Results 41 to 50 of about 194,325 (281)

The Effectiveness of PCR in Diagnosis of Fungal Keratitis

Oftalʹmologiâ, 2020
Fungi implicated in mycotic keratitis include different species. Conventional methods for the diagnosis of fungal keratitis include staining of corneal scarpings, culture medium (Sabouraud agar) for isolating fungi.Purpose.
G. I. Krichevskaya, L. A. Kovaleva, I. D. Zyurnyayeva, P. V. Makarov, A. E. Andryushin   +4 more
doaj   +1 more source

Evaluating the Impact of DNA Extraction Method on the Representation of Human Oral Bacterial and Fungal Communities. [PDF]

PLoS ONE, 2017
The application of high-throughput, next-generation sequencing technologies has greatly improved our understanding of the human oral microbiome. While deciphering this diverse microbial community using such approaches is more accurate than traditional ...
Anna Vesty, Kristi Biswas, Michael W Taylor, Kim Gear, Richard G Douglas   +4 more
doaj   +1 more source

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source

SWI/SNF complex-mediated chromatin remodeling in Candida glabrata promotes immune evasion

iScience
Summary: Immune evasion is critical for fungal virulence. However, how the human opportunistic pathogen Candida glabrata (Cg) accomplishes this is unknown. Here, we present the first genome-wide nucleosome map of the macrophage-internalized Cg consisting
Kundan Kumar, Aditi Pareek, Rupinder Kaur   +2 more
doaj   +1 more source

DNA- and RNA- Derived Fungal Communities in Subsurface Aquifers Only Partly Overlap but React Similarly to Environmental Factors

Microorganisms, 2019
Recent advances in high-throughput sequencing (HTS) technologies have revolutionized our understanding of microbial diversity and composition in relation to their environment.
Ali Nawaz, Witoon Purahong, Martina Herrmann, Kirsten Küsel, François Buscot, Tesfaye Wubet   +5 more
doaj   +1 more source

Time after time – circadian clocks through the lens of oscillator theory

FEBS Letters, EarlyView.
Oscillator theory bridges physics and circadian biology. Damped oscillators require external drivers, while limit cycles emerge from delayed feedback and nonlinearities. Coupling enables tissue‐level coherence, and entrainment aligns internal clocks with environmental cues.
Marta del Olmo, Carolin Ector, Hanspeter Herzel   +2 more
wiley   +1 more source

Protocol for determination of phosphatidylinositol 3-phosphate levels and localization in Candida glabrata by confocal microscopy

STAR Protocols
Summary: Phosphatidylinositol 3-phosphate (PI3P) levels govern membrane trafficking in Candida glabrata. Here, we present a confocal imaging-based protocol for PI3P localization analysis using the GFP-FYVE (found in Fab1, YOTB, Vac1, and EEA1) fusion ...
Fizza Askari, Rupinder Kaur
doaj   +1 more source

Internal Transcribed Spacer (ITS) as Dna Barcoding to Identify Fungal Species: a Review

Squalen, 2016
Despite the fact that fungi are important sources of both bioactive compounds and mycotoxins, and that they are very ubiquitous in our environment, their species identification is hampered by incomplete and often unclear literature.
Nurrahmi Dewi Fajarningsih
doaj   +1 more source

Potential therapeutic targeting of BKCa channels in glioblastoma treatment

Molecular Oncology, EarlyView.
This review summarizes current insights into the role of BKCa and mitoBKCa channels in glioblastoma biology, their potential classification as oncochannels, and the emerging pharmacological strategies targeting these channels, emphasizing the translational challenges in developing BKCa‐directed therapies for glioblastoma treatment.
Kamila Maliszewska‐Olejniczak, Karolina Pytlak, Sandra Jaworowska, Bogusz Kulawiak, Piotr Bednarczyk   +4 more
wiley   +1 more source

Aspartyl proteases target host actin nucleator complex protein to limit epithelial innate immunity

EMBO Reports
Epithelial-immune cell communication is pivotal to control microbial infections. We show that glycosylphosphatidylinositol-linked aspartyl proteases (Yapsins) of the human opportunistic pathogenic yeast Candida glabrata (Cg) thwart epithelial cell (EC ...
Sandip Patra, Rupinder Kaur
doaj   +1 more source