin content of aloe (Aloe barbadensis) leaf exudates as affected by different drying techniques

The Indian Journal of Agricultural Sciences, 2015
Aloe (Aloe barbadensis Mill.) is commercially cultivated for its transparent leaf gel and leaf exudates. The leaf exudates collected from epidermal layer contain anthraquinone glycosides (aloins) mainly barbaloin (aloin A) and isobarbaloin (aloin B ...
RAJU SARAVANAN, NARENDRA ATMARAM GAJBHIYE, JAYANTI S MAKASANA, VELUMANI RAVI   +3 more
doaj   +1 more source

Influence of drying treatment on the physical and chemical properties of cucumber. [PDF]

, 2011
The present study evaluates the effect of drying treatments such as convective air drying and freeze-drying on the colour, texture, chemical properties (ash, sugars, fibre and vitamin C), phenolic compounds and antioxidant activity of ...
Barroca, Maria João, Guiné, Raquel, Henriques, Francisca   +2 more
core  

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

BMI‐1 modulation and trafficking during M phase in diffuse intrinsic pontine glioma

FEBS Open Bio, EarlyView.
The schematic illustrates BMI‐1 phosphorylation during M phase, which triggers its translocation from the nucleus to the cytoplasm. In cycling cells, BMI‐1 functions within the PRC1 complex to mediate H2A K119 monoubiquitination. Following PTC596‐induced M phase arrest, phosphorylated BMI‐1 dissociates from PRC1 and is exported to the cytoplasm via its
Banlanjo Umaru, Deepak Kumar Mishra, Shiva Senthil Kumar, Hao‐Han Pang, Brendan Devine, Komal Khan, Rachid Drissi   +6 more
wiley   +1 more source

Intensification of the freeze drying process by the control of both freezing and primary drying steps [PDF]

, 2013
The problem of optimization of freeze-drying cycles is addressed, with emphasis in both freezing and primary drying steps. In particular, this study shows that the control of the nucleation event produces more uniform batches (as ice nucleation is ...
Barresi, Antonello, Bullich, R., Oddone, Irene, Pisano, Roberto   +3 more
core  

Effect of freeze-drying and gamma irradiation on biomechanical properties of bovine pericardium [PDF]

, 2005
Freeze-drying and gamma irradiation are the techniques widely use in tissue banking for preservation and sterilization of tissue grafts respectively.
Abu Bakar @ Zakaria, Md Zuki, Hassan, Asnah, Mohamad Yusof, Loqman, Mohamed Mustapha, Noordin, Mohd Yusof, Ainul Yuzairi, Yagoub Mohamed, Abdel Hafeez, Yusof, Norimah   +6 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Human Sperm Preservation Using Freeze-drying an Experimental Laboratory Study [PDF]

, 2010
Latar Belakang: Terdapat beberapa teknik preservasi sperma yang masing-masing memiliki kelebihan dan kekurangan. Salah satu alternatif tekniknya adalah dengan teknik pengeringan. Teknik ini bersifat lebih murah, mudah, dan praktis.
I'tishom, R. (Reny), Lunardhi, H. (Hamdani), Pramesti, M. B. (MP)   +2 more
core