Results 131 to 140 of about 876,006 (355)

Homologous expression and purification of human HAX‐1 for structural studies

open access: yesFEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Dietary nitrate and nitrite protect against doxorubicin‐induced cardiac fibrosis and oxidative protein damage in tumor‐bearing mice

open access: yesFEBS Open Bio, EarlyView.
Chemotherapies such as doxorubicin can have toxic effects on healthy cardiovascular/heart tissue. Following up on a doxorubicin toxicity study in mice without tumors where nitrate water was cardioprotective (lessened toxicity), this study with tumor‐bearing mice undergoing doxorubicin treatment showed no negative effect of nitrate and nitrite on drug ...
Rama D. Yammani   +7 more
wiley   +1 more source

Lipid-based Transfection Reagents Exhibit Cryo-induced Increase in Transfection Efficiency

open access: yesMolecular Therapy: Nucleic Acids, 2016
The advantages of lipid-based transfection reagents have permitted their widespread use in molecular biology and gene therapy. This study outlines the effect of cryo-manipulation of a cationic lipid-based formulation, Lipofectamine 2000, which, after ...
Helena Sork   +17 more
doaj   +1 more source

Fuel system technology overview [PDF]

open access: yes, 1980
Fuel system research and technology studies are being conducted to investigate the correlations and interactions of aircraft fuel system design and environment with applicable characteristics of the fuel.
Friedman, R.
core   +1 more source

Freezing Transition of Compact Polyampholytes

open access: yes, 1996
Polyampholytes (PAs) are heteropolymers with long range Coulomb interactions. Unlike polymers with short range forces, PA energy levels have non-vanishing correlations and are thus very different from the Random Energy Model (REM).
Grosberg, Alexander Yu.   +4 more
core   +1 more source

Naked cuticle is essential for Drosophila wing development beyond Wingless signaling

open access: yesFEBS Open Bio, EarlyView.
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley   +1 more source

Rheological, sensory, and microstructural properties of fresh and frozen/thawed mashed potatoes enriched with different proteins

open access: yesCyTA - Journal of Food, 2018
In this study, the effect of the addition of soybean protein isolate (SPI), whey protein isolate (WPI), whole milk powder (WMP), and sodium caseinate (SC) on the rheological, physical, sensory, and structural properties of fresh and frozen/thawed mashed ...
Wenhua Miao   +5 more
doaj   +1 more source

Theory of Coexisting Transverse Spin Freezing and Long-Ranged Antiferromagnetic Order in Lightly Doped La_{2-x}Sr_x CuO_4

open access: yes, 1993
We provide an explanation of the spin-freezing transition recently observed by Chou et al. (Phys. Rev. Lett.
Gooding, R. J.   +2 more
core   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

open access: yesFEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso   +4 more
wiley   +1 more source

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