Results 61 to 70 of about 15,824,629 (262)

Fusion protein strategies for cryo-EM study of G protein-coupled receptors

Nature Communications, 2022
Here, Zhang et al. explore fusion protein strategies to facilitate cryo-EM structural studies of GPCRs alone- without signal transducers- in ligand bound or unliganded form.
Kaihua Zhang, Hao Wu, Nicholas Hoppe, Aashish Manglik, Yifan Cheng   +4 more
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

GPCRTree: online hierarchical classification of GPCR function [PDF]

, 2008
Background: G protein-coupled receptors (GPCRs) play important physiological roles transducing extracellular signals into intracellular responses. Approximately 50% of all marketed drugs target a GPCR.
Davies Matthew N, Clark, Edward, Halling-Brown Mark, Flower Darren R, Moss, David S., Alex A Freitas, Halling-Brown, Mark D., Clark, E., Timmis Jon, Flower, Darren R, Andrew Secker, Freitas, Alex A., Timmis, J., Davies, M.N., David S Moss, Secker, Andrew D., Secker, A., Halling-Brown, Mark, Moss David S, Timmis, Jon, Freitas, A.A., Mark Halling-Brown, Clark Edward, Secker Andrew, Secker, Andrew, Flower, Darren R., Jon Timmis, Edward Clark, Matthew N Davies, Freitas Alex A, Davies, Matthew N., Darren R Flower, Flower, D.R.   +32 more
core   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Phylogenomic analysis reveals extensive phylogenetic mosaicism in the Human GPCR Superfamily [PDF]

, 2007
A novel high throughput phylogenomic analysis (HTP) was applied to the rhodopsin G-protein coupled receptor (GPCR) family. Instances of phylogenetic mosaicism between receptors were found to be frequent, often as instances of correlated mosaicism and ...
Allaby, Robin G., Woodwark, Mathew, Robin G. Allaby, Mathew Woodwark   +3 more
core   +1 more source

Stabilization of G protein-coupled receptors by point mutations

Frontiers in Pharmacology, 2015
G protein-coupled receptors (GPCRs) are flexible integral membrane proteins involved in transmembrane signaling. Their involvement in many physiological processes makes them interesting targets for drug development.
Franziska eHeydenreich, Franziska eHeydenreich, Ziva eVuckovic, Ziva eVuckovic, Milos eMatkovic, Milos eMatkovic, Dmitry eVeprintsev, Dmitry eVeprintsev   +7 more
doaj   +1 more source

Agonist and antagonist TRUPATH assays for G protein-coupled receptors

STAR Protocols, 2022
Summary: TRUPATH is a bioluminescence resonance energy transfer-based platform for quantifying G protein-coupled receptor activity via dissociation of heterotrimeric G protein biosensors.
Jeffrey F. DiBerto, Katie Smart, Reid H.J. Olsen, Bryan L. Roth   +3 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

G protein-coupled receptor kinase 2-mediated phosphorylation of ezrin is required for G protein-coupled receptor-dependent reorganization of the actin cytoskeleton [PDF]

, 2005
G protein-coupled receptor kinase 2 (GRK2) phosphorylates and desensitizes activated G protein-coupled receptors (GPCRs). Here, we identify ezrin as a novel non-GPCR substrate of GRK2. GRK2 phosphorylates glutathione S-transferase (GST)-ezrin, but not an
Pitcher, JA, Cant, SH
core  

G-protein coupled receptor structure

Biochimica et Biophysica Acta (BBA) - Biomembranes, 2007
Because of their central role in regulation of cellular function, structure/function relationships for G-protein coupled receptors (GPCR) are of vital importance, yet only recently have sufficient data been obtained to begin mapping those relationships. GPCRs regulate a wide range of cellular processes, including the senses of taste, smell, and vision,
Yeagle, Philip L., Albert, Arlene D.
openaire   +2 more sources