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Freeze-fracture-autoradiography
Histochemistry, 1976A new method for the electron microscope autoradiography of soluble substances in frozen tissue is described. The basic features of the method are freeze fracturing, the application of a suitable monolayer followed by exposure at low temperature and finally the separation of tissue and the replica-monolayer-sandwich after photographic processing.
E, Rix, A, Schiller, R, Taugner
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Complementary freeze‐fracture, freeze‐etch specimens
Journal of Microscopy, 1979SUMMARYA procedure is described for the preparation and comparison of complementary freeze‐fracture‐freeze‐etch specimens. These complementary replicas reveal the value of etching some specimens even when the cryoprotectant concentration amounts to as much as 25% glycerol and 25% sucrose.
R L, Steere, E F, Erbe
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Freeze-fracture immunogold labeling
Histochemistry and Cell Biology, 1996Several approaches have been developed to combine immunogold cytochemistry and freeze-fracture techniques. These methods are highly heterogeneous regarding both the sequence of the procedural steps and the aspect of the resulting images. They imply immunolabeling either before or after freeze-fracture or even immunolabeling of platinum/carbon replicas ...
TORRISI, Maria Rosaria +1 more
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Freeze Fracture and Freeze Etching
2013Freeze fracture depends on the property of frozen tissues or cells, when cracked open, to split along the hydrophobic interior of membranes, thus revealing broad panoramas of membrane interior. These large panoramas reveal the three-dimensional contours of membranes making the methods well suited to studying changes in membrane architecture.
Douglas E, Chandler, William P, Sharp
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Journal of Electron Microscopy, 1996
We present a novel freeze-fracture cytochemistry method based upon enzyme cytochemistry. By this method, freeze-fractured membranes are labeled with cerium as an enzyme cytochemical marker on replicas. The cerium capture method was suitable for freeze-fracture enzyme cytochemistry because the cerium phosphate reaction product is stable after replica ...
T, Takizawa, T, Saito
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We present a novel freeze-fracture cytochemistry method based upon enzyme cytochemistry. By this method, freeze-fractured membranes are labeled with cerium as an enzyme cytochemical marker on replicas. The cerium capture method was suitable for freeze-fracture enzyme cytochemistry because the cerium phosphate reaction product is stable after replica ...
T, Takizawa, T, Saito
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Freeze-fracture electron microscopy
Nature Protocols, 2007The freeze-fracture technique consists of physically breaking apart (fracturing) a frozen biological sample; structural detail exposed by the fracture plane is then visualized by vacuum-deposition of platinum-carbon to make a replica for examination in the transmission electron microscope.
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Complementary freeze-fracture and freeze-etch preparations
Proceedings, annual meeting, Electron Microscopy Society of America, 1984Whereas freeze-etching technology was introduced in 1957 (1) and the first freeze-etching equipment to be made available commercially was developed in 1961 (2), the need for complementary replicas was not recognized until Branton (3) proposed that the two membrane faces seen in freeze-etched specimens were not the inner and outer surfaces of membranes ...
Russell L. Steere +2 more
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Combined Freeze-Fracturing and Autoradiography Techniques: Freeze-Fracture Autoradiography
1981In the past many efforts have been made to apply classical techniques of fixation and embedding to the autoradiography of soluble material. There is general agreement today that this goal can only be reached by cryotechniques (Amlacher 1974).
A. Schiller, R. Taugner
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A simple and reliable quick-freezing/freeze-fracturing procedure
Histochemistry and Cell Biology, 1997We describe a simple method for the quick-freezing/freeze-fracturing of cells in tissues or culture monolayers. Tissue slices or cultured cells were covered with thin copper foil (10-micron-thick), and frozen by smashing them against a liquid helium-cooled copper block.
K, Fujimoto, T, Noda, T, Fujimoto
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