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European Journal of Obstetrics & Gynecology and Reproductive Biology, 2004
Blastocyst are preimplantation embryos that have successfully passed the critical step of genomic activation and have so a high developmental potential, thus allowing to reduce the number of embryos transferred. Blastocyst culture and freezing have really started in IVF programs in the early nineties, when it was possible to obtain good blastocysts ...
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Blastocyst are preimplantation embryos that have successfully passed the critical step of genomic activation and have so a high developmental potential, thus allowing to reduce the number of embryos transferred. Blastocyst culture and freezing have really started in IVF programs in the early nineties, when it was possible to obtain good blastocysts ...
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Complementary freeze‐fracture, freeze‐etch specimens
Journal of Microscopy, 1979SUMMARYA procedure is described for the preparation and comparison of complementary freeze‐fracture‐freeze‐etch specimens. These complementary replicas reveal the value of etching some specimens even when the cryoprotectant concentration amounts to as much as 25% glycerol and 25% sucrose.
R L, Steere, E F, Erbe
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Journal of Microscopy, 1982
SUMMARYFreeze‐substitution is a technique suitable for the preparation of unicellular and multicellular plant and animal specimens for conventional light microscopy, TEM and SEM. It is also widely used as a means of preparing animal and plant tissues for the localization of water soluble substances by analytical electron microscopy, autoradiography or ...
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SUMMARYFreeze‐substitution is a technique suitable for the preparation of unicellular and multicellular plant and animal specimens for conventional light microscopy, TEM and SEM. It is also widely used as a means of preparing animal and plant tissues for the localization of water soluble substances by analytical electron microscopy, autoradiography or ...
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Seminars in Reproductive Medicine, 2001
Human oocyte cryopreservation is potentially an alternative solution to the ethical problems arising from embryo storage. In addition, oocyte freezing is the only method to preserve the reproductive capacity for women at risk of losing it because of premature ovarian failure, pelvic diseases, surgery, or antineoplastic treatments.
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Human oocyte cryopreservation is potentially an alternative solution to the ethical problems arising from embryo storage. In addition, oocyte freezing is the only method to preserve the reproductive capacity for women at risk of losing it because of premature ovarian failure, pelvic diseases, surgery, or antineoplastic treatments.
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2012
Fil: Martino, Miriam Nora. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos.
Ramallo, Laura Ana +2 more
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Fil: Martino, Miriam Nora. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos.
Ramallo, Laura Ana +2 more
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Freezing and freeze-drying of microorganisms
Cryobiology, 1964Some papers reporting the effects of intense cold on microorganisms had already appeared in the 19th century and since then there have been a great many investigations of the freezing of microorganisms.3* 53 ‘I, 12 These experiments demonstrate that a wide variety of microorganisms can survive cooling to and storage at very low temperatures.
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Freeze‐drying using vacuum‐induced surface freezing
Journal of Pharmaceutical Sciences, 2002A method of freezing during freeze-drying, which avoids undercooling of a solution and allows growth of large, dendritic ice crystals, was investigated. Aqueous solutions of mannitol, sucrose, or glycine were placed under a chamber vacuum of approximately 1 mbar at a shelf temperature of +10 degrees C.
Martin, Kramer +2 more
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Proceedings, annual meeting, Electron Microscopy Society of America, 1968
Freeze-etching, as a tool in the preparation of biological specimens for electron microscopy, has seen relatively little use since it was introduced in 1957. The involved procedure, originally described, and, until recently, the cost of special equipment, must certainly have played a part in the failure of freeze-etching to become a commonly used ...
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Freeze-etching, as a tool in the preparation of biological specimens for electron microscopy, has seen relatively little use since it was introduced in 1957. The involved procedure, originally described, and, until recently, the cost of special equipment, must certainly have played a part in the failure of freeze-etching to become a commonly used ...
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Freezing and Freeze-Drying of Bull Spermatozoa
American Journal of Physiology-Legacy Content, 1957The possible dependance of survival upon the time spermatozoa are in contact with the protective substance glycerol, prior to exposure to low temperatures, was investigated. Pretreatment for 5 minutes provided at least as much protection as 18 hours.
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Canning, Freezing, and Freeze-Drying
1991Rice (Oryza sativa) is a staple grain consumed by more than half of the world’s population (Hogan 1977). It is the chief source of calories in Asia and has become more popular in Western countries in recent years (Burns and Gerdes 1985). Common objections to using rice in Western countries include difficulty and length of preparation and established ...
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