Results 11 to 20 of about 229,738 (330)

In-Vitro Characterization of mCerulean3_mRuby3 as a Novel FRET Pair with Favorable Bleed-Through Characteristics

Biosensors, 2019
In previous studies, we encountered substantial problems using the CFP_YFP Förster resonance energy transfer (FRET) pair to analyze protein proximity in the endoplasmic reticulum of live cells.
Kira Erismann-Ebner, Anne Marowsky, Michael Arand   +2 more
doaj   +2 more sources

Application of FRET Biosensors in Mechanobiology and Mechanopharmacological Screening

Frontiers in Bioengineering and Biotechnology, 2020
Extensive studies have shown that cells can sense and modulate the biomechanical properties of the ECM within their resident microenvironment. Thus, targeting the mechanotransduction signaling pathways provides a promising way for disease intervention ...
Longwei Liu, Fangchao He, Yiyan Yu, Yingxiao Wang   +3 more
doaj   +2 more sources

A Guide to Fluorescent Protein FRET Pairs

Sensors, 2016
Förster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution. Fluorescent proteins (FPs) are most
Bryce T. Bajar, Emily S. Wang, Shu Zhang, Michael Z. Lin, Jun Chu   +4 more
doaj   +2 more sources

Unraveling multi-state molecular dynamics in single-molecule FRET experiments. I. Theory of FRET-lines [PDF]

Journal of Chemical Physics, 2021
Conformational dynamics of biomolecules are of fundamental importance for their function. Single-molecule studies of Förster Resonance Energy Transfer (smFRET) between a tethered donor and acceptor dye pair are a powerful tool to investigate the ...
Anders Barth, O. Opanasyuk, Thomas O. Peulen, S. Felekyan, S. Kalinin, H. Sanabria, C. Seidel   +6 more
semanticscholar   +1 more source

FRET Based Biosensor: Principle Applications Recent Advances and Challenges

Diagnostics, 2023
Förster resonance energy transfer (FRET)-based biosensors are being fabricated for specific detection of biomolecules or changes in the microenvironment.
A. K. Verma, Ashab Noumani, A. K. Yadav, P. Solanki   +3 more
semanticscholar   +1 more source

Advances in FRET‐based biosensors from donor‐acceptor design to applications

Aggregate, 2023
Fluorogenic biosensors are essential tools widely used in biomedicine, chemical biology, environmental protection and food safety. Fluorescence resonance energy transfer (FRET) is a crucial technique for developing fluorogenic biosensors that provide ...
Wen-Chao Yang, Shuang-Yu Li, Shengnan Ni, Guozhen Liu   +3 more
semanticscholar   +1 more source

Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

bioRxiv, 2022
An international blind study confirms that smFRET measurements on dynamic proteins are highly reproducible across instruments, analysis procedures and timescales, further highlighting the promise of smFRET for dynamic structural biology.
G. Agam, C. Gebhardt, Milana Popara, Rebecca Mächtel, Julian Folz, Benjamin Ambrose, Neharika Chamachi, SangYoon Chung, Timothy D. Craggs, Marijn de Boer, Dina Grohmann, T. Ha, Andreas Hartmann, Jelle Hendrix, Verena Hirschfeld, C. Hübner, T. Hugel, Dominik Kammerer, Hyun-Seo Kang, A. Kapanidis, G. Krainer, Kevin Kramm, E. Lemke, E. Lerner, E. Margeat, Kristen Martens, Jens Michaelis, Jaba Mitra, G. G. Moya Muñoz, Robert E Quast, Nicole Robb, M. Sattler, M. Schlierf, J. Schneider, Tim Schröder, Anna Sefer, P. S. Tan, Johann Thurn, Philip Tinnefeld, J. van Noort, S. Weiss, Nicolas D. Wendler, N. Zijlstra, Anders Barth, C. Seidel, D. Lamb, T. Cordes   +46 more
semanticscholar   +1 more source

Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study [PDF]

Nature Methods, 2017
Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo.
Björn Hellenkamp, S. Schmid, O. Doroshenko, O. Opanasyuk, R. Kühnemuth, Soheila Rezaei Adariani, Benjamin Ambrose, M. Aznauryan, Anders Barth, V. Birkedal, M. Bowen, Hongtao Chen, T. Cordes, Tobias Eilert, Carel Fijen, C. Gebhardt, Markus Götz, Giorgos Gouridis, E. Gratton, T. Ha, Pengyu Hao, Christian A. Hanke, Andreas Hartmann, Jelle Hendrix, Lasse L. Hildebrandt, Verena Hirschfeld, J. Hohlbein, Boyang Hua, C. Hübner, E. Kallis, A. Kapanidis, Jae‐Yeol Kim, G. Krainer, D. Lamb, Nam Ki Lee, E. Lemke, Brié Levesque, M. Levitus, James J McCann, Nikolaus Naredi-Rainer, D. Nettels, Thuy T. M. Ngo, Ruoyi Qiu, Nicole C. Robb, C. Röcker, H. Sanabria, M. Schlierf, Tim Schröder, B. Schuler, H. Seidel, Lisa Streit, Johann Thurn, Philip Tinnefeld, S. Tyagi, Niels Vandenberk, A. M. Vera, K. Weninger, Bettina Wünsch, Inna S. Yanez-Orozco, Jens Michaelis, C. Seidel, Timothy D. Craggs, T. Hugel   +62 more
semanticscholar   +1 more source

Observations of intracellular tension dynamics of MC3T3-E1 cells during substrate adhesion using a FRET-based actinin tension sensor

Journal of Biomechanical Science and Engineering, 2016
Tension in actin filaments plays crucial roles in multiple cellular functions, although little is known about tension dynamics of cells during adhesion to substrates.
Junfeng WANG, Masahiro ITO, Wenhao ZHONG, Shukei SUGITA, Tatsuo MICHIUE, Takashi TSUBOI, Tetsuya KITAGUCHI, Takeo MATSUMOTO   +7 more
doaj   +1 more source

Nanoscale control of single molecule Förster resonance energy transfer by a scanning photonic nanoantenna

Nanophotonics, 2020
Förster Resonance Energy Transfer (FRET) is a widely applied technique in biology to accurately measure intra- and inter-molecular interactions at the nanometre scale.
Sanz-Paz Maria, Wenger Jerome, van Hulst Niek F., Mivelle Mathieu, Garcia-Parajo Maria F.   +4 more
doaj   +1 more source