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Freeze Fracture of Intact Plant Tissues

Stain Technology, 1982
A procedure for dissolving and handling replicas of cutinized leaves and other plant tissues is described. This technique yields comparatively large replicas which can include vascular tissue, epidermal cells and the cuticle. Dissolution of the tissue involves the sequential use of alcoholic KOH, sulfuric acid and chromic acid.
K A, Platt-Aloia, W W, Thomson
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Freeze‐fracture of manufactured foods

Journal of Electron Microscopy Technique, 1989
AbstractManufacture of food products with desired texture and stability increasingly benefits from an understanding of the food microstructure. Freeze‐fracture is advantageous in gaining this understanding because it avoids alterations of hydration, retains fat, and maximally preserves air cells.
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Hodgkin cells in freeze-fracture replicas

Virchows Archiv B Cell Pathology, 1988
Lymph nodes from six patients with Hodgkin's disease (three with the nodular sclerosing subtype, one with mixed cellularity and two with the lymphocyte-predominant subtype) were analysed by electron microscopy in freeze-fracture replicas and thin sections.
E, Kaiserling, H, Wolburg, K, Berg
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Freeze‐fracture: A personal history

Journal of Electron Microscopy Technique, 1989
AbstractThe sequence of events leading to the development of freeze‐fracture replication is described. Subsequent developments discussed include complementary replicas, replica interpretation with stereo micrograph and reversal negatives, replica reinforcement, and control of resistance evaporation.
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Freeze-fracture of adrenal chromaffin granules

Neuroscience, 1977
Abstract Freeze-fracture replicas of bovine adrenal chromaffin granules show convex and concave fracture faces. The concave fracture faces are studded with 9 nm diameter particles (on average 950/μm 2 ) while the convex fracture faces are almost devoid of particles (approx 150/μm 2 ).
P A, Eagles   +3 more
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Freeze-fracture of intact sarcotubular membranes

Journal of Ultrastructure Research, 1978
Pectoralis muscle from normal and dystrophic chickens was examined following preparation by the technique of freeze-fracturing. The intramembranous particle density of both the cytoplasmic and extracellular (luminal) faces of the junctional sarcoplasmic reticulum (SR), free SR, and T system of normal and dystrophic chickens was determined and subjected
L M, Crowe, R J, Baskin
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Freeze-fracture morphology of nuclear pockets

Virchows Archiv B Cell Pathology Including Molecular Pathology, 1991
Nuclear pockets (NP) are found in numerous human tumours and in certain non-neoplastic cells. This study concerns the structure of NP in cells from two malignant rhabdoid tumours, one embryonal rhabdomyosarcoma, two centroblastic/centrocytic lymphomas, one centrocytic lymphoma and one Ki-1 lymphoma, as well as in normal neutrophils.
E, Kaiserling, H, Wolburg
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Freeze-fracture studies on tardigrade cuticle

Tissue and Cell, 1983
The cuticles of the heterotardigrade Echiniscus testudo and the eutardigrades Macrobiotus hufelandi and Milnesium tardigradum have been studied using freeze-fracture technique. Most of the layers seen in conventional TEM micrographs can be visualized. There is no clear evidence that the trilaminar components of the cuticle such as the outer epicuticle ...
H, Greven, H, Robenek
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Freeze-Etching and Freeze-Fracturing

1973
The freeze-etching (or fracturing) technique involves the making of a platinum-carbon replica of the fracture face through frozen cells. This replica is examined in the electron microscope. There are two advantages compared with earlier techniques. Firstly, fixation and dehydration are not necessary and the replica is thus of cells which differ from ...
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Freeze‐fracturing and freeze‐etching of cardiac myosin filaments

Journal of Microscopy, 1975
SUMMARYMyofilament structure was studied in freeze‐etch replicas of unfixed, glycerinated beef cardiac muscle. The information which is revealed depends upon the direction of metal shadowing in relation to the filament axis. Shadows oblique to this axis reveal that the outer surface of a longitudinal half of a thick filament comprises three, sometimes ...
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