Comparing the performance of mScarlet-I, mRuby3, and mCherry as FRET acceptors for mNeonGreen. [PDF]
PLoS ONE, 2020 Förster Resonance Energy Transfer (FRET) has become an immensely powerful tool to profile intra- and inter-molecular interactions. Through fusion of genetically encoded fluorescent proteins (FPs) researchers have been able to detect protein ...
Tyler W McCullock +2 more
doaj +1 more source
Principal factors that determine the extension of detection range in molecular beacon aptamer/conjugated polyelectrolyte bioassays. [PDF]
, 2015 A strategy to extend the detection range of weakly-binding targets is reported that takes advantage of fluorescence resonance energy transfer (FRET)-based bioassays based on molecular beacon aptamers (MBAs) and cationic conjugated polyelectrolytes (CPEs).
Bazan, Guillermo C +5 more
core +1 more source
Theory of resonance energy transfer involving nanocrystals: the role of high multipoles
, 2007 A theory for the fluorescence resonance energy transfer (FRET) between a pair of semiconducting nanocrystal quantum dots is developed. Two types of donor-acceptor couplings for the FRET rate are described: dipole-dipole (d-d) and the dipole-quadrupole (d-
Abramowitz M. +4 more
core +1 more source
A sensitive ERK fluorescent probe reveals the significance of minimal EGF-induced transcription
Cell Structure and FunctionExtracellular signal-regulated kinase (ERK) regulates multiple cellular functions through distinct activation patterns. Genetically encoded fluorescent probes are instrumental in dissecting the ERK activity dynamics in living cells.
Zhang Weisheng +4 more
doaj +1 more source
Integrative Biology, 2009 Since the development of green fluorescent protein (GFP) and other fluorescent proteins (FPs) with distinct colors, genetically-encoded probes and biosensors have been widely applied to visualize the molecular localization and activities in live cells.
Yingxiao, Wang, Ning, Wang
openaire +2 more sources
Capturing CDKs in action: Live-cell biosensors pioneer the new frontiers in cell cycle research
Cell Structure and FunctionCyclin-dependent kinases (CDKs) orchestrate cell cycle progression through precise temporal control of substrate phosphorylation. While traditional biochemical approaches and phosphoproteomics have provided valuable insights into CDK-mediated regulation,
Sachiya Nakashima +4 more
doaj +1 more source
CFP and YFP photostabilities are differentially affected by common mounting fluids [PDF]
, 2010 The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g.
Johannes A. Schmid
core +1 more source
Fretting about FRET: Correlation between κ and R [PDF]
Biophysical Journal, 2007 Molecular dynamics simulations were used to examine the structural dynamics of two fluorescent probes attached to a typical protein, hen egg-white lysozyme (HEWL). The donor probe (D) was attached via a succinimide group, consistent with the commonly-used maleimide conjugation chemistry, and the acceptor probe (A) was bound into the protein as occurs ...
VanBeek, Darren B. +3 more
openaire +2 more sources
Interaction of an Antituberculosis Drug with a Nanoscopic Macromolecular Assembly: Temperature-Dependent Förster Resonance Energy Transfer Studies on Rifampicin in an Anionic Sodium Dodecyl Sulfate Micelle [PDF]
, 2011 In this contribution, we report studies on the nature of binding of a potent antituberculosis drug, Rifampicin (RF) with a model drug delivery system, sodium dodecyl sulfate (SDS) micelle.
Makhal, Abhinandan +3 more
core
, 2011 In this work we monitor the catalytic mechanism of P-glycoprotein (Pgp) using single-molecule fluorescence resonance energy transfer (FRET). Pgp, a member of the ATP binding cassette family of transport proteins, is found in the plasma membrane of animal
Boersch, Michael +4 more
core +1 more source