Results 101 to 110 of about 5,001 (143)

Factors affecting fructosyltransferases and fructan exohydrolase activities in Agave tequilana Weber var. azul

Journal of Plant Biochemistry and Biotechnology, 2015
There is great interest in the fructosyltransferases (FTFs) involved in fructan metabolism and agents affecting their activity. Agaves accumulate fructans, fructose polymers linked by glycosidic β(2–1) and β(2–6) bonds in linear or branched configurations. In plants, fructans provide protection under stress conditions.
María Concepción García-Pérez, Mercedes G. López   +1 more
semanticscholar   +3 more sources

Fructan exohydrolase activity in leaves of Lolium temulentum L.

New Phytologist, 1991
summaryCrude extracts from leaves of Lolium temulentum L. exhibited invertase activity and activity capable of releasing fructost from fructans (fructan exohydrolase; FEH), The optimum pH for FEH activity was between pH 4.8 and 5.5. When shoots of 10‐d‐old seedlings were induced to accumulate fructans by cooling their roots to 5 °C and exposing them to
R. J. SIMPSON, R. P. WALKER, C. J. POLLOCK   +2 more
openaire   +2 more sources

Graminan breakdown by fructan exohydrolase induced in winter wheat inoculated with snow mold

Journal of Plant Physiology, 2012
Fructan structures vary widely among plant species. Graminan-type fructans, extensions of sucrose through β-(2,6)-linked fructosyl units with branches of β-(2,1)-linked fructosyl units, accumulate in tissues of winter wheat (Triticum aestivum) during cold hardening and are metabolized under persistent snow cover.
Akira, Kawakami, Midori, Yoshida
openaire   +3 more sources

Purification and Properties of Barley Stem Fructan Exohydrolase

Journal of Plant Physiology, 1989
Summary Barley, Hordeum vulgare(L.) Morex, stem fructan exohydrolase (FEH) has been partially purified andcharacterized. The enzyme was purified 258-fold by salt precipitation, anion exchange chromatography, affinity chromatography, and size exclusion chromatography.
C. Henson
openaire   +2 more sources

Fructan Exohydrolase from Lolium Rigidum Gaud

1993
ABSTRACT Fructan exohydrolase (FEH) was extracted from mature leaves and stems of Lolium rigidum Gaud. and partially purified by salt precipitation, affinity chromatography and anion exchange chromatography. The partially purified FEH was incubated with a range of substrates and found to be most active against fructan extracted from L.
G.D. BONNETT, R.J. SIMPSON
openaire   +2 more sources

Purification and properties of a second fructan exohydrolase from the roots of Cichorium intybus

Physiologia Plantarum, 1999
A 1‐FEH II (1‐fructan exohydrolase, EC 3.2.1.80) was purified from forced chicory roots (Cichorium intybus L. var. foliosum cv. Flash) by a combination of ammonium sulfate precipitation, concanavalin A (Con A) affinity chromatography and anion and cation exchange chromatography.
de Roover, J., van Laere, A., de Winter, M., Timmermans, J.W., van den Ende, W.   +4 more
openaire   +3 more sources

ENYMOLOGIC CHARACTERIZATION OF GARLIC FRUCTAN EXOHYDROLASE

Journal of Food Biochemistry, 2011
Fructans represent over 75% of the dry mass of garlic (Allium sativum L.). Understanding the enzymologic characteristics of garlic fructan exohydrolase (FEH) can provide theoretical basis on garlic germination inhibition and mass production of high ...
KIT LEONG CHEONG, FENGCHAO YAN, XUESONG HUANG   +2 more
openaire   +2 more sources

Characterization of a fructan exohydrolase purified from barley stems that hydrolyzes multiple fructofuranosidic linkages

Plant Physiology and Biochemistry, 1998
Abstract Barley ( Hordeum vulgare cv Morex) fructan exohydrolase (EC 3.2.1.80) was purified by precipitation with ammonium sulfate and chromatography on anion exchange and lectin affinity columns. The final enzyme preparation was homogenous as determined by the presence of a single band on silver stained SDS-PAGE and IEF gels.
Cynthia A. Henson, David P. Livingston
openaire   +2 more sources

Effect of temperature on fructan exohydrolase activity in leaves of Lolium perenne (L.)

Food Hydrocolloids, 1991
Abstract The relationship between fructan metabolism and the fructan exohydrolase (FEH) activity of ryegrass was studied as a function of thermoperiod. Cold transfer from a 25/15°C day/night regime to 5°C induced the increase of leaf carbohydrate contents, whereas the FEH activity was quite stable.
J. Arbillot, B. Gonzalez, O. Fontaine, J. Boucaud   +3 more
openaire   +2 more sources

Hexokinase-dependent sugar signaling represses fructan exohydrolase activity in Lolium perenne

2010
Defoliation of perennial ryegrass (Lolium perenne L.) by grazing animals leads to fructan mobilisation via an increase of fructan exohydrolase (FEH) activity. To highlight the regulation of fructan metabolism in perennial ryegrass, the role of sugars as signalling molecules for regulation of FEH activity after defoliation was evaluated.
Lothier, Jérémy, Lasseur, Bertrand, Prud'Homme, Marie-Pascale, Morvan-Bertrand, Annette   +3 more
openaire   +4 more sources