Results 181 to 190 of about 2,602 (201)

Fructosyltransferase Enzymes for Microbial Fructan Production

2020
Fructans are fructose-based oligo- and polysaccharides synthesized using sucrose as substrate. Depending on the glycosidic bonds in their structure, they are classified as inulin and levan types or a mixture of these, namely graminans and agavins.
Nazlıcan Tezgel, Onur Kırtel, Wim Van den Ende, Ebru Toksoy Oner   +3 more
openaire   +1 more source

Microbial production of fructosyltransferases for synthesis of pre-biotics

Biotechnology Letters, 2008
Fructooligosaccharides (FOS) are prebiotic substances found in several vegetable or natural foods. The main commercial production of FOS comes from enzymatic transformation of sucrose by the microbial enzyme fructosyltransferase. The development of more efficient enzymes, with high activity and stability, is required and this has attracted the interest
Alfredo Eduardo, Maiorano, Rosane Moniz, Piccoli, Elda Sabino, da Silva, Maria Filomena, de Andrade Rodrigues   +3 more
openaire   +2 more sources

Expression and Activity Analysis of Fructosyltransferase from Aspergillus oryzae

The Protein Journal, 2017
The fructosyltransferase gene was isolated and cloned from Aspergillus oryzae. The gene was 1368 bp, which encoded a protein of 455 amino acids. To analyze the activity of the expressed fructosyltransferase, the pET32a-fructosyltransferase recombined plasmid was transformed into Escherichia coli BL21.
Lihong, Guan, Liping, Chen, Yongsen, Chen, Nu, Zhang, Yawei, Han   +4 more
openaire   +2 more sources

Isolation and characterization of fructosyltransferase from chicory roots

Phytochemistry, 1971
Abstract The occurrence of fructosyltransferase was demonstrated in the roots of chicory. The enzyme was purified 14-fold by ammonium sulphate fractionation and sephadex column chromatography. The optimal conditions for activity were pH 5·6, 37° and substrate (sucrose) concentration of 5·84 × 10 −1 M.
Randhir Singh, I.S. Bhatia
openaire   +1 more source

Novel Fructosyltransferases

2001
The present invention describes two novel proteins having fructosyltransferase activity. Both enzymes are derived from lactobacilli, which are food-grade microorganisms with the Generally Recognized As Safe (GRAS) status. One of these proteins produces an inulin and fructo-oligosaccharides, while the other produces a levan.
Geel-Schutten, G.H. van, Rahaoui, H., Dijkhuizen, L., Hijum, S.A.F.T. van   +3 more
openaire   +1 more source

Purification and kinetic characterization of a fructosyltransferase from Aspergillus aculeatus

Journal of Biotechnology, 2007
A fructosyltransferase present in Pectinex Ultra SP-L, a commercial enzyme preparation from Aspergillus aculeatus, was purified to 107-fold and further characterised. The enzyme was a dimeric glycoprotein (20% (w/w) carbohydrate content) with a molecular mass of around 135 kDa for the dimer.
Iraj, Ghazi, Lucia, Fernandez-Arrojo, Humberto, Garcia-Arellano, Manuel, Ferrer, Antonio, Ballesteros, Francisco J, Plou   +5 more
openaire   +2 more sources

Expression of fructosyltransferase genes in transgenic plants

1999
Fructans serve as a carbohydrate reserve in many plant species and are also synthesised by several microorganisms. Over the past decade interest in the use of fructans for food and non-food applications has increased exponentially. Our interest is to modify crops for the production of tailor-made fructans.
Vijn, I., van Dijken, A., Turk, S., Ebskamp, M., van Dun, K., Weisbeek, P., Smeekens, S.   +6 more
openaire   +3 more sources

Cloning of 1 Fructan: Fructan Fructosyltransferase Gene and Expression of Recombinant 1 Fructan: Fructan Fructosyltransferase in Yeast

2016
38 (Supplement), 21, KKU Research ...
Budsaraporn Ngampanya, Kriengsak Boonchoo   +1 more
openaire   +1 more source

Adsorption equilibrium of fructosyltransferase on a weak anion-exchange resin

Journal of Chromatography A, 2007
The adsorption equilibrium of a glycoprotein, fructosyltransferase from Aureobasidium pullulans, on an anion-exchange resin, Sepabeads FP-DA activated with 0.1M NaOH, was investigated. The adsorption isotherms were determined at 20 degrees C in a phosphate-citrate buffer with pH 6.0 using the static method.
Katarína, Vanková, Monika, Antosová, Milan, Polakovic   +2 more
openaire   +2 more sources

Cranberry constituents affect fructosyltransferase expression in Streptococcus mutans.

Alternative therapies in health and medicine, 2009
Cranberry juice has long been recognized in folk medicine as a therapeutic agent, mainly in urinary tract infections. Its proposed mechanism of action is antiadhesion of bacteria.Investigation of the potential antiadhesion effect of nondialyzed material of cranberry (NDM) via its influence on secretion, gene expression, and promoter activity of the ...
Mark, Feldman, Ervin, Weiss, Moshe, Shemesh, Itzhak, Ofek, Gilad, Bachrach, Ramona, Rozen, Doron, Steinberg   +6 more
openaire   +1 more source