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Freeze‐fracture for scanning electron microscopy

Journal of Microscopy, 1977
SUMMARYTwo different freeze‐fracture methods are explored for preparation of biological material for scanning electron microscopy. In the simpler method the tissues are first fixed and dehydrated. They are then frozen and fractured, and after thawing, critical‐point dried.
G H, Haggis, B, Phipps-Todd
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Freeze‐fracture observations on mammalian oocytes

American Journal of Anatomy, 1985
AbstractFreeze‐fracture studies on mammalian oocytes have been hampered by the relatively small numbers of cells available at a given time as well as by difficulties encountered in effectively freezing these large, watery cells. We have nevertheless pursued this area because of the benefits of visualizing membrane faces involved in various fusion ...
J K, Koehler, J M, Clark, D, Smith
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Freeze-fracture techniques

1991
Abstract Freeze-fracture techniques were developed originally to prepare cellular ultrastructures in a frozen-hydrated and living state for electron microscopy (I). The replicas made from freeze-fractured biological specimens have provided a new three-dimensional ultrastructure of cells (2).
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Freeze-Fracture of Circulating Human Eosinophils

Acta Haematologica, 1977
Freeze-fracture has been carried out on peripheral blood from three patients with eosinophilia. Eosinophil granule membranes are characterized by intramembranous particles (IMP) with diameters of 80-150 A. These particles of the granule membrane are consistently larger than the IMP of the plasma membrane which are approximately 80 A in diameter.
S D, Douglas, M P, Ooka
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Freeze-Fracture Transmissionselektronenmikroskopie

2017
Der zweite Teil der vorliegenden Masterarbeit befasst sich mit der Untersuchung von drei Flussigkristallen mittels Freeze-Fracture Transmissionselektronenmikroskopie (FFTEM). Die untersuchten Mesogene sind in Tabelle 5.1 aufgelistet. Die Technik der Freeze-Fracture Transmissionselektronenmikroskopie wurde ursprunglich fur biologische Systeme entwickelt
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Freeze‐fracture cytochemistry: Review of methods

Journal of Electron Microscopy Technique, 1989
Abstract“Freeze‐fracture cytochemistry” encompasses a diversity of recently developed techniques in which freeze‐fracture and cytochemistry are combined. Cytochemical labeling may, in principle, be integrated into one of three basic points in the standard freeze‐fracture procedure; (1) before the specimen is frozen, (2) after it has been fractured, or (
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Freeze-fracturing of dispersions.

Microscopica acta, 1977
In the last few years freeze-fracturing has proved to be a useful method for the investigation of dispersions. The freeze-fracture technique provides, above all, information on the arrangement of the colloidal particles in the liquid medium (e.g. on the state of agglomeration). Furthermore, it leads to conclusions about the structure, shape and size of
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Preparing Hydra for Freeze-Fracture and Freeze-Etching

1983
The freeze-fracture technique is particularly advantageous for the study of cell surfaces and intercellular junctions. In general, it involves the rapid freezing of tissues, either with or without partial sublimation of ice (etching). By evaporating a film of pure carbon on the exposed surface, a replica of the cell surface is obtained which reveals ...
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