Results 21 to 30 of about 5,001 (143)

Characterization of a Fructan Exohydrolase from the Gut Bacterium <i>Anaerostipes butyraticus</i>. [PDF]

J Appl Glycosci (1999)
Glycoside hydrolase family 32 (GH32) enzymes play key roles in fructooligosaccharide metabolism in gut bacteria. In this study, a GH32 enzyme (GenBank code, GFO85652) containing carbohydrate binding module 66 (CBM66) from the gut bacterium Anaerostipes ...
Baula MAB, Sekine M, Kozono T, Nishikawa A, Kitano Y, Endo A, Tonozuka T.   +6 more
europepmc   +2 more sources

Integrated Transcriptomics and Metabolomics Analysis of the Fructan Metabolism Response to Low-Temperature Stress in Garlic. [PDF]

Genes (Basel), 2023
As the main reserve carbohydrate in garlic, fructan contributes to garlic’s yield and quality formation. Numerous studies have shown that plant fructan metabolism induces a stress response to adverse environments.
Bian H, Zhou Q, Du Z, Zhang G, Han R, Chen L, Tian J, Li Y.   +7 more
europepmc   +2 more sources

Safety evaluation of the food enzyme fructan β-fructosidase from the genetically modified Trichoderma reesei strain AR-577. [PDF]

EFSA J
Abstract The food enzyme fructan β‐fructosidase (β‐d‐fructan fructohydrolase; EC 3.2.1.80) is produced with the genetically modified Trichoderma reesei strain AR‐577 by AB Enzymes GmbH. The genetic modifications do not give rise to safety concerns. The food enzyme is free from viable cells of the production organism and its DNA.
EFSA Panel on Food Enzymes (FEZ), Zorn H, Barat Baviera JM, Bolognesi C, Catania F, Gadermaier G, Greiner R, Mayo B, Mortensen A, Roos YH, Solano MLM, Sramkova M, Van Loveren H, Vernis L, Chesson A, Herman L, Kovalkovičová N, Aguilera J, Andryszkiewicz M, Cavanna D, Liu Y.   +20 more
europepmc   +2 more sources

Simultaneous Knockout of Tk1-SST and Tk1-FFT via CRISPR/Cas9 Enhances the Natural Rubber Accumulation in Taraxacum kok-saghyz. [PDF]

Plant Biotechnol J
ABSTRACT Taraxacum kok‐saghyz (TKS) synthesises natural rubber (NR) and inulin using sucrose as a carbon source. However, molecular mechanisms regulating inulin and NR accumulation remain largely unclear. Here, we report the generation of double‐gene homozygous mutants, 1‐sst1‐fft, by simultaneously knocking out two key genes responsible for inulin ...
Qin B, Hou X, Fan S, Li C, Wang X, Chen Q, Yang Y, Lin P, Lu LR, Fu C, Yang N, Gao Q, Zheng S, Liu S.   +13 more
europepmc   +2 more sources

Laminaria digitata Extract Improved Leaf Meristem Protection Under Drought and Nitrogen Uptake After Rehydration Through Hormesis-Based Chemical Priming in Lolium perenne. [PDF]

Plant Cell Environ
ABSTRACT Drought is among the most damaging stress for plants, impacting crop yield and grassland sustainability. This study aimed to evaluate the biostimulant effect of an algal extract from Laminaria digitata on Lolium perenne cultivated in a growth chamber. Leaves were sprayed at different concentrations 7 days before stopping irrigation.
Grandin-Courbet A, Morvan-Bertrand A, Dehail M, Hennequart F, Prud'homme MP.   +4 more
europepmc   +2 more sources

Viral-Based Gene Editing System for Nutritional Improvement of Fructan Content in Lettuce. [PDF]

Int J Mol Sci
Lettuce is a globally cultivated and consumed leafy crop. Here we developed an efficient tobacco rattle virus (TRV)-based guide RNA (gRNA) delivery system for CRISPR/Cas editing in the commercial lettuce cultivar ‘Noga’.
Livneh Y, Agmon D, Leor-Librach E, Vainstein A.   +3 more
europepmc   +2 more sources

Fructan exohydrolase in non fructan plants [PDF]

Plant, Cell & Environment, 2007
ABSTRACTThe genome of Arabidopsis thaliana contains six putative cell‐wall type invertase genes (AtcwINV1‐6). Heterologous expression of AtcwINV1, 3 and 6 cDNAs in Pichia pastoris revealed that the enzymes encoded by AtcwINV3 and 6 did not show invertase activity.
BARBARA DE CONINCK, KATRIEN LE ROY, ISOLDE FRANCIS, STEFAN CLERENS, RUDY VERGAUWEN, AILEEN M. HALLIDAY, STEVEN M. SMITH, ANDRÉ VAN LAERE, WIM VAN DEN ENDE   +8 more
openaire   +2 more sources

Defoliation Induces Fructan 1-Exohydrolase II in Witloof Chicory Roots. Cloning and Purification of Two Isoforms, Fructan 1-Exohydrolase IIa and Fructan 1-Exohydrolase IIb. Mass Fingerprint of the Fructan 1-Exohydrolase II Enzymes [PDF]

Plant Physiology, 2001
Abstract The cloning of two highly homologous chicory (Cichorium intybus var. foliosum cv Flash) fructan 1-exohydrolase cDNAs (1-FEH IIa and 1-FEH IIb) is described. Both isoenzymes could be purified from forced chicory roots as well as from the etiolated “Belgian endive” leaves where the 1-FEH IIa isoform is present in higher ...
W, Van den Ende, A, Michiels, D, Van Wonterghem, S P, Clerens, J, De Roover, A J, Van Laere   +5 more
openaire   +2 more sources

Identification and characterization of inulinases by bioinformatics analysis of bacterial glycoside hydrolases family 32 (GH32)

Engineering in Life Sciences, Volume 23, Issue 8, August 2023., 2023
Abstract The glycoside hydrolase family contains enzymes that break the glycosidic bonds of carbohydrates by hydrolysis. Inulinase is one of the most important industrial enzymes in the family of Glycoside Hydrolases 32 (GH32). In this study, to identify and classify bacterial inulinases initially, 16,002 protein sequences belonging to the GH32 family ...
Fatemeh Khosravi, Ehsan Mohseni Fard, Marzieh Hosseininezhad, Hadi Shoorideh   +3 more
wiley   +1 more source

Improving crop yield potential: Underlying biological processes and future prospects

Food and Energy Security, Volume 12, Issue 1, January 2023., 2023
The manuscript is part of a review collection from the CropBooster‐P project (https://www.cropbooster‐p.eu/). In this review, we present an overview of some of the distinct biological processes considered to be crucial for yield determination and recent updates in the respective fields.
Alexandra J. Burgess, Céline Masclaux‐Daubresse, Günter Strittmatter, Andreas P. M. Weber, Samuel Harry Taylor, Jeremy Harbinson, Xinyou Yin, Stephen Long, Matthew J. Paul, Peter Westhoff, Francesco Loreto, Aldo Ceriotti, Vandasue L. R. Saltenis, Mathias Pribil, Philippe Nacry, Lars B. Scharff, Poul Erik Jensen, Bertrand Muller, Jean‐Pierre Cohan, John Foulkes, Peter Rogowsky, Philippe Debaeke, Christian Meyer, Hilde Nelissen, Dirk Inzé, René Klein Lankhorst, Martin A. J. Parry, Erik H. Murchie, Alexandra Baekelandt   +28 more
wiley   +1 more source