Results 91 to 100 of about 1,006,409 (430)
Hermansky‐Pudlak syndrome type 1 (HPS‐1) is a rare, autosomal recessive disorder with poorly understood renal involvement. Urinary extracellular vesicle (uEV) proteomics and a novel Hps1 mouse model reveal mitochondrial abnormalities and lipid accumulation in HPS‐1 kidney proximal tubule cells. Serum ApoA1 correlates with kidney function in our patient
Dawn M. Maynard+7 more
wiley +1 more source
G-Protein–Coupled Receptors in Heart Disease
GPCRs (G-protein [guanine nucleotide-binding protein]–coupled receptors) play a central physiological role in the regulation of cardiac function in both health and disease and thus represent one of the largest class of surface receptors targeted by drugs.
Jialu Wang, C. Gareri, H. Rockman
semanticscholar +1 more source
We describe a novel set of Epac‐based FRET‐FLIM biosensors with improved fully cytosolic distribution, achieved without compromising the state‐of‐the‐art performance of our original designs, for detecting cAMP dynamics in real time in live cells with high precision and reliability.
Giulia Zanetti+2 more
wiley +1 more source
Quantifying selection in immune receptor repertoires [PDF]
The efficient recognition of pathogens by the adaptive immune system relies on the diversity of receptors displayed at the surface of immune cells. T-cell receptor diversity results from an initial random DNA editing process, called VDJ recombination, followed by functional selection of cells according to the interaction of their surface receptors with
arxiv +1 more source
The mitochondrial outer membrane iron–sulphur ([Fe‐S]) protein mitoNEET is a target of the type‐2 diabetes drug pioglitazone. Its unknown molecular function is linked to respiratory complex activity and mitochondrial function. We discovered that O2 protects the mitoNEET [2Fe‐2S] cluster against NO oxidation and desensitization towards reduction by H2S.
Thao Nghi Hoang+9 more
wiley +1 more source
Rules and mechanisms governing G protein coupling selectivity of GPCRs
Summary: G protein-coupled receptors (GPCRs) convert extracellular stimuli into intracellular signaling by coupling to heterotrimeric G proteins of four classes: Gi/o, Gq, Gs, and G12/13.
Ikuo Masuho+8 more
doaj
Fusion protein strategies for cryo-EM study of G protein-coupled receptors
Here, Zhang et al. explore fusion protein strategies to facilitate cryo-EM structural studies of GPCRs alone- without signal transducers- in ligand bound or unliganded form.
Kaihua Zhang+4 more
doaj +1 more source
Local Clustering and Global Spreading of Receptors for Optimal Spatial Gradient Sensing [PDF]
Spatial information from cell-surface receptors is crucial for processes that require signal processing and sensing of the environment. Here, we investigate the optimal placement of such receptors through a theoretical model that minimizes uncertainty in gradient estimation.
arxiv +1 more source
An allosteric role for receptor activity-modifying proteins in defining GPCR pharmacology [PDF]
G protein-coupled receptors are allosteric proteins that control transmission of external signals to regulate cellular response. Although agonist binding promotes canonical G protein signalling transmitted through conformational changes, G protein ...
A Christopoulos+47 more
core +1 more source
We explore 5HT2A receptor activation, a key target for psychedelic drugs, using molecular dynamics simulations. Without Gqα, the active receptor collapses to an inactive conformation, underscoring the G‐protein's role. Discovery of an intermediate “partially‐open” state and the higher affinity of ligands to the orthosteric binding site provides ...
Niklas Viohl+2 more
wiley +1 more source