Results 161 to 170 of about 2,039 (203)
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Immobilization of a commercial Aspergillus aculeatus enzyme preparation with fructosyltransferase activity in chitosan beads: A kinetic/thermodynamic study and fructo-oligosaccharides continuous production in enzymatic reactor

, 2020
Pectinex Ultra SP-L, a food-grade enzymatic preparation with high transfructosylating activity, was covalently immobilized on chitosan beads. The highest immobilization yield (95.9%) was obtained using 4.0% glutaraldehyde for 60 min and 80 rpm at 25 ± 1 °
R. L. C. Oliveira   +5 more
semanticscholar   +1 more source

A Comparative Study on Immobilization of Fructosyltransferase in Biodegradable Polymers by Electrospinning

Applied Biochemistry and Biotechnology, 2018
Commercial application of biocatalysts depends on the efficiency of the immobilization method and residual enzyme activity. Electrospinning offers a simple and versatile route to immobilize enzymes in submicron-sized fibers and thus improved mass transfer characteristics.
Jakub Gabrielczyk   +4 more
openaire   +2 more sources

Adsorption equilibrium of fructosyltransferase on a weak anion-exchange resin

Journal of Chromatography A, 2007
The adsorption equilibrium of a glycoprotein, fructosyltransferase from Aureobasidium pullulans, on an anion-exchange resin, Sepabeads FP-DA activated with 0.1M NaOH, was investigated. The adsorption isotherms were determined at 20 degrees C in a phosphate-citrate buffer with pH 6.0 using the static method.
Katarína, Vanková   +2 more
openaire   +2 more sources

Purification and biochemical characteristics of a novel fructosyltransferase with a high FOS transfructosylation activity from Aspergillus oryzae S719.

Protein Expression and Purification, 2019
Fructooligosaccharides (FOS) have widely used for the manufacture of low-calorie and functional foods, because they can inhibit intestinal pathogenic microorganism growth and increase the absorption of Ca2+ and Mg2+.
Susu Han   +6 more
semanticscholar   +1 more source

Enhanced production of fructosyltransferase in Aspergillus oryzae by genome shuffling

Biotechnology Letters, 2016
To breed Aspergillus oryzae strains with high fructosyltransferase (FTase) activity using intraspecific protoplast fusion via genome-shuffling.A candidate library was developed using UV/LiCl of the conidia of A. oryzae SBB201. By screening for enzyme activity and cell biomass, two mutants (UV-11 and UV-76) were chosen for protoplast fusion and ...
Shenghai, Wang   +5 more
openaire   +2 more sources

Computational analysis of the fructosyltransferase enzymes in plants, fungi and bacteria

Gene, 2011
Fructosyltransferases (FTases) are enzymes produced by plants, fungi, and bacteria, which are responsible for the synthesis of fructooligosaccharides. In this study, we conducted a computational analysis of reported sequences for FTase from a diverse source of organisms, such as plants, fungi, and bacteria.
Carlos J, Alméciga-Díaz   +5 more
openaire   +2 more sources

Isolation and characterization of fructosyltransferase from chicory roots

Phytochemistry, 1971
Abstract The occurrence of fructosyltransferase was demonstrated in the roots of chicory. The enzyme was purified 14-fold by ammonium sulphate fractionation and sephadex column chromatography. The optimal conditions for activity were pH 5·6, 37° and substrate (sucrose) concentration of 5·84 × 10 −1 M.
Randhir Singh, I.S. Bhatia
openaire   +1 more source

Rational design and immobilization of a recombinant sucrose:sucrose 1-fructosyltransferase on Sepabeads® and ReliZymeTM supports for short-chain fructooligosaccharides production

Process Biochemistry, 2023
D. Martínez   +6 more
semanticscholar   +1 more source

Fructosyltransferase production by Aspergillus oryzae BM-DIA using solid-state fermentation and the properties of its nucleotide and protein sequences

Folia Microbiologica (Prague), 2021
Mariela R. Michel   +6 more
semanticscholar   +1 more source

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